The invention provides a new and distinct hybrid variety of Spinach, NUN 06303 SPS as well as seeds and plants and leaves thereof.

The disclosure provides a new and distinct hybrid variety of spinach, NUN 05048 SPS as well as seeds and plants and fruits thereof.

The present invention relates to a method for modifying the resistance profile of a spinach plant to Peronospora farinosa f sp. spinaciae, comprising introducing a WOLF allele or a resistance-conferring part thereof into the genome of said spinach plant, or modifying an endogenous WOLF allele in the genome of said spinach plant. A WOLF allele encodes a CC-NBS-LRR protein that comprises in its amino acid sequence: the motif MAEIGYSVC at its N-terminus, and the motif KWMCLR for alpha-type WOLF proteins or HVGCVVDR for beta-type WOLF proteins. The invention relates to the WOLF alleles referred to in Table 3. The invention further provides a method for selecting a spinach plant comprising a novel WOLF gene that confers resistance to Peronospora farinosa f. sp. spinaciae in a spinach plant and a method for identifying a WOLF allele that confers resistance to one or more pathogenic races of Peronospora farinosa f sp. spinaciae in a spinach plant and to primers for use in these methods.

The present invention relates to an allele designated alpha-WOLF 15 which confers resistance to at least one Peronospora farinosa f. Sp. spinacea race, wherein the protein encoded by said allele is a CC-NBS-LRR protein that comprises in its amino acid sequence: a) the motif MAEIGYSVC at its N-terminus; and b) the motif KWMCLR; and wherein the LRR domain of the protein has in order of increased preference at least 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100% sequence similarity to SEQ ID No:10. The allele when homozygously present in a spinach plant confers complete resistance to Peronospora farinosa f. Sp. spinacea races pfs:1, pfs:2, pfs:3, pfs:4 and pfs:5, pfs:6, pfs:8, pfs:9, pfs:11, pfs:12, pfs:13, pfs:14, pfs:15, pfs:16 and isolates UA1014 and US1508, and confers intermediate resistance to pfs:10, and does not confer resistance to pfs:7.

The invention provides a new and distinct hybrid variety of Spinach, NUN 06258 SPS as well as seeds and plants and leaves thereof.

The present invention relates to an allele designated alpha-CMV which confers resistance to CMV when present in a spinach plant, wherein the protein encoded by said allele is a CC-NBS-LRR protein that comprises in its amino acid sequence: a) the motif MAEIGYSVC at its N-terminus; and b) the motif KWMCLR; and c) an LRR domain that has in order of increased preference at least 95%, 96%, 97%, 98%, 99%, 100% sequence similarity to SEQ ID No:10. The allele has a genomic nucleotide sequence which in order of increased preference has at least 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100% sequence similarity to SEQ ID No:1. The invention further relates to plants comprising the allele and to various methods involving the allele.

The invention provides a new and distinct hybrid variety of Spinach, NUN 05004 SPS as well as seeds and plants and leaves thereof.

The present invention relates to an allele designated alpha-WOLF 15 which confers resistance to at least one Peronospora farinosa f Sp. spinacea race, wherein the protein encoded by said allele is a CC-NBS-LRR protein that comprises in its amino acid sequence: a) the motif MAEIGYSVC at its N-terminus; and b) the motif KWMCLR; and wherein the LRR domain of the protein has in order of increased preference at least 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100% sequence similarity to SEQ ID No:10. The allele when homozygously present in a spinach plant confers complete resistance to Peronospora farinosa f. Sp. spinacea races pfs:1, pfs:2, pfs:3, pfs:4 and pfs: 5, pfs:6, pfs:8, pfs:9, pfs:11, pfs:12, pfs:13, pfs:14, pfs:15, pfs:16 and isolates UA1014 and US1508, and confers intermediate resistance to pfs:10, and does not confer resistance to pfs:7.

The present invention relates to the field of spinach breeding, in particular to a new dominant resistance gene, designated RPF12, which confers resistance against all races of Peronospora farinosa and to spinach plants comprising said gene.

The invention provides seed and plants of spinach hybrid SVVB5191 and the parent lines thereof. The invention thus relates to the plants, seeds and tissue cultures of spinach hybrid SVVB5191 and the parent lines thereof, and to methods for producing a spinach plant produced by crossing such plants with themselves or with another spinach plant, such as a plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of such plants, including the leaf and gametes of such plants.