A gene ZmPLD3 for inducing maize maternal haploid production and its application thereof. Transgenic homozygous mutant plants or their progeny can be obtained by knocking out the ZmPLD3 gene in maize, and maize maternal haploids can be produced by hybridizing them as paternal materials with other maize materials. A series of allelic mutations of the gene having maternal haploid induction function through hybridization were obtained. The experiments showed that the mutation of maize phospholipase PLD3 could lead to the production of maize maternal haploid, which provides new thoughts for revealing the biological role of phospholipase in maize maternal haploid induction process. At the same time, the mutant individuals have the maternal haploid induction ability in maize, which is of great significance for breeding new types of haploid induction lines with high haploid induction rate and improving the efficiency of maize haploid breeding.

The invention provides methods for producing seeds in watermelon. In one embodiment methods are provided comprising grafting of a seed parent onto a stress tolerant rootstock, pollinating the seed parent with pollen from a pollen donor, and cultivating the seed parent until seed is formed. In specific embodiments, triploid seeds produced by a method of the invention are rendered conspicuously distinguishable from tetraploid seeds, and thus readily selected manually or by an automated machine. Methods for increasing seed yield and/or quality are also provided.

Plant cell fate and development is altered by treating cells with cellular reprogramming factors. Embryogenesis inducing morphogenic developmental genes are used as cellular reprogramming factors, specifically comprising polypeptides or polynucleotides encoding gene products for generating doubled haploids or haploid plants from gametes. Maize microspores treated by contacting the isolated cells with an exogenous purified, recombinant embryogenesis inducing morphogenic developmental gene polypeptide results in embryogenesis. The gametes of a maize plant develop into embryoids when transformed with a genetic construct including regulatory elements and structural genes capable of acting in a cascading fashion to alter cellular fate of plant cells. Developmental morphogenic proteins expressed from a genetic construct are used for ex situ treatment methods and for in planta cellular reprogramming.

Plant cell fate and development is altered by treating cells with cellular reprogramming factors. Embryogenesis inducing morphogenic developmental genes are used as cellular reprogramming factors, specifically comprising polypeptides or polynucleotides encoding gene products for generating doubled haploids or haploid plants from gametes. Maize microspores treated by contacting the isolated cells with an exogenous purified, recombinant embryogenesis inducing morphogenic developmental gene polypeptide results in embryogenesis. The gametes of a maize plant develop into embryoids when transformed with a genetic construct including regulatory elements and structural genes capable of acting in a cascading fashion to alter cellular fate of plant cells. Developmental morphogenic proteins expressed from a genetic construct are used for ex situ treatment methods and for in planta cellular reprogramming.

The present disclosure provides a method for obtaining adventitious tetraploid buds of Blumea balsamifera, comprising the following steps: selecting a root segment of diploid B. balsamifera as an explant, culturing the root segment in a chromosome doubling inducing medium supplemented with 0.025-0.1 mg/L 1-naphthaleneacetic acid (NAA), 1.0-2.0 mg/L 6-benzylaminopurine (6-BA), and 90-150 mg/L colchicine, inducing explant cells, and simultaneously doubling chromosomes and differentiating the adventitious buds. The present disclosure fills the blank of using a root of B. balsamifera as the explant and increases effective explant sources during the propagation, proliferation and biotechnological breeding of B. balsamifera. More importantly, root cells of the B. balsamifera are directly differentiated into adventitious buds while chromosomes are doubled, and a callus formation process is not needed, so that the technical links are simplified and the variation of regeneration buds and the generation of chimeras are reduced.

The present disclosure provides a method for obtaining adventitious tetraploid buds of Blumea balsamifera, comprising the following steps: selecting a root segment of diploid B. balsamifera as an explant, culturing the root segment in a chromosome doubling inducing medium supplemented with 0.025-0.1 mg/L 1-naphthaleneacetic acid (NAA), 1.0-2.0 mg/L 6-benzylaminopurine (6-BA), and 90-150 mg/L colchicine, inducing explant cells, and simultaneously doubling chromosomes and differentiating the adventitious buds. The present disclosure fills the blank of using a root of B. balsamifera as the explant and increases effective explant sources during the propagation, proliferation and biotechnological breeding of B. balsamifera. More importantly, root cells of the B. balsamifera are directly differentiated into adventitious buds while chromosomes are doubled, and a callus formation process is not needed, so that the technical links are simplified and the variation of regeneration buds and the generation of chimeras are reduced.

A wheat variety designated W060347A1, the plants and seeds of wheat variety W060347A1, methods for producing a wheat plant produced by crossing the variety W060347A1 with another wheat plant, and hybrid wheat seeds and plants produced by crossing the variety W060347A1 with another wheat line or plant, and the creation of variants by backcrossing, mutagenesis or transformation of variety W060347A1 are disclosed. Methods for producing other wheat varieties or breeding lines derived from wheat variety W060347A1 and to wheat varieties or breeding lines produced by those methods are also provided.

The present invention relates to the provision of technical means such as nucleic acids which, after transcription or after expression in a plant, are suitable for mediating the property of a haploid inductor or for increasing the induction capability of a haploid inductor, as well as methods and uses for the production and identification of non-transgenic and transgenic plant haploid inductors, as well as the improvement of existing plant haploid inductors.

Methods and compositions for improved plant breeding using gene editing and haploid induction are provided.

Methods and compositions for improved plant breeding using gene editing and haploid induction are provided.