The invention relates to methods of cultivating ectomycorrhizal fungi, the method comprising incubating ectomycorrhizal MAT1-1 mycelia, ectomycorrhizal MAT1-2 mycelia, and ectomycorrhizal male mycelia in a culture medium for about 1 day to about 50 days, thereby cultivating ectomycorrhizal fungi to produce an ectomycorrhizal fungal biomass comprising MAT1-1 ectomycorrhizal mycelia, MAT1-2 ectomycorrhizal mycelia, and male ectomycorrhizal mycelia. Also provided are methods of use of the ectomycorrhizal mycelial biomass.