The present invention relates to the dual, independent cistron expression system in a single vector for the production of protein of interest proteins and peptides expressed as insoluble inclusion bodies formed in the bacteria E. coli. The present invention also provides the process for the expression of protein of interest using said bicistronic vector.

The application relates to the field of plant breeding, in particular watermelon breeding. Provided are bush type, triploid watermelon plants (and seeds from which these plants can be grown) and seedless watermelon fruits produced by these plants. Also provided are bush type pollenizer plants and bush type tetraploid plants and methods for producing triploid hybrids having a bush growth type, as well as methods for producing seedless watermelon fruits of high quality.

The present disclosure provides, among other things, watermelon characterized by traits useful for production of seedless watermelon fruit and/or watermelon fruit with a commercially desirable spotted phenotype. The present disclosure provides, among other things, QTLs and SNPs useful for production of watermelon characterized by traits for production of seedless fruit and/or fruit having a spotted phenotype.

The present invention is a novel method which results in the production of small seedless watermelon fruit having an average fruit weight of less than 12.0 pounds. The novel method involves using small tetraploid parental lines to produce small triploid hybrid seed.

The present invention provides novel watermelon line 54WA004 and plant parts, seed, and tissue culture therefrom. The invention also provides methods for producing a watermelon plant by crossing the watermelon plants of the invention with themselves or another watermelon plant. The invention also provides watermelon plants produced from such a crossing as well as plant parts, seed, and tissue culture therefrom. Further provided are methods of producing triploid watermelon seed and plants and seedless watermelon fruit produced therefrom as well as the triploid watermelon seed and plants and the seedless fruits produced by such methods.

The invention provides seed and plants of watermelon line TCS-EJ09134. The invention thus relates to the plants, seeds, and tissue cultures of watermelon line TCS-EJ09134 and to methods for producing a watermelon plant produced by crossing such plants with themselves or with another watermelon plant, such as a plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to plants, seeds, plant parts, and tissue cultures of watermelon line TCS-EJ09134 comprising introduced beneficial or desirable traits.

The present invention relates to a modified gene that provides resistance to Cucurbit Yellow Stunting Disorder virus (CYSDV) when present in a plant of the Cucurbitaceae family. The invention further relates to progeny, seed, and fruit of the plant that is resistant against CYSDV. The invention also relates to propagation material suitable for producing the plant that is resistant to CYSDV. Additionally, the invention relates to methods for producing, identifying, and selecting a plant of the Cucurbitaceae family having resistance against CYSDV.

A new watermelon variety designated E26C00199 is described. E26C00199 is a mini watermelon variety with high yields.

The invention relates to the field of Citrullus lanatus, in particular to a new variety of watermelon designated NUN 7201 WMW as well as plants, seeds and watermelon fruits thereof.

The present invention relates to methods of producing unique varieties of triploid watermelon with diverse rind patterning. More specifically, a new watermelon variety designated Harvest Moon is described. Harvest Moon is a seedless, spotted watermelon variety with improved flesh quality and deeper red color than traditional spotted watermelon varieties.