Materials and methods for creating canola (e.g., Brassica napus) lines having oil with increased oleic acid content are provided herein.

The present invention relates to methods and compositions for transforming soybean, corn, cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.

The present invention relates to excision of explant material comprising meristematic tissue from seeds, and storage of such material prior to subsequent use in plant tissue culture and genetic transformation. Methods for tissue preparation, storage, and transformation are disclosed, as is transformable meristem tissue produced by such methods, and apparati for tissue preparation.

Provided is a canola variety designated 16GG0344L and seed, plants and plant parts thereof produced from a cross of inbred varieties. Methods for producing a canola variety comprise crossing canola variety 16GG0344L with another canola plant. Methods for producing a canola plant containing in its genetic material one or more traits introgressed into 16GG0344L through backcross conversion and/or transformation, and to the canola seed, plant and plant part produced thereby are described. Canola variety 16GG0344L, the seed, the plant produced from the seed, plant parts and variants, mutants, and minor modifications of canola variety 16GG0344L are disclosed.

Provided is a canola variety designated 16GG0337L and seed, plants and plant parts thereof produced from a cross of inbred varieties. Methods for producing a canola variety comprise crossing canola variety 16GG0337L with another canola plant. Methods for producing a canola plant containing in its genetic material one or more traits introgressed into 16GG0337L through backcross conversion and/or transformation, and to the canola seed, plant and plant part produced thereby are described. Canola variety 16GG0337L, the seed, the plant produced from the seed, plant parts and variants, mutants, and minor modifications of canola variety 16GG0337L are disclosed.

Provided is a canola variety designated 4006801-18 and seed, plants and plant parts thereof produced from a cross of inbred varieties. Methods for producing a canola variety comprise crossing canola variety 4006801-18 with another canola plant. Methods for producing a canola plant containing in its genetic material one or more traits introgressed into 4006801-18 through backcross conversion and/or transformation, and to the canola seed, plant and plant part produced thereby are described. Canola variety 4006801-18, the seed, the plant produced from the seed, plant parts and variants, mutants, and minor modifications of canola variety 4006801-18 are disclosed.

A novel canola variety designated G00555 and seed, plants and plant parts thereof. Methods for producing a canola plant that comprise crossing canola variety G00555 with another canola plant. Methods for producing a canola plant containing in its genetic material one or more traits introgressed into G00555 through backcross conversion and/or transformation, and to the canola seed, plant and plant part produced thereby. Hybrid canola seed, plant or plant part produced by crossing the canola variety G00555 or a locus conversion of G00555 with another canola variety.

A novel canola variety designated G5264590R and seed, plants and plant parts thereof. Methods for producing a canola plant that comprise crossing canola variety G5264590R with another canola plant. Methods for producing a canola plant containing in its genetic material one or more traits introgressed into G5264590R through backcross conversion and/or transformation, and to the canola seed, plant and plant part produced thereby. Hybrid canola seed, plant or plant part produced by crossing the canola variety G5264590R or a locus conversion of G5264590R with another canola variety.

Provided are various aspects related to increasing the proportion of omega-3 fatty acid in seed oil produced by a plurality of Brassica plants, which have been modified to produce seed oil comprising at least one of EPA, DHA and DPA. It relates to a method to increase the proportion of long-chain omega-3 fatty acid in seed oil produced by a plurality of Brassica oilseed plants that have been modified to produce seed oil with at least one of EPA, DHA and DPA comprising harvesting said Brassica plants when 80% to 100% color change has occurred on the first raceme.

A novel canola variety designated G00182 and seed, plants and plant parts thereof. Methods for producing a canola plant that comprise crossing canola variety G00182 with another canola plant. Methods for producing a canola plant containing in its genetic material one or more traits introgressed into G00182 through backcross conversion and/or transformation, and to the canola seed, plant and plant part produced thereby. Canola seed, plant or plant part produced by crossing the canola variety G00182 or a locus conversion of G00182 with another canola variety.