A centrifugal separator bowl, having a vertical rotational axis, the separator bowl defining a separation space, and including an upper bowl section having a first threading, the first threading having a first inner diameter and a first outer diameter and defining an upper surface, and a lower bowl section having a second threading corresponding to the first threading of the upper bowl section, the second threading having a second outer diameter and a second inner diameter and defining a lower surface. The upper surface of the first threading of the upper bowl section and the lower surface of the second threading of the lower bowl section form a screw connection. The upper surface of the first threading is inclined upward with a first angle from the first inner diameter to the first outer diameter and the lower surface of the second threading is inclined downward with a second angle from the second outer diameter to the second inner diameter relative to a horizontal plane extending perpendicular to the vertical rotation axis.

A centrifugal separator bowl, having a vertical rotational axis, the separator bowl defining a separation space, and including an upper bowl section having a first threading, the first threading having a first inner diameter and a first outer diameter and defining an upper surface, and a lower bowl section having a second threading corresponding to the first threading of the upper bowl section, the second threading having a second outer diameter and a second inner diameter and defining a lower surface. The upper surface of the first threading of the upper bowl section and the lower surface of the second threading of the lower bowl section form a screw connection. The upper surface of the first threading is inclined upward with a first angle from the first inner diameter to the first outer diameter and the lower surface of the second threading is inclined downward with a second angle from the second outer diameter to the second inner diameter relative to a horizontal plane extending perpendicular to the vertical rotation axis.

A method for separating a cell culture mixture utilizes a centrifugal separator including a stationary frame, a rotatable assembly and a drive unit for rotating the rotatable assembly relative the frame around a vertical axis of rotation. The rotatable assembly includes a rotor casing enclosing a separation space in which a stack of separation discs is arranged to rotate around an axis of rotation. The rotor casing includes a mechanically hermetically sealed inlet for supply of the cell culture mixture to the separation space and first and second mechanically hermetically sealed liquid outlets. The second mechanically hermetically sealed outlet is arranged at an axial end of the rotor casing and arranged so that the separated cell phase is discharged at the rotational axis.

A method for separating a cell culture mixture utilizes a centrifugal separator including a stationary frame, a rotatable assembly and a drive unit for rotating the rotatable assembly relative the frame around a vertical axis of rotation. The rotatable assembly includes a rotor casing enclosing a separation space in which a stack of separation discs is arranged to rotate around an axis of rotation. The rotor casing includes a mechanically hermetically sealed inlet for supply of the cell culture mixture to the separation space and first and second mechanically hermetically sealed liquid outlets. The second mechanically hermetically sealed outlet is arranged at an axial end of the rotor casing and arranged so that the separated cell phase is discharged at the rotational axis.

A centrifugal separation system includes a centrifugal separator, a liquid feed mixture conduit, a light phase conduit, a heavy phase conduit, and a flow control system. The flow control system includes a controller, a counterpressure generating arrangement connected to a heavy phase conduit, a liquid feed mixture measuring device, and a light phase measuring device and/or a heavy phase measuring device. The counterpressure generating arrangement includes a heavy phase receiving vessel and a heavy phase pressure control arrangement connected to the vessel. The controller is configured to control the heavy phase pressure control arrangement based on measurements from the liquid feed mixture measuring device and on measurements from the light phase measuring device and/or the heavy phase measuring device in order to control a heavy phase counterpressure in the heavy phase outlet passage.

An exchangeable separation insert for a centrifugal separator includes a feed inlet, a light phase outlet and a heavy phase outlet. Two of the feed inlet, light phase outlet and heavy phase outlet are arranged at a first axial end of the rotor casing. A first seal assembly seals and connects the two inlet/outlets to a corresponding inlet conduit and/or outlet conduits in the first stationary portion. The first seal assembly includes a rotatable part and a stationary part. The rotatable and stationary part are axially aligned and seal against each other. A first of the two inlet/outlets is arranged axially at the axis of rotation and a second of the two inlet/outlets is arranged axially outside of the first of the two inlet/outlets in such a manner that both the first and second of the two inlet/outlets are led through the rotatable part and the corresponding inlet conduit and/or outlet conduits are led through the stationary part of the first seal assembly.

An exchangeable separation insert for a centrifugal separator includes a feed inlet, a light phase outlet and a heavy phase outlet. Two of the feed inlet, light phase outlet and heavy phase outlet are arranged at a first axial end of the rotor casing. A first seal assembly seals and connects the two inlet/outlets to a corresponding inlet conduit and/or outlet conduits in the first stationary portion. The first seal assembly includes a rotatable part and a stationary part. The rotatable and stationary part are axially aligned and seal against each other. A first of the two inlet/outlets is arranged axially at the axis of rotation and a second of the two inlet/outlets is arranged axially outside of the first of the two inlet/outlets in such a manner that both the first and second of the two inlet/outlets are led through the rotatable part and the corresponding inlet conduit and/or outlet conduits are led through the stationary part of the first seal assembly.

A separation disc for a centrifugal separator is adapted to be included in a stack of separation discs inside a centrifugal rotor for separating a fluid mixture. The separation disc has a truncated conical shape with an inner surface and an outer surface and a plurality of spot-formed spacing members extending from at least one of the inner surface and the outer surface. The spot-formed spacing members are for providing interspaces between mutually adjacent separation discs in a stack of separation discs, and the plurality of spot-formed spacing members are tip-shaped and taper from a base at the surface of the separation disc towards a tip extending a height from the surface. A stack of separation discs, a centrifugal separator and a method for separating at least two components of a fluid mixture are also disclosed.

A separation disc for a centrifugal separator is adapted to be included in a stack of separation discs inside a centrifugal rotor for separating a fluid mixture. The separation disc has a truncated conical shape with an inner surface and an outer surface and a plurality of spot-formed spacing members extending from at least one of the inner surface and the outer surface. The spot-formed spacing members are for providing interspaces between mutually adjacent separation discs in a stack of separation discs, and the plurality of spot-formed spacing members are tip-shaped and taper from a base at the surface of the separation disc towards a tip extending a height from the surface. A stack of separation discs, a centrifugal separator and a method for separating at least two components of a fluid mixture are also disclosed.

A centrifugal separator (14) comprises a stationary frame (15), a spindle (16) rotatably supported by the frame, a centrifuge rotor 17 mounted to a first end of the spindle to rotate together with the spindle around an axis (X2) of rotation. A stack (10) of separation discs (1) is arranged to rotate coaxially with the centrifuge rotor The stack of separation discs comprises a set of axially aligned separation discs with an inner surface and an outer surface and a plurality spot-formed spacing members extending from a base from at least one of the inner surface and the outer surface The surface density of said spot-formed spacing members is above 25 spot-formed spacing members/dm.sup.2 throughout the inner and/or outer surface of the separation disc. A first region at a bottom of the discs has a higher density of the spot-formed spacing members than a second region.