An apparatus for separating cell suspension material into centrate and concentrate, includes a single use structure (178, 240, 250) releasably positioned in a cavity in a solid wall rotatable centrifuge bowl (172). The bowl and portions of single use structure rotate about an axis (174). A stationary inlet feed tube (184), a centrate discharge tube (212) and a concentrate discharge tube (230) extend along the axis of the rotating single use structure. A centrate centripetal pump (208) is in fluid connection with the centrate discharge tube. A concentrate centripetal pump (216) is in fluid connection with the concentrate discharge tube. A controller (274) operates responsive to sensors (264, 270) in respective centrate and concentrate discharge lines (262, 268), to control flow rates of a concentrate pump (272) and a centrate pump (266) to produce output flows of cell concentrate and generally cell free centrate.

A continuous flow centrifuge bowl includes a rotatable outer body, and a top and bottom core that are rotatable with the outer body. The bottom core has a wall extending proximally from a bottom wall. The proximally extending wall is radially outward from at least a portion of the top core and, together with the top core, defines a primary separation region in which initial separation of the whole blood occurs. The bowl may also have a secondary separation region located between the top core and the outer body, and a rotary seal that couples an inlet port and two outlet ports to the outer body. The inlet port may be connected to an inlet tube that extends distally into a whole blood introduction region. Additionally, one of the outlet ports may be connected to an extraction tube that extends into a region below the bottom core.

The invention relates to a system, comprising: a) a sample processing unit, comprising an input port and an output port coupled to a rotating container having at least one sample chamber, the sample processing unit configured provide a first processing step to a sample or to rotate the container so as to apply a centrifugal force to a sample deposited in the chamber and separate at least a first component and a second component of the deposited sample; and b) a sample separation unit coupled to the output port of the sample processing unit, the cell separation unit comprising separation column holder, a pump and a plurality of valves configured to at least partially control fluid flow through a fluid circuitry and a separation column positioned in the holder, the separation column configured to separate labeled and unlabeled components of sample flowed through the column.

A continuous flow centrifuge bowl includes a rotatable outer body, and a top and bottom core that are rotatable with the outer body. The bottom core has a wall extending proximally from a bottom wall. The proximally extending wall is radially outward from at least a portion of the top core and, together with the top core, defines a primary separation region in which initial separation of the whole blood occurs. The bowl may also have a secondary separation region located between the top core and the outer body, and a rotary seal that couples an inlet port and two outlet ports to the outer body. The inlet port may be connected to an inlet tube that extends distally into a whole blood introduction region. Additionally, one of the outlet ports may be connected to an extraction tube that extends into a region below the bottom core.

A system for removing undesirable elements from blood. The system includes a centrifuge bowl to separate the blood into components according to relative densities of the components, a pump to provide wash solution that washes the blood in the centrifuge bowl, and a controller to wash the blood in the centrifuge bowl in a first wash and remove first undesirable elements and to wash the blood in the centrifuge bowl in a second wash and remove trapped undesirable elements. The controller to further mix the blood and the wash solution in the centrifuge bowl and provide diluted blood, separate the diluted blood into concentrated blood and the wash solution, fill the centrifuge bowl with previously concentrated blood to build a buffy coat, and empty the centrifuge bowl of the concentrated blood and the previously concentrated blood after the buffy coat is reached.

A continuous flow centrifuge bowl includes a rotatable outer body, and a top and bottom core that are rotatable with the outer body. The bottom core has a wall extending proximally from a bottom wall. The proximally extending wall is radially outward from at least a portion of the top core and, together with the top core, defines a primary separation region in which initial separation of the whole blood occurs. The bowl may also have a secondary separation region located between the top core and the outer body, and a rotary seal that couples an inlet port and two outlet ports to the outer body. The inlet port may be connected to an inlet tube that extends distally into a whole blood introduction region. Additionally, one of the outlet ports may be connected to an extraction tube that extends into a region below the bottom core.

Provided is a chamber configuration for a reverse flow centrifuge, and a reverse flow centrifuge system configured for low fluid volume and small radius rotation. The compact reverse flow centrifuge system has a reusable subsystem and a single use replaceable subsystem. The replaceable subsystem comprises a separation chamber, fluid delivery manifold and rotational mounting connecting the separation chamber to the fluid manifold. The single use replaceable subsystem provides a closed environment for execution of reverse flow centrifugation processes. The separation chamber has a substantially conical fluid enclosure portion connected to a neck portion, and a dip tube extends centrally through the conical fluid enclosure to provide a fluid path to the tip of the conical fluid enclosure.

A system for removing undesirable elements from blood. The system includes a centrifuge bowl to separate the blood into components according to relative densities of the components, a pump to provide wash solution that washes the blood in the centrifuge bowl, and a controller to wash the blood in the centrifuge bowl in a first wash and remove first undesirable elements and to wash the blood in the centrifuge bowl in a second wash and remove trapped undesirable elements. The controller to further mix the blood and the wash solution in the centrifuge bowl and provide diluted blood, separate the diluted blood into concentrated blood and the wash solution, fill the centrifuge bowl with previously concentrated blood to build a buffy coat, and empty the centrifuge bowl of the concentrated blood and the previously concentrated blood after the buffy coat is reached.

An apparatus for separating cell suspension material into centrate and concentrate, includes a single use structure (178, 240, 250, 370, 414) releasably positioned in a cavity in a solid wall rotatable centrifuge bowl (172). The bowl and portions of single use structure rotate about an axis (174, 428). A stationary inlet feed tube (184, 430), a centrate discharge tube (212, 436) and a concentrate discharge tube (230,448) extend along the axis of the rotating single use structure. A centrate centripetal pump (208, 438) is in fluid connection with the centrate discharge tube. A concentrate centripetal pump (216, 450) is in fluid connection with the concentrate discharge tube. At least one concentrate channel (380, 454) and a concentrate centripetal pump chamber (376,452) have configurations in the structure that facilitate the flow of cell concentrate.

A centrifuge bowl for separating whole blood into blood components includes a rotatable body, and inlet, and a plurality of vibration reduction members. The rotatable body has a body portion and a neck portion. The body portion defines an interior for receiving whole blood, and the body is rotatable to separate the whole blood into a plurality of blood components. The inlet is in fluid communication with the interior of the rotatable body, and is configured to introduce the whole blood into the rotatable body. The plurality of vibration reduction members are spaced about the neck portion, and are configured to reduce vibration of the centrifuge bowl as the bowl is rotated.