A second device includes a first surface, a second surface in contact with a first device, and a first hole extending through and between the first surface and the second surface and being continuous with a groove on the first device. A third device includes a third surface in contact with the first surface, a second hole open in the third surface and continuous with the first hole, and a flow path continuous with the second hole and open in the third surface. As viewed in a first direction from the first surface to the second surface, the first hole includes at least one vertex surrounded by the second hole, and a pair of sides joined to the at least one vertex and widening toward the flow path to define a minor angle.

A system, mixing-enhanced microfluidic container, and methods for small volume sample collection and/or analysis is disclosed. Namely, the invention is directed to a small volume sample collection system that includes a mixing-enhanced microfluidic container and a durable reusable actuation chuck. The mixing-enhanced microfluidic container is used to collect small volumes of sample fluid and includes a means for mixing the sample fluid with reagents disposed within the microfluidic container. The mixing means utilize an array of surface-attached structures (e.g., a micropost array). The application of an “actuation force,” such as a magnetic or electric field, actuates the surface-attached structures into movement, wherein the actuation chuck in close proximity to the mixing-enhanced microfluidic container provides the “actuation force.”

Provided are a method and a device for encapsulating a cell in droplet for single-cell analysis, or a method and a device for forming droplet for single-cell analysis. According to the method and the device of one aspect, by using the effects of inertial ordering, not only a ratio at which one cell is encapsulated in one droplet is increased, but also a yield of generating droplet is improved.

In an electrochemical lateral flow immunological test method, flow of a sample solution is controlled. As a result, the reaction time is short and quantitative measurements and electrical measurements can be performed with excellent sensitivity and high accuracy, and the invention provides a sensor employed in the method. Electrode portions, electrically conductive portions for transferring electric current from the electrode portions, and connecting portions connected to an electrical measuring instrument for measuring the electric current values are arranged on a supporting body including a resin sheet, pads and the like disposed by partial lamination on the supporting body. A sample solution flows over the plurality of pads, and electrochemical detection is performed by controlling the flow at the position of the electrode portions. Furthermore, the flow is controlled by a flow rate control pad, a flow passage portion fiber pad, and flow rate control protruding portions.

A liquid handling device includes: a cartridge including a first reservoir part in which a first reagent that is preservable in a non-frozen state is stored; and a channel chip including a second reservoir part in which a second reagent that should be preserved in a frozen state is stored, and a channel connected to the second reservoir part. The cartridge is attachable and detachable to and from the channel chip. The first reservoir part is connected to the channel when the cartridge is mounted in the channel chip.

Systems, methods, and techniques are disclosed herein for isolating rare cells and clusters of cells, such as CTCs, from large volumes of sample fluids, such as whole blood, diluted blood, e g, minimally diluted blood, and other samples such as leukapheresis and aphaeresis samples. In some implementations, a microfluidic device includes a particle enrichment module and a particle separation module for iterative multistage sorting. Each module can have an array of islands in a microfluidic channel having a sample inlet at a first end of the first microfluidic channel. The array of islands is arranged in one or more rows that extend along a longitudinal direction in the microfluidic channel. Each island in a row is spaced apart from an adjacent island in the row to form a siphoning channel. The array of islands is configured and arranged to shift portions of fluid through the siphoning channel between adjacent islands.

Devices, kits, and their methods of use for lysing and/or purifying biological particles, e.g., nuclei are provided. One or more thixotropic layers can be employed in a vessel to purify biological particles. A device with sharp features may be employed to lyse biological particles or the contents thereof.

Described herein are devices and methods for high throughput purification of particles. In some cases, methods and devices described herein can be used to remove erythrocytes and purify leukocytes and raise the quality of umbilical cord blood and other transplant grafts, thereby significantly improving patient outcomes.

A catcher, a capture device, and a method for capturing at least one target biological particle are provided. The catcher includes a base and a plurality of capture arms extending from the base and spaced apart from each other. Each of the capture arms has a free end portion configured to capture a target biological particle and a supporting segment connected between the free end portion and the base. The supporting segment of each of the capture arms is arranged in a projection space defined by orthogonally projecting the free end portion along a height direction onto the base. When the target biological particle is captured by two of the capture arms that are bent and arranged adjacent to each other, a part of the target biological particle is trapped by the supporting segments of the two of the capture arms and is held.

A system, configured to facilitate processing and detection of nucleic acids, the system comprising a process fluid container and a cartridge comprising: a top layer, a set of sample port-reagent port pairs, a shared fluid port, a vent region, a heating region, and a set of detection chambers; an intermediate substrate, coupled to the top layer comprising a waste chamber; an elastomeric layer, partially situated on the intermediate substrate; and a set of fluidic pathways, each formed by at least a portion of the top layer and a portion of the elastomeric layer, wherein each fluidic pathway is fluidically coupled to a sample port-reagent port pair, the shared fluid port, and a detection chamber, comprises a portion passing through the heating region, and is configured to be occluded upon deformation of the elastomeric layer, to transfer a waste fluid to the waste chamber, and to pass through the vent region.