Provided here are methods of using a mutated patatin-like phospholipase II (pPLAII, renamed here MATRILINEAL) to induce haploid induction in plants, cloning a pPLAII to induce haploid induction in plants, and genetically engineering a plant to contain a mutated pPLAII. Also provided are methods of applying topical and spray chemicals, lipids, and RNAi molecules to plants during pollination in order to induce haploid production. Further provided are methods of chemically treating plants during pollination to induce haploids while also reducing embryo abortion and increasing seed set.

Provided here are methods of using a mutated patatin-like phospholipase II (pPLAII, renamed here MATRILINEAL) to induce haploid induction in plants, cloning a pPLAII to induce haploid induction in plants, and genetically engineering a plant to contain a mutated pPLAII. Also provided are methods of applying topical and spray chemicals, lipids, and RNAi molecules to plants during pollination in order to induce haploid production. Further provided are methods of chemically treating plants during pollination to induce haploids while also reducing embryo abortion and increasing seed set.

Plants, seeds and tissue cultures of the hybrid rice HR170002, and methods for producing a rice plant by crossing a rice plant of hybrid rice HR170002 with itself or with another rice plant, such as a plant of another rice variety or rice hybrid, are disclosed.

The presently disclosed subject matter relates to using a haploid inducing line (whether existing or created) and transforming the haploid line so that it encodes cellular machinery capable of editing genes. The transformed haploid inducing line is used as a parent in a cross between two plants. During pollination, the parental gametes fuse to form an embryo; and the gene editing machinery is also delivered to the embryo at this time. During embryonic development, one set of parental chromosomes are lost, and the gene editing machinery operates on the remaining set of chromosomes. Thus, at least one haploid progeny with edited genes is produced from the cross.

Sorghum plants are provided which are capable of inducing haploidy by modifications in the genome related to a pollen-specific expressed patatin phospholipid producing haploid offspring and can be produced for hybrid breeding in short time by chromosome doubling inbred lines, that is, homozygous father and mother lines. In addition, methods are provided for producing transgenic and non-transgenic plant haploid inducers and improving the induction performance of plants.

Provided here are methods of using a mutated patatin-like phospholipase II? (pPLAII?, renamed here MATRILINEAL) to induce haploid induction in plants, cloning a pPLAII? to induce haploid induction in plants, and genetically engineering a plant to contain a mutated pPLAII?. Also provided are methods of applying topical and spray chemicals, lipids, and RNAi molecules to plants during pollination in order to induce haploid production. Further provided are methods of chemically treating plants during pollination to induce haploids while also reducing embryo abortion and increasing seed set.

Provided here are methods of using a mutated patatin-like phospholipase II? (pPLAII?, renamed here MATRILINEAL) to induce haploid induction in plants, cloning a pPLAII? to induce haploid induction in plants, and genetically engineering a plant to contain a mutated pPLAII?. Also provided are methods of applying topical and spray chemicals, lipids, and RNAi molecules to plants during pollination in order to induce haploid production. Further provided are methods of chemically treating plants during pollination to induce haploids while also reducing embryo abortion and increasing seed set.

The present specification discloses a method for detecting an aneuploid of a Brassica oleracea plant, including: performing real-time PCR using DNA extracted from a sample derived from a Brassica oleracea plant to be tested as a template and DNA markers specific to each of two or more chromosomes of Brassica oleracea plant; and detecting chromosomal aneuploidy from a relative difference in amplification amount between the obtained DNA markers. According to one embodiment of the present invention, it is possible to simply, accurately, and rapidly detect an off-type (chromosomal aneuploid) that may occur in Brassica oleracea varieties, in a laboratory equipped with a general molecular biological equipment in the course of seed quality control and breeding research.

Provided are methods for increasing the yield of a monocot plant through treatment of the plant with a plant growth regulator. In certain embodiments, maize plants produce multiple ears and an increased number of kernels. In certain embodiments, reduced height of the plant allows for more efficient self-pollination.

Provided are methods for increasing the yield of a monocot plant through treatment of the plant with a plant growth regulator. In certain embodiments, maize plants produce multiple ears and an increased number of kernels. In certain embodiments, reduced height of the plant allows for more efficient self-pollination.