An apparatus for separating cell suspension material into centrate and concentrate, includes a single use structure (178, 240, 250, 370, 414) releasably positioned in a cavity in a solid wall rotatable centrifuge bowl (172). The bowl and portions of single use structure rotate about an axis (174, 428). A stationary inlet feed tube (184, 430), a centrate discharge tube (212, 436) and a concentrate discharge tube (230,448) extend along the axis of the rotating single use structure. A centrate centripetal pump (208, 438) is in fluid connection with the centrate discharge tube. A concentrate centripetal pump (216, 450) is in fluid connection with the concentrate discharge tube. At least one concentrate channel (380, 454) and a concentrate centripetal pump chamber (376,452) have configurations in the structure that facilitate the flow of cell concentrate.

A blood washing system (20) having a rotor (22) defining an internal chamber for receiving a multi-component fluid and a skimmer assembly (24) including a moveable buoy (28) having an orifice (32) fluidly connected to an access port for the rotor for selectively withdrawing separated fractions of the multi-component fluid. The multi-component fluid can be fed into the internal chamber before the rotor (22) can be rotated at a first speed to fractionate the multi-component fluid. A brake can be applied to the rotor to slow rotation of the rotor to a slower second speed or stop rotation of the rotor causing the solid and denser fluid fractions to settle on the bottom wall (44) of the rotor (22). The buoy (28) can have a specific gravity corresponding to a selected fraction such that the buoy floats on a surface of the selected fraction, wherein the fractions floating on the selected fraction can be withdrawn through the orifice (32).

Fluid separation chambers are provided for rotation about an axis in a fluid processing system. The fluid separation chamber may be provided with first and second stages, with the first and second stages being positioned at different axial locations. In another embodiment, at least one of the stages may be provided with a non-uniform outer diameter about the rotational axis, which may define a generally spiral-shaped profile or a different profile for fractionating a fluid or fluid component. One or more of the stages may also have a varying outer diameter along the axis. The profile of the chamber may be provided by the chamber itself (in the case of rigid chambers) or by an associated fixture or centrifuge apparatus (in the case of flexible chambers).

A centrifuge rotor having a curved shape is offset on a spinning rotor base and creates contiguous areas of low to high centrifugal force depending on the distances from the axis of the rotor base and a method of separating components in a fluid based upon a difference in density of the components, the method comprising the steps of providing to a rotor as described herein the fluid containing the mixed together components to be separated based upon the difference in density of the mixed together components; continuously flowing the components in the fluid to the rotor through an input tube connected to the input port while the rotor is spinning about a centrifugal axis of rotation; separating the components in the fluid into fractions based upon the difference in density of the mixed together components with the use of centrifugal force when the rotor is spinning; collecting components having i) a first density via a first tube connected to the output port at the first end on the rotor, ii) a second density via a second tube connected to the output port at the second end on the rotor, iii) a third density via a third tube connected to the output port at the junction on the rotor and iv) a fourth density via a fourth tube connected to the output port between the input port and the output port at the first end.

Provided is a centrifugal field-flow fractionation device in which a liquid sample is less likely to leak from a channel and attachment and detachment work of a channel member is facilitated. By integrally forming an outer peripheral surface 162 and an inner peripheral surface 163 of a channel member 16, the channel member 16 is configured as one hollow member having a channel 161 formed inside. Thus, pressure resistance performance of the channel member 16 is improved, formation of a gap in the channel 161 can be prevented, and deterioration in sealing performance due to secular change is not generated. Accordingly, a liquid sample is less likely to leak from the channel 161. Further, since the channel member 16 can be handled as one member, attachment and detachment work of the channel member 16 is facilitated.

Fluid separation chambers are provided for rotation about an axis in a fluid processing system. The fluid separation chamber may be provided with first and second stages, with the first and second stages being positioned at different axial locations. In another embodiment, at least one of the stages may be provided with a non-uniform outer diameter about the rotational axis, which may define a generally spiral-shaped profile or a different profile for fractionating a fluid or fluid component. One or more of the stages may also have a varying outer diameter along the axis. The profile of the chamber may be provided by the chamber itself (in the case of rigid chambers) or by an associated fixture or centrifuge apparatus (in the case of flexible chambers).

Described are embodiments that include methods and devices for detecting disposables that may be used in medical devices. Embodiments involve the detection of disposables used in apheresis machines. The disposable are configured to fit into portions, or features, of the apheresis machines in predetermined ways and embodiments provide for detecting whether the disposable have been loaded correctly.

A centrifugal field-flow fractionation device includes an annular rotor, an arc-shaped channel member, a rotation drive unit, and a restriction unit. A channel member 16 is provided along an inner peripheral surface of the rotor, has therein a channel 161 for a liquid sample by laminating a plurality of layers, and has an inlet for the liquid sample to the channel 161 and an outlet for the liquid sample from the channel 161. By rotating the rotor, particles in the liquid sample in the channel 161 are classified by centrifugal force. A restriction spacer 64 restricts the channel 161 from being compressed to a height less than a certain height when the channel member 16 is compressed and deformed in a laminating direction.

A centrifugal field-flow fractionation device 1 includes a rotation unit 10, a rotation sensor 41, a first vibration sensor 51, a second vibration sensor 52, and an imbalance amount calculation unit 312. When an imbalance occurs in the rotation unit 10, the imbalance amount calculation unit 312 calculates the imbalance amount, based on a detection signal from the rotation sensor 41, a detection signal from the first vibration sensor 51, and a detection signal from the second vibration sensor 52. That is, when an imbalance occurs in the rotation unit 10, the imbalance is calculated by the configuration in the centrifugal field-flow fractionation device 1.

A centrifugal field-flow fractionation device capable of improving analysis performance and shortening analysis time is provided. A first channel 111 communicating with a channel member is formed on a rotational shaft 11 that rotates together with a rotor. A second channel 644 communicating with the first channel 111 is formed on a fixing portion 60 fixed in a state of facing the rotational shaft 11 along a rotational axis L. A mechanical seal 66 having a pair of seal rings 661 and 662 that come into contact with each other and a biasing member 663 is provided to attach one seal ring 661 to the rotational shaft 11 and the other seal ring 662 to the fixing portion 60. The biasing member 663 biases the pair of seal rings 661 and 662 in a direction in which the pair of seal rings come in contact with each other. Since the rotational shaft 11 can be rotated at a high speed and the liquid sample can be fed at a high pressure, the analysis performance can be improved and the analysis time can be shortened.