Provided are a straw returning method through thawing and wet puddling for saline-sodic paddy fields and an application thereof, relating to the technical field of returning straws to fields in saline-sodic paddy fields. According to the disclosure, regional characteristics are fully considered, thawing is taken as the core and combined with wet puddling.

The present invention discloses an endophytic fungus FO-R20 and applications thereof, and belongs to the technical filed of microbial applications. The deposit number of the endophytic fungus FO-R20 is CCTCC M 2021505, and the scientific name thereof is Falciphora oryzae. The present invention provides a novel endophytic fungal strain in the genus Phialophora. Colonization of the endophytic fungus FO-R20 in the root tissues of rice can significantly improve the quality of rice seedlings and enhance the rice yields. The remarkable effect of symbiosis and interactions of the endophytic fungus FO-R20 on rice has huge value in the agricultural field by application and popularization thereof.

The present invention discloses an endophytic fungus FO-R20 and applications thereof, and belongs to the technical filed of microbial applications. The deposit number of the endophytic fungus FO-R20 is CCTCC M 2021505, and the scientific name thereof is Falciphora oryzae. The present invention provides a novel endophytic fungal strain in the genus Phialophora. Colonization of the endophytic fungus FO-R20 in the root tissues of rice can significantly improve the quality of rice seedlings and enhance the rice yields. The remarkable effect of symbiosis and interactions of the endophytic fungus FO-R20 on rice has huge value in the agricultural field by application and popularization thereof.

The invention discloses gene sequences of a plant EPSPS mutant and its application in plants. As compared to the wild type, the present disclosure describes a plant EPSP synthase (EPSPS) polypeptide which comprises amino acid V mutating to amino acid A corresponding to the location at V403 or the analogous location at V403, wherein the amino acid position corresponds to the amino acid position set forth in SEQ ID NO:1.

Disclosed is a rice and wheat nitrogen nutrition multispectral diagnosis method for precise fertilization by unmanned aerial vehicles (UAVs), including: step 1, acquiring multispectral UAV remote sensing images and collecting agronomic parameters of rice and wheat, and establishing a nitrogen nutrition spectral diagnosis model; and step 2, calculating a critical nitrogen concentration in aboveground parts of rice and wheat based on a critical nitrogen concentration dilution model for amount of dry matter, and further obtaining a critical nitrogen uptake; calculating an actual nitrogen uptake in the aboveground parts of rice and wheat based on the nitrogen nutrition spectral diagnosis model; and obtaining a recommended nitrogen topdressing amount for rice and wheat during corresponding stages based on the critical nitrogen uptake and the actual nitrogen uptake. The present disclosure ensures stable yields of rice and wheat while reducing input of chemical fertilizers and labor, presenting a promising application prospect.

Disclosed is a rice and wheat nitrogen nutrition multispectral diagnosis method for precise fertilization by unmanned aerial vehicles (UAVs), including: step 1, acquiring multispectral UAV remote sensing images and collecting agronomic parameters of rice and wheat, and establishing a nitrogen nutrition spectral diagnosis model; and step 2, calculating a critical nitrogen concentration in aboveground parts of rice and wheat based on a critical nitrogen concentration dilution model for amount of dry matter, and further obtaining a critical nitrogen uptake; calculating an actual nitrogen uptake in the aboveground parts of rice and wheat based on the nitrogen nutrition spectral diagnosis model; and obtaining a recommended nitrogen topdressing amount for rice and wheat during corresponding stages based on the critical nitrogen uptake and the actual nitrogen uptake. The present disclosure ensures stable yields of rice and wheat while reducing input of chemical fertilizers and labor, presenting a promising application prospect.

A method for cultivating functional crops using nano organic germanium and nano organic selenium, includes performing irrigation or foliar fertilization on plants of the crops with nanoscaled organic germanium and nanoscaled organic selenium, which are prepared in nanoscale sizes by performing one or two or more selected from a method of applying physical energy (heat or pressure) to organic germanium and organic selenium, a method of applying electrical explosive energy, and a chemical bonding process, at least twice at the time when the plants of the crops are most vigorously grown and developed and at the time of maturation to bear fruit, wherein the prepared nanoscaled organic germanium and nanoscaled organic selenium are one-component materials which are dispersed in water as a solvent in a nanoscale state.

A method for cultivating functional crops using nano organic germanium and nano organic selenium, includes performing irrigation or foliar fertilization on plants of the crops with nanoscaled organic germanium and nanoscaled organic selenium, which are prepared in nanoscale sizes by performing one or two or more selected from a method of applying physical energy (heat or pressure) to organic germanium and organic selenium, a method of applying electrical explosive energy, and a chemical bonding process, at least twice at the time when the plants of the crops are most vigorously grown and developed and at the time of maturation to bear fruit, wherein the prepared nanoscaled organic germanium and nanoscaled organic selenium are one-component materials which are dispersed in water as a solvent in a nanoscale state.

Disclosed is a rice and wheat nitrogen nutrition multispectral diagnosis method for precise fertilization by unmanned aerial vehicles (UAVs), including: step 1, acquiring multispectral UAV remote sensing images and collecting agronomic parameters of rice and wheat, and establishing a nitrogen nutrition spectral diagnosis model; and step 2, calculating a critical nitrogen concentration in aboveground parts of rice and wheat based on a critical nitrogen concentration dilution model for amount of dry matter, and further obtaining a critical nitrogen uptake; calculating an actual nitrogen uptake in the aboveground parts of rice and wheat based on the nitrogen nutrition spectral diagnosis model; and obtaining a recommended nitrogen topdressing amount for rice and wheat during corresponding stages based on the critical nitrogen uptake and the actual nitrogen uptake. The present disclosure ensures stable yields of rice and wheat while reducing input of chemical fertilizers and labor, presenting a promising application prospect.

Disclosed is a rice and wheat nitrogen nutrition multispectral diagnosis method for precise fertilization by unmanned aerial vehicles (UAVs), including: step 1, acquiring multispectral UAV remote sensing images and collecting agronomic parameters of rice and wheat, and establishing a nitrogen nutrition spectral diagnosis model; and step 2, calculating a critical nitrogen concentration in aboveground parts of rice and wheat based on a critical nitrogen concentration dilution model for amount of dry matter, and further obtaining a critical nitrogen uptake; calculating an actual nitrogen uptake in the aboveground parts of rice and wheat based on the nitrogen nutrition spectral diagnosis model; and obtaining a recommended nitrogen topdressing amount for rice and wheat during corresponding stages based on the critical nitrogen uptake and the actual nitrogen uptake. The present disclosure ensures stable yields of rice and wheat while reducing input of chemical fertilizers and labor, presenting a promising application prospect.