The present invention relates to novel watermelon plants displaying an increased number of male flowers. The present invention also relates to seeds and parts of said plants, for example fruits. The present invention further relates to methods of making and using such seeds and plants. The present invention also relates to novel genetic determinants associated with an increased number of male flowers and to molecular markers linked to said novel genetic determinants.

The disclosure provides a new and distinct watermelon variety NUN 31501 WMW as well as seeds and plants and fruits thereof. NUN 31501 WMW is a mini triploid, seedless watermelon variety, comprising resistance to Colletotrichum orbiculare Race 1.

The disclosure provides a new and distinct watermelon variety NUN 31607 WMW as well as seeds and plants and fruits thereof. NUN 31607 WMW is a triploid, mini crimson sweet type, seedless watermelon variety, comprising resistance to Colletorichum orbiculare Race 1.

Disclosed are systems and methods for whole-genome replacement through a massively multiplexed homologous template repair process. Disclosed aspects include a method of substantially changing a DNA sequence of an organism, the method including one or more of the following steps: determining a desired DNA sequence, the desired DNA sequence being a DNA sequence to which it is desired that the DNA sequence of the organism be substantially changed; preparing a treatment configured to cause the organism DNA sequence to be substantially changed to the desired DNA sequence; applying the treatment to the organism; wherein the treatment is configured to cause the organism DNA sequence to be substantially changed to the desired DNA sequence by causing, at each of multiple sites in the organism DNA sequence, genetic code at the site to be substantially changed to genetic code at a responding site in the desired DNA sequence; each of the multiple sites in the organism DNA sequence is a respective sub-sequence of the organism DNA sequence; applying the treatment includes delivering to the organism at least one dose; and each dose includes respective change agent material that causes the changing of the genetic code at a respective plurality of the multiple sites. In certain embodiments, the DNA sequence of the organism is a whole-genome DNA sequence of the organism, and the desired DNA sequence is substantially the organism's germline whole-genome DNA sequence, an intentionally modified version of the organism's germline whole-genome DNA sequence, or a whole-genome DNA sequence of another organism.

The disclosure provides a new and distinct watermelon variety NUN 91501 WMR as well as seeds and plants and rootstock thereof. NUN 91501 WMR is a watermelon variety comprising resistance to Fusarium oxysporum f s.p niveum Race 0, Race 1, and Race 2, and Verticillium dahliae.

Provided are an electron transport chain module from a eukaryotic organelle and an application thereof in biological nitrogen fixation. The electron transport chain (ETC) module is composed of the NifJ protein from Klebsiella oxytoca and a ferredoxin from plant chloroplasts or leucoplasts; plant-type ferredoxin-NADPH reductase (FNR) and the FdxH or FdxB protein from Anabaena; or an FNR and a Ferredoxin protein from plant organelles.

A new and distinct watermelon variety NUN 11802 WMW is disclosed as well as seeds and plants and fruits thereof. NUN 11802 is a round oval, diploid watermelon variety of the mini-seeded type, comprising resistance to Fusarium oxysporum f. s.p niveum Race 0 and Race 1.

The present invention is directed to watermelon plant and seed, namely of C. lanatus, which are resistant to geminiviruses, especially to begomoviruses including WmCSV, comprising in their genome introgressed sequences from C. colocynthis conferring resistance to said viruses, when present homozygously. The introgressed sequences are preferably characterized by defined alleles of SNPs on chromosome 6. The introgressed sequences can be chosen from those present in the genome of a plant of Citrullus lanatusWMCSVR accession number NCIMB 42197. The invention is also directed to parts of these resistant plants, as well as progeny, to the use of these plants for introgressing the resistance in another genetic background, as well as to different methods for obtaining resistant watermelon plants or seeds.

The present invention relates to the dual, independent cistron expression system in a single vector for the production of protein of interest proteins and peptides expressed as insoluble inclusion bodies formed in the bacteria E. coli. The present invention also provides the process for the expression of protein of interest using said bicistronic vector.

The disclosure provides a new and distinct watermelon variety NUN 31707 WMW as well as seeds and plants and fruits thereof. NUN 31707 WMW is a triploid, crimson sweet type watermelon variety, comprises resistance to Colletorichum orbiculare Race 1 and Fusarium oxysporum f. s.p niveum Race 1. The fruit of watermelon variety NUN 31707 WMW is round large and is seedless.