The present invention is directed to an edible composition including a plant belonging to the genus Solanum or plant part derived therefrom, wherein the plant or plant part is essentially devoid of steroidal glycoalkaloids (SGAs), and wherein the in vitro dry matter digestibility (IVDMD) of the plant or plant part is greater than or essentially equivalent to the IVDMD of a control plant belonging to the genus Solanum or plant part comprising SGAs. Further provided are a genetically modified plant belonging to the genus Solanum characterized by being essentially devoid of SGAs and having IVDMD greater than or essentially equivalent to the IVDMD of a control plant, and a method for producing same.

The present invention relates to tobacco products from plants comprising mutated berberine bridge enzyme-like nucleic acids and the recessive nic1 and/or nic2 alleles and methods of making the same.

Provided is a method for producing a low reversion type low nornicotine content tobacco plant line without use of a genetic modification technique or a factitious mutant preparation technique. Also provided is a tobacco plant. In the method in accordance with this disclosure, a variety-specific DNA sequence that links with a low reversion type low nornicotine character is determined, and selection is carried out with use of the sequence as an index.

The present invention relates to tobacco products from plants comprising mutated berberine bridge enzyme-like nucleic acids and the recessive nic1 and/or nic2 alleles and methods of making the same.

The present disclosure describes tobacco varieties having reduced levels of DVTs, methods of making such varieties, and tobacco compositions including such varieties.

There is described herein a plant cell having modulated expression or activity of a nicotinamidase enzyme, said plant cell comprising: (a) a polynucleotide sequence comprising, consisting or consisting essentially of a sequence encoding the nicotinamidase enzyme; or (b) a polypeptide encoded by the polynucleotide set forth in (a); wherein said plant cell comprises at least one modification which modulates the expression or activity of the polynucleotide or the polypeptide as compared to a control plant cell in which the expression or activity of the polynucleotide or polypeptide has not been modified. Furthermore, said poly nucleotide (a) of said plant cell comprises: (i) a polynucleotide comprising, consisting or consisting essentially of a sequence having at least 70% sequence identity to SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:15, SEQ ID NO:17, SEQ ID NO:19 SEQ ID NO:24 or SEQ ID NO:26; and/or (ii) a construct, vector or expression vector comprising the isolated polynucleotide set forth in (i) or set forth in SEQ ID NO:21. Furthermore, wherein said polypeptide (b) of said plant cell comprises: a polypeptide encoded by the polynucleotide set forth above; and/or a polypeptide comprising, consisting or consisting essentially of a sequence having at least 90% sequence identity to SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:25 or SEQ ID NO:27.

The present disclosure provides compositions and methods related to tobacco plants. In particular, the present disclosure provides novel methods for producing tobacco plants, and any related tobacco products, having low nicotinic alkaloid content. Tobacco plants produced according to the methods of the present disclosure exhibit reduced levels of nicotinic alkaloids (e.g., nicotine) compared to both naturally-occurring and transgenic tobacco plants, and thus represent a commercially valuable alternative to currently available tobacco varieties.

The present disclosure relates to compositions and methods related to tissue-specific promoters and their uses in plants, including tobacco and cannabis. The provided trichome-specific promoters enable the expression of heterologous polynucleotides in trichome tissues.

The invention discloses a tobacco NtbHLH13 gene mutant as well as a molecular identification method and application thereof. The tobacco NtbHLH13 gene mutant is Ntbhlhl3, and G at the 215th site of a tobacco Ntbhlhl3 gene is mutated into A to form a termination codon, so that the gene is terminated in advance, and the nucleotide sequence of the tobacco NtbHLH13 gene mutant is as shown in SEQ ID No. 1. After the gene of the tobacco NtbHLH13 gene mutant Ntbhlhl3 is mutated, the nicotine content of tobacco can be obviously increased, the tobacco quality is remarkably improved, and the gene mutanthas great value in tobacco breeding.

The present invention provides a method for modulating the alkaloid content of a plant (e.g. a tobacco plant), the method comprising modifying said plant by modulating the activity or expression of a homeodomain-like, Myb domain protein. The present invention also provides for the use of a homeodomain-like, Myb domain protein for modulating the alkaloid content of a plant, as well as tobacco cells, plants, plant propagation materials, harvested leaves, processed tobaccos, or tobacco products obtainable in accordance with the invention.