The present invention provides methods of preparing plants, with specific predetermined mutation(s) in one or more NOI(s). The specific predetermined mutation(s) preferably may result in the identification of plants having desired traits.

The invention provides novel methods for liquid-mediated delivery of pollen grains to enclosed stigmas in recipient female flowers. For example, methods for liquid-mediated pollination are provided. The methods provided include collecting pollen from a donor plant, suspending the collected pollen in a liquid solution, and introducing said solution to an enclosed stigma of a recipient flower bud on a recipient plant, thereby pollinating the flower with the pollen from the donor plant.

The present invention provides methods of preparing plants, with specific predetermined mutation(s) in one or more NOI(s). The specific predetermined mutation(s) preferably may result in the identification of plants having desired traits.

The present disclosure relates to methods of producing viable Cannabis sativa seeds by inducing male flowers on a genetically female Cannabis sativa plant. The pollen produced by the induced flowers of such plant can be used to pollinate female flowers from the same plant or another female plant to produce seed. In certain embodiments, feminized Cannabis sativa seed is produced on a production scale.

The present invention provides pollen compositions, methods and kits for making said compositions, and methods of using said compositions to fertilize a plant. The pollen within these compositions has increased longevity and can be used to fertilize plants for an extended period of time as compared to pollen prepared using conventional methods. Also provided are methods of collecting pollen from a maize plant by maturing individual tassel branches off of the plant and vessels for collecting the pollen from these individual branches.

Plant cell fate and development is altered by treating cells with cellular reprogramming factors. Embryogenesis inducing embryogenesis factor genes and/or morphogenic developmental genes are used as cellular reprogramming factors, specifically comprising polypeptides or polynucleotides encoding gene products for generating doubled haploids or haploid plants from gametes. Maize microspores treated by contacting the isolated cells with an exogenous purified, recombinant embryogenesis inducing embryogenesis factor gene products and/or morphogenic developmental gene polypeptide results in embryogenesis. The gametes of a maize plant develop into embryoids when transformed with a genetic construct including regulatory elements and structural genes capable of acting in a cascading fashion to alter cellular fate of plant cells. Embryogenesis factor proteins and/or developmental morphogenic proteins expressed from a genetic construct are used for ex situ treatment methods and for in planta cellular reprogramming.

Exemplary embodiments described herein include various methods for creating clonal seeds from which cannabis females are generated. Clonal seeds are cannabis seeds that give rise to identical plants. Specifically, clonal seeds may be genetically identical in certain embodiments and almost genetically identical in other embodiments.