The present invention discloses a maternal haploid major inducing gene in maize and application thereof and also discloses a major gene for inducing the production of maize maternal haploid and its application in double haploid (DH) breeding. This gene encodes a phospholipase (PLA) and the nucleotide sequence thereof is SEQ ID NO: 1. This gene has a maternal haploid inducing ability during selfing or the hybridization of the material as a paternal and other maize material, after a mutation occurred in the coding region. The present invention obtains a series of allelic mutations of the gene for the first time, and proves its maternal haploid induction function by selfing and hybridization. The acquisition of the maternal haploid has important theoretical and practical significance for haploid breeding (DH) and related research.

The methods and compositions described herein relate to maintainer lines (e.g, male-fertile lines) for producing or propogation of plants with a male-sterile phenotype.

The methods and compositions described herein relate to maintainer lines (e.g, male-fertile lines) for producing or propogation of plants with a male-sterile phenotype.

Provided herein are materials and in planta methods for using haploid inducer lines containing a targeted endonuclease to generate transgenic or non-transgenic plants with targeted mutations and/or genomic modifications. Also provided herein.

Provided herein are materials and in planta methods for using haploid inducer lines containing a targeted endonuclease to generate transgenic or non-transgenic plants with targeted mutations and/or genomic modifications. Also provided herein.

A method for breeding polyploid two-line hybrid rice includes determining a tetraploid rice photo thermosensitive genic male sterile line with the gene characteristic of PMeS (polyploid meiosis stability) and a tetraploid rice restoring line with the gene characteristic of PMeS; hybridizing and matching by indica sterile/japonica restoring or japonica sterile/indica restoring hybrid combination; preparing a tetraploid rice hybrid by adopting a tetraploid rice photo thermosensitive genic male sterile line and a tetraploid rice restoring line; and breeding a stable tetraploid rice hybrid combination which is determined as the polyploid two-line hybrid rice combination. The breeding method disclosed by the present invention utilizes the strong heterosis of polyploid rice, and transforms the existing diploid heterosis into the heterosis of polyploid two-line hybrid rice; and by adopting the method disclosed by the present invention, a new polyploid two-line hybrid rice variety with large ears, large grains and high yield can be bred.

A method for breeding polyploid two-line hybrid rice includes determining a tetraploid rice photo thermosensitive genic male sterile line with the gene characteristic of PMeS (polyploid meiosis stability) and a tetraploid rice restoring line with the gene characteristic of PMeS; hybridizing and matching by indica sterile/japonica restoring or japonica sterile/indica restoring hybrid combination; preparing a tetraploid rice hybrid by adopting a tetraploid rice photo thermosensitive genic male sterile line and a tetraploid rice restoring line; and breeding a stable tetraploid rice hybrid combination which is determined as the polyploid two-line hybrid rice combination. The breeding method disclosed by the present invention utilizes the strong heterosis of polyploid rice, and transforms the existing diploid heterosis into the heterosis of polyploid two-line hybrid rice; and by adopting the method disclosed by the present invention, a new polyploid two-line hybrid rice variety with large ears, large grains and high yield can be bred.

Methods of creating doubled haploid inducers are provided. These doubled haploid inducers are used in the production of maternal doubled haploids.

Methods of creating doubled haploid inducers are provided. These doubled haploid inducers are used in the production of maternal doubled haploids.

Methods and compositions for modulating plant development are provided. Nucleotide sequences and amino acid sequences encoding Ovule Development Protein 2 (ODP2) proteins are provided. The sequences can be used in a variety of methods including modulating development, developmental pathways, altering oil content in a plant, increasing transformation efficiencies, modulating stress tolerance, and modulating the regenerative capacity of a plant. Transformed plants, plant cells, tissues, and seed are also provided.