A method of preparing hair follicle-derived conditioned medium which comprises one or more bioactive factors, wherein the hair follicle is retrieved from a subject non-invasively, cultured in a culture medium for a duration sufficient for a hair follicle-derived cell to secrete the one or more bioactive factors to condition the medium. A hair follicle-derived conditioned medium comprising one or more bioactive factors secreted by a hair follicle-derived cell retrieved from a subject non-invasively. A composition comprising said conditioned medium. Use of said conditioned medium and composition for therapeutic or cosmetic treatment.