The present invention relates to optimized systems for the genome editing of eukaryotic cells, preferably plant cells. Also provided is a plant delivery system comprising at least one Cpf1 enzyme, or a plant optimized construct encoding the same, to be synergistically combined with a Cpf1 guide RNA system. Further provided is a Cpf1 guide RNA system being flanked by a Hammerhead ribozyme sequence at the 5 end and by a plant-derived Hepatitis Delta Vims (HDV)-like ribozyme sequence at the 3 end, or being embedded within a coding or non-coding region, of a sequence encoding a frame sequence. Novel plant-derived HDV-like ribozyme sequences are also provided. Further provided are methods for improved genome editing, and the use of the various systems provided herein to obtain transformed plants, plant cells, tissues, organs, or a progeny thereof, or a plant material, modified in a targeted way even at difficult to access target sites.

The present invention relates to a hybrid Swiss chard seed designated 69-600 RZ. The present invention also relates to a Swiss chard plant produced by growing the 69-600 RZ seed, which plant exhibits a combination of traits including having green leaves with white petioles and leaf midribs, resistance to downy mildew (Peronospora farinosa f. sp. betae), field resistance to Cercospora leaf spot, strong blistering of the leaf blade and a dark to very dark green leaf coloring. The invention further relates to methods for producing the Swiss chard cultivar, represented by hybrid Swiss chard variety 69-600 RZ.

The present invention relates to a hybrid Swiss chard seed designated 69-104 RZ. The present invention also relates to a Swiss chard plant produced by growing the 69-104 RZ seed, which plant exhibits a combination of traits including green leaves with a reddish purple petiole and midrib, slow growing, resistance to downy mildew (Peronospora farinosa f sp. betae), a dark to very dark green leaf coloring and strongly blistered leaf blades. The invention further relates to methods for producing the Swiss chard cultivar, represented by hybrid Swiss chard variety 69-104 RZ.

Provided are improved herbicide tolerant Beta vulgaris, particularly improved sugar beet plants, with increased yield performance, while maintaining optimal and agronomically relevant herbicide tolerance, wherein the large and small subunits of the herbicide tolerance acetolactate synthase enzyme are optimally fitted. Further provided are markers for identifying such improved herbicide tolerant Beta vulgaris plants, as well methods for obtaining and identifying such improved herbicide tolerant Beta vulgaris plants.

The present invention relates to the field of plant breeding and in particular to the regeneration of plants from cells and other tissues. More particularly, the invention provides methods and means for improving callus and shoot formation and regeneration of plants using hyperphyllin or derivatives thereof.

The present invention relates to optimized systems for the genome editing of eukaryotic cells, preferably plant cells. Also provided is a plant delivery system comprising at least one Cpf1 enzyme, or a plant optimized construct encoding the same, to be synergistically combined with a Cpf1 guide RNA system. Further provided is a Cpf1 guide RNA system being flanked by a Hammerhead ribozyme sequence at the 5 end and by a plant-derived Hepatitis Delta Virus (HDV)-like ribozyme sequence at the 3 end, or being embedded within a coding or non-coding region, of a sequence encoding a frame sequence. Novel plant-derived HDV-like ribozyme sequences are also provided. Further provided are methods for improved genome editing, and the use of the various systems provided herein to obtain transformed plants, plant cells, tissues, organs, or a progeny thereof, or a plant material, modified in a targeted way even at difficult to access target sites.

The present invention relates to a method for the production of haploid, polyhaploid and/or doubled haploid embryos, calli, seeds and/or plants of the species Beta vulgaris from isolated microspore cultures, more specifically to a method comprising contacting the isolated microspores with a histone deacetylase inhibitor (HDACi) and a complex protein composition. The present invention also provides a kit for producing a haploid, polyhaploid and/or doubled haploid embryo, callus, seed and/or plant of the species Beta vulgaris from at least one isolated microspore as well as the use of a histone deacetylase inhibitor (HDACi) and a complex protein composition for producing a haploid, polyhaploid and/or doubled haploid embryo, callus, seed and/or plant of the species Beta vulgaris. Finally, the present invention also relates to a population of haploid, polyhaploid and/or doubled haploid Beta vulgaris plants directly derived from a single flower, single inflorescence or single bud.

The present invention relates to a hybrid Swiss chard seed designated 69-600 RZ. The present invention also relates to a Swiss chard plant produced by growing the 69-600 RZ seed, which plant exhibits a combination of traits including having green leaves with white petioles and leaf midribs, resistance to downy mildew (Peronospora farinosa f. sp. betae), field resistance to Cercospora leaf spot, strong blistering of the leaf blade and a dark to very dark green leaf coloring. The invention further relates to methods for producing the Swiss chard cultivar, represented by hybrid Swiss chard variety 69-600 RZ.

A more efficient breeding against Cercospora leaf spot disease, or the development of new resistant lines, is enabled via the provision of the Cercospora resistance-mediating gene according to the invention; in particular, a dominant resistance effect in the target plant is evoked by the property of the identified gene alone. The Cercospora resistance-mediating gene, and embodiments of the present invention that are described in the preceding, offer additional applications, e.g., the use of the resistant gene allele in cis-genetic or trans-genetic approaches, with the goal of developing new resistant cultivars.

Provided are improved herbicide tolerant Beta vulgaris hybrid plants and parts thereof, particularly improved hybrid sugar beet or fodder beet plants, with increased yield performance, while maintaining optimal and agronomically relevant herbicide tolerance, wherein both parent plants are derived by introgression of the herbicide tolerance gene from a single herbicide resistance donor genotype. Further provided are Beta vulgaris parent plants useful for obtaining such hybrid plants, markers for identifying such improved herbicide tolerant Beta vulgaris plants, as well methods for obtaining and identifying such improved herbicide tolerant Beta vulgaris hybrid plants.