Disclosed herein is a device for reducing the volume of an aquatic sample, comprising, a substrate; a metal layer disposed above the substrate; a hydrophobic layer disposed above the metal layer having a plurality of assay wells formed therein; and a hydrophilic layer coated on each of the plurality of assay wells. Also encompassed in the present disclosure are a kit comprising the device and a lipoplex containing a liposome and a fluorescence-labeled molecular beacon inside the liposome, and use of the kit in detecting a target nucleic acid in a biological sample.

A method includes 3d printing a polymer substrate having microfluidic channels and depositing calcite onto the polymer substrate then using atomic layer deposition to form a calcite microfluidic device. A device made from the method includes a 3d printed polymer substrate having microfluidic channels. The 3d printed polymer substrate has a calcite coating.

A device for manipulating a droplet comprising water is provided, the device including: (i) a surface configured to support the droplet, the surface including a hydrophobic region; and (ii) an ultrasound transducer array, the ultrasound transducer array being arranged above the surface and separated from the surface; wherein the ultrasound transducer array is configured to emit ultrasound for actuating a motion of the droplet along the surface by subjecting the droplet to an acoustic radiation force by the emitted ultrasound.

This disclosure describes a reaction vessel assembly that includes the following: a reaction vessel including a housing component; a reaction chamber defined by the housing component; and a light absorbing layer conforming to a portion of an interior-facing surface of the housing component that defines the reaction chamber, the light absorbing layer comprising a multiple discrete regions; and an energy source configured to direct light through at least a portion of the housing component at one or more of the discrete regions of the light absorbing layer.

The detection chip includes a first substrate, a micro-cavity defining layer, a hydrophilic layer, and a hydrophobic layer. The micro-cavity defining layer is on the first substrate and defines a plurality of micro-reaction chambers. Each of the plurality of micro-reaction chambers includes a reaction trap, and the reaction trap includes a sidewall and a bottom. The micro-cavity defining layer includes a spacing region between the plurality of micro-reaction chambers, and the spacing region includes a first region adjacent to the sidewall, and a second region non-adjacent to the sidewall. The hydrophilic layer covers the sidewall and the bottom of each of the plurality of micro-reaction chambers, and the hydrophobic layer covers the second region.

Methods and devices for evaluating coagulation are described, including methods and devices for detecting an anticoagulant agent or a coagulation abnormality. In various embodiments, the methods and devices of the invention measure coagulation of a sample in response to a gradient of one or more coagulation factors. These responses can be evaluated to accurately profile coagulation impairments of the sample, including the presence of anticoagulant medication. In various embodiments, the invention provides point-of-care or bedside testing with a convenient, microfluidic device that can be used by minimally trained personnel.

The present disclosure provides a cell harvesting method and device for the efficient sedimentation and retention of cells from liquid samples onto a solid support with low cell losses and low impact on cell morphology. The device has two configurations, one being for use in a centrifuge to centrifuge the sample, a second for controlled release of the liquid post centrifugation. The device includes a base to hold a solid support that receives cells on a top surface thereof that releasably holds a fluid chamber which has a first opening with a gasket surrounding the opening with that bears against a top surface of the support. An opening receives the liquid sample and the gasket defines an area into which the targeted cells deposit. A first cap closes the second opening during centrifugation. Post centrifugation the first cap is removed and a fluid absorbing element and cap, designed to provide controlled removal of the liquid and to prevent positioning of a tip of the absorbing element to a distance from the solid support shorter than a predefined distance is inserted into the fluid chamber.

A microfluidic device for detection of an analyte in a fluid is described. The microfluidic device comprises a substrate having a first surface defining entrances to one or more chambers defined in the substrate, surfaces of the chambers defining a second surface of the substrate, the first surface being modified for selective targeting and capture of at least one analyte to operably effect a blocking of the entrance to at least one of the chambers, and wherein a response characteristic of the microfluidic device is operably varied by the blocking of the entrance to the at least one of the chambers, thereby providing an indication of the presence of the analyte within the fluid.

Provided are valveless microfluidic flowchips comprising fluid flow barrier structures or configurations. Further provided are systems and methods having increased fluid transfer control in a valveless microfluidic flowchip. The systems and methods can be used in the present valveless microfluidic flowchips as well as in currently available valveless microfluidic flowchips.

The present disclosure provides a cell harvesting method and device for the efficient sedimentation and retention of cells from liquid samples onto a solid support with low cell losses and low impact on cell morphology. The device has two configurations, one being for use in a centrifuge to centrifuge the sample, a second for controlled release of the liquid post centrifugation. The device includes a base to hold a solid support that receives cells on a top surface thereof that releasably holds a fluid chamber which has a first opening with a gasket surrounding the opening with that bears against a top surface of the support. An opening receives the liquid sample and the gasket defines an area into which the targeted cells deposit. A first cap closes the second opening during centrifugation. Post centrifugation the first cap is removed and a fluid absorbing element and cap, designed to provide controlled removal of the liquid and to prevent positioning of a tip of the absorbing element to a distance from the solid support shorter than a predefined distance is inserted into the fluid chamber.