A separation container for extracting a portion of a sample for use or testing and method for preparing samples for downstream use or testing are provided. The separation container may include a body defining an internal chamber. The body may define an opening, and the body may be configured to receive the sample within the internal chamber. The separation container may further include a seal disposed across the opening, such that the seal may be configured to seal the opening of the body, and a plunger movably disposed at least partially inside the internal chamber. The plunger may be configured to be actuated to open the seal and express the portion of the sample.

Described herein are methods, systems and apparatus for separating components of a biological sample; as well as methods of using compositions prepared by same.

Multi-component separation devices configured to separate components of a liquid sample by centrifugation are provided. Aspects of the separation devices include a container having a distal end and a proximal end and a buoy configured to be displaced along a longitudinal axis within the container where the buoy includes one or more sealed chambers. Also provided are methods of using the subject devices to separate components of a multi-component liquid sample such as whole blood, bone marrow aspirate or stromal vascular fraction as well as systems suitable for practicing the subject methods.

A separation container for extracting a portion of a sample for use or testing and method for preparing samples for downstream use or testing are provided. The separation container may include a body defining an internal chamber. The body may define an opening, and the body may be configured to receive the sample within the internal chamber. The separation container may further include a seal disposed across the opening, such that the seal may be configured to seal the opening of the body, and a plunger movably disposed at least partially inside the internal chamber. The plunger may be configured to be actuated to open the seal and express the portion of the sample.

Provided herein are devices and methods for sample isolation comprising a planar member configured to rotate around a bearing, and a plurality of apertures positioned at an angle to the planar member. Exemplary embodiments relate generally to the field of sample isolation, and more particularly to sample isolation using centrifugal and magnetic forces.

A centrifuge including a rotor having at least one recess, at least one protrusion or a combination of the at least one recess or the at least one protrusion defining a concave arrangement, each of the at least one recess, the at least one protrusion or the combination of the at least one recess or the at least one protrusion adapted to receive a corresponding specimen container.

A portable centrifuge comprising a base, which includes a rotational mechanism contained within. A sequester wheel having a plurality of concentric rings, forming at least one channel and a central container. The at least one channel comprising a second volume and the central container comprising a first volume. A first cover is disposed over the sequester wheel to create a seal. A second cover is disposed over the first cover and is rotatable to engage with the rotational mechanism to initiate rotation at a predetermined RPM. The whole blood and/or other tissue is separated into its constituent components and deposited into the at least one channel and the central container. Specific volumes of the constituent components may be calculated to match or substantially match the first and second volumes for predetermined volume control and ease of access.

A separation container for extracting a portion of a sample for use or testing and method for preparing samples for downstream use or testing are provided. The separation container may include a body defining an internal chamber. The body may define an opening, and the body may be configured to receive the sample within the internal chamber. The separation container may further include a seal disposed across the opening, such that the seal may be configured to seal the opening of the body, and a plunger movably disposed at least partially inside the internal chamber. The plunger may be configured to be actuated to open the seal and express the portion of the sample.

A separation container for extracting a portion of a sample for use or testing and method for preparing samples for downstream use or testing are provided. The separation container may include a body defining an internal chamber. The body may define an opening, and the body may be configured to receive the sample within the internal chamber. The separation container may further include a seal disposed across the opening, such that the seal may be configured to seal the opening of the body, and a plunger movably disposed at least partially inside the internal chamber. The plunger may be configured to be actuated to open the seal and express the portion of the sample.

Multi-component separation devices configured to separate components of a liquid sample by centrifugation are provided. Aspects of the separation devices include a container having a distal end and a proximal end and a buoy configured to be displaced along a longitudinal axis within the container where the buoy includes one or more sealed chambers. Also provided are methods of using the subject devices to separate components of a multi-component liquid sample such as whole blood, bone marrow aspirate or stromal vascular fraction as well as systems suitable for practicing the subject methods.