Methods for preserving viability of plant tissues such as plant embryos are provided herein. Also included are methods for storing genomic DNA and/or molecular marker assay materials in an oil bilayer as part of a high-throughput molecular characterization system. Moreover, plant embryos may be treated while in an oil matrix. The treatment may include chromosome doubling, Agrobacterium-mediated transformation, or herbicide selection as part of an embryo rescue process.

A novel maize variety designated X90F453 and seed, plants and plant parts thereof are produced by crossing inbred maize varieties. Methods for producing a maize plant by crossing hybrid maize variety X90F453 with another maize plant are disclosed. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into X90F453 through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. This invention relates to the maize variety X90F453, the seed, the plant produced from the seed, and variants, mutants, and minor modifications of maize variety X90F453. This invention further relates to methods for producing maize varieties derived from maize variety X90F453.

A novel maize variety designated X85F778 and seed, plants and plant parts thereof are produced by crossing inbred maize varieties. Methods for producing a maize plant by crossing hybrid maize variety X85F778 with another maize plant are disclosed. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into X85F778 through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. This invention relates to the maize variety X85F778, the seed, the plant produced from the seed, and variants, mutants, and minor modifications of maize variety X85F778. This invention further relates to methods for producing maize varieties derived from maize variety X85F778.

According to the invention, there is provided seed and plants of the hybrid corn variety designated CH479340. The invention thus relates to the plants, seeds and tissue cultures of the variety CH479340, and to methods for producing a corn plant produced by crossing a corn plant of variety CH479340 with itself or with another corn plant, such as a plant of another variety. The invention further relates to genetic complements of plants of variety CH479340.

A novel maize variety designated X05F814 and seed, plants and plant parts thereof are produced by crossing inbred maize varieties. Methods for producing a maize plant by crossing hybrid maize variety X05F814 with another maize plant are disclosed. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into X05F814 through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. This invention relates to the maize variety X05F814, the seed, the plant produced from the seed, and variants, mutants, and minor modifications of maize variety X05F814. This invention further relates to methods for producing maize varieties derived from maize variety X05F814.

A novel maize variety designated X85F776 and seed, plants and plant parts thereof are produced by crossing inbred maize varieties. Methods for producing a maize plant by crossing hybrid maize variety X85F776 with another maize plant are disclosed. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into X85F776 through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. This invention relates to the maize variety X85F776, the seed, the plant produced from the seed, and variants, mutants, and minor modifications of maize variety X85F776. This invention further relates to methods for producing maize varieties derived from maize variety X85F776.

A novel maize variety designated X05F819 and seed, plants and plant parts thereof are produced by crossing inbred maize varieties. Methods for producing a maize plant by crossing hybrid maize variety X05F819 with another maize plant are disclosed. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into X05F819 through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. This invention relates to the maize variety X05F819, the seed, the plant produced from the seed, and variants, mutants, and minor modifications of maize variety X05F819. This invention further relates to methods for producing maize varieties derived from maize variety X05F819.

A novel maize variety designated X95H706 and seed, plants and plant parts thereof are produced by crossing inbred maize varieties. Methods for producing a maize plant by crossing hybrid maize variety X95H706 with another maize plant are disclosed. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into X95H706 through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. This invention relates to the maize variety X95H706, the seed, the plant produced from the seed, and variants, mutants, and minor modifications of maize variety X95H706. This invention further relates to methods for producing maize varieties derived from maize variety X95H706.

A switch to haploid embryogenesis is controlled by the activity of histone deacetylases (HDACs). Blocking HDAC activity with HDAC inhibitors (HDACi), e.g., trichostatin A (TSA), in Brassica napus, B. rapa, Arabidopsis thaliana, and Capsicum annuum male gametophytes leads to a large increase in the proportion of cells that undergo embryogenic growth. In B. napus, treatment with one specific HDACi (SAHA) improves the conversion (i.e., germination) of these embryos into seedlings. Existing methods of culturing microspores of angiosperm plants following stress to produce haploid embryos, haploid plants, and double haploid plants can be improved by adding HDACi to the culture medium. Advantageously, species hitherto recalcitrant to haploid embryogenesis via microspore culture are rendered useful when using HDACi. Haploid and double haploid plants are of industrial application in the plant breeding programmes.

A switch to haploid embryogenesis is controlled by the activity of histone deacetylases (HDACs). Blocking HDAC activity with HDAC inhibitors (HDACi), e.g., trichostatin A (TSA), in Brassica napus, B. rapa, Arabidopsis thaliana, and Capsicum annuum male gametophytes leads to a large increase in the proportion of cells that undergo embryogenic growth. In B. napus, treatment with one specific HDACi (SAHA) improves the conversion (i.e., germination) of these embryos into seedlings. Existing methods of culturing microspores of angiosperm plants following stress to produce haploid embryos, haploid plants, and double haploid plants can be improved by adding HDACi to the culture medium. Advantageously, species hitherto recalcitrant to haploid embryogenesis via microspore culture are rendered useful when using HDACi. Haploid and double haploid plants are of industrial application in the plant breeding programmes.