A magnetic-field generator (10) is provided to be used in construction of a cell sheet (12), and include s a magnetic circuit assembly (24) configured to generate a magnetic field for the construction of the cell sheet (12) using magnetic force caused by the magnetic field wherein the magnetic force is substantially transverse to a plane defined by the cell sheet (12), and a power control system (44) configured to generate and control electric power to magnetically charge the magnetic circuit assembly (24) using the electric power.

An engine-driven cryogenic cooling system for an aircraft includes a first air cycle machine, a second air cycle machine, and a means for condensing a chilled air stream into liquid air for an aircraft use. The first air cycle machine includes a plurality of components operably coupled to a gearbox of a gas turbine engine and configured to produce a cooling air stream based on a first engine bleed source of the gas turbine engine. The second air cycle machine is operable to output the chilled air stream at a cryogenic temperature based on a second engine bleed source cooled by the cooling air stream of the first air cycle machine.

A separating apparatus of biosubstance and a separating method of the same are provided. The separating method includes: providing a controller to select a positive separation process or a negative separation process to execute according to types of target biosubstances; providing a pipette pump to inject a first magnetic bead reagent or a second magnetic bead reagent into a sample according to the selected separation process, such that first immunomagnetic beads of the first magnetic bead reagent or second immunomagnetic beads of the second magnetic bead reagent are used for binding to the target biosubstances; providing a magnetic separation rack to enrich the target biosubstances so as to separate the target biosubstances and non-target biosubstances; and providing the pipette pump to separate the target biosubstances and the non-target biosubstances.

The present invention relates to compositions comprising magnetic particles, the methods of using these compositions in processing animal sperm, the resulting sperm and embryo products, and the methods of use of these compositions to increase the efficiency, efficacy and/or speed of cell processing and artificial insemination techniques.

A pneumatically-operated magnetic separator captures magnetic contaminants from process fluids. Process fluids are used in many industrial operations for various purposes. The pneumatically-operated magnetic separator can include a housing wall, a first flange plate assembly, a second flange plate assembly, a main fluid passage, tubes, and shuttles. The first and second flange plate assemblies can each include a pair of flange plates. The tubes extend between the first and second flange plate assemblies, and the shuttles are situated in the tubes. Each of the shuttles includes one or more magnets. During use, the shuttles move longitudinally in the tubes in response to pneumatic actuation and de-actuation, per an example. Weldments attach the tubes and the first and second flange plate assemblies together.

An in-line fitment for connection of a filter to a pipe includes first and second fluid-carrying portions and a non-fluid-carrying spacer. Each fluid-carrying portion includes a socket for receiving an open end of a pipe and a connector for connection of the filter. A screw compression fitting is provided on each of the sockets of the first and second fluid-carrying portions for forming a sealed connection with the open ends of the pipe. The socket of the first fluid-carrying portion has a pipe receiving depth greater than that of the socket of the second fluid-carrying portion for enabling movement of the fitment parallel to the pipe when engaged with one of the open ends of the pipe. The sockets of the first and second fluid-carrying portions are positioned on a common axis and facing away from each other when the fluid-carrying portions are linked by the spacer.

A method for magnetic cellular manipulation may include contacting a composition with a biological sample to form a mixture. The composition may include a plurality of particles. Each particle in the plurality of particles may include a magnetic substrate. The magnetic substrate may be characterized by a magnetic susceptibility greater than zero. The composition may also include a chargeable silicon-containing compound. The chargeable silicon-containing compound may coat at least a portion of the magnetic substrate. The biological sample may include cells and/or cellular structures. The method may also include applying a magnetic field to the mixture to manipulate the composition.

A rack for holding samples and various reagents, wherein the rack may be used for loading the samples and reagents prior to using the reagents. The rack accepts complementary reagent holders, each of which contain a set of reagents for carrying out a predetermined processing operation, such as preparing biological samples for amplifying and detecting polynucleotides extracted from the samples.

Described are various embodiments of methods, devices, systems and kits for magnetic levitation-based separation of mixtures or populations of particles that include various types of particles. Some embodiments of such methods, devices, systems and kits are useful for magnetic levitation-based separation of mixtures or populations of cells that include various cell types. Some other embodiments of the described methods, devices, systems and kits are useful for magnetic levitation-based separation of mixtures or population of cellular or mixtures or population of biological molecules.

Provided herein are apparatuses and systems for mixing liquids and suspensions that include vessels with structures that improve mixing while not contacting liquid delivery components. The apparatuses and systems can include a motor drive that allows speed and directional control of rotation of the vessel. The apparatuses and systems can include one or more magnets for separating magnetic beads in a suspension. Also provided are methods using said apparatuses and systems for mixing and separation processes.