A method for printing uses at least one bio-ink. The method also uses at least one laser print head to deposit at least one droplet of at least one bio-ink onto a depositing surface of a receiving substrate. The printing method uses at least one nozzle print head to deposit at least one droplet of at least one bio-ink onto a depositing surface of the same receiving substrate as the laser print head.

Methods of bioprinting a bio-ink construct on an internal tissue defect or a chondral defect during a minimally invasive surgery on an individual in need thereof are provided, comprising: visualizing the defect; positioning a bioprinter comprising a printhead within proximity of or in contact with the defect; and ejecting a bio-ink from the printhead onto the defect to form a bio-ink layer, thereby generating a bio-ink construct. Further provided are systems for bioprinting a bio-ink construct on an internal tissue defect during a minimally invasive surgery on an individual in need thereof, comprising a control system, an endoscope, and a bioprinter comprising a printhead.

Methods of bioprinting a bio-ink construct on an internal tissue defect or a chondral defect during a minimally invasive surgery on an individual in need thereof are provided, comprising: visualizing the defect; positioning a bioprinter comprising a printhead within proximity of or in contact with the defect; and ejecting a bio-ink from the printhead onto the defect to form a bio-ink layer, thereby generating a bio-ink construct. Further provided are systems for bioprinting a bio-ink construct on an internal tissue defect during a minimally invasive surgery on an individual in need thereof, comprising a control system, an endoscope, and a bioprinter comprising a printhead.

Methods and systems are disclosed for selecting a print head of a 3D printing machine to apply 3D printing material at one or more positions in a printed 3D object. Embodiments of the present invention may include, in the selection process one or more elements pertaining for example to: geometry of the received 3D model; colorization of the received 3D model; transparency of the received 3D model; properties of the 3D printing material; and constraints of the 3D printing machine.

Methods and systems are disclosed for selecting a print head of a 3D printing machine to apply 3D printing material at one or more positions in a printed 3D object. Embodiments of the present invention may include, in the selection process one or more elements pertaining for example to: geometry of the received 3D model; colorization of the received 3D model; transparency of the received 3D model; properties of the 3D printing material; and constraints of the 3D printing machine.

A method of layerwise fabrication of a three-dimensional object is disclosed. The method comprises, for each of at least a few of the layers: dispensing at least a first modeling formulation and a second modeling formulation to form a core region using both the first and the second modeling formulations, and at least one envelope region at least partially surrounding the core region using one of the first and the second modeling formulations but not the other one of the first and the second modeling formulations. The method can also comprise exposing the layer to curing energy. The first modeling formulation is characterized, when hardened, by heat deflection temperature (HDT) of at least 90° C., and the second modeling formulation is characterized, when hardened, by Izod impact resistance (IR) value of at least 45 J/m.

A method of layerwise fabrication of a three-dimensional object is disclosed. The method comprises, for each of at least a few of the layers: dispensing at least a first modeling formulation and a second modeling formulation to form a core region using both the first and the second modeling formulations, and at least one envelope region at least partially surrounding the core region using one of the first and the second modeling formulations but not the other one of the first and the second modeling formulations. The method can also comprise exposing the layer to curing energy. The first modeling formulation is characterized, when hardened, by heat deflection temperature (HDT) of at least 90° C., and the second modeling formulation is characterized, when hardened, by Izod impact resistance (IR) value of at least 45 J/m.

A raster image conversion device is inputted with printing data and converts this printing data into raster image data to be outputted to a printing device. A storage unit stores printing environment data stipulating conditions for converting the printing data into the raster image data. An operating unit sets or edits the printing environment data. A conversion processing unit converts the printing data into the raster image data, based on the printing environment data. The printing environment data includes printing template information having a plurality of printing slots. The operating unit comprises an operating mode enabling the printing template information to be generated or edited. The conversion processing unit is inputted with or creates printing data made up of a plurality of pages, and, based on the printing template information, allocates a printing position and printing range of each page of the printing data to the printing slots.

The present disclosure describes multi-fluid kits for three-dimensional printing, three-dimensional printing kits, and methods of making three-dimensional printed objects. In one example, a multi-fluid kit for three-dimensional printing can include a fusing agent and a tinted anti-coalescing agent. The fusing agent can include water and an electromagnetic radiation absorber that absorbs radiation energy and converts the radiation energy to heat. The tinted anti-coalescing agent can include an aqueous liquid vehicle, a colored dye dissolved in the aqueous liquid vehicle, and an organosilane. The organosilane can have a central silicon atom covalently coupled to multiple hydrolysable groups and to an organic group by covalent bonding to a carbon atom in the organic group, wherein the organic group is not susceptible to hydrolysis.

The present disclosure describes multi-fluid kits for three-dimensional printing, three-dimensional printing kits, and methods of making three-dimensional printed objects. In one example, a multi-fluid kit for three-dimensional printing can include a fusing agent and a tinted anti-coalescing agent. The fusing agent can include water and an electromagnetic radiation absorber that absorbs radiation energy and converts the radiation energy to heat. The tinted anti-coalescing agent can include an aqueous liquid vehicle, a colored dye dissolved in the aqueous liquid vehicle, and an organosilane. The organosilane can have a central silicon atom covalently coupled to multiple hydrolysable groups and to an organic group by covalent bonding to a carbon atom in the organic group, wherein the organic group is not susceptible to hydrolysis.