A novel maize variety designated X85P966 and seed, plants and plant parts thereof are produced by crossing inbred maize varieties. Methods for producing a maize plant by crossing hybrid maize variety X85P966 with another maize plant are disclosed. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into X85P966 through backcrossing or genetic transformation, and to the maize seed, plant and plant part produced thereby are described. Maize variety X85P966, the seed, the plant produced from the seed, and variants, mutants, and minor modifications of maize variety X85P966 are provided. Methods for producing maize varieties derived from maize variety X85P966 and methods of using maize variety X85P966 are disclosed.

A novel maize variety designated PH4CYJ and seed, plants and plant parts thereof are provided. Methods for producing a maize plant comprise crossing maize variety PH4CYJ with another maize plant are provided. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PH4CYJ through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby are provided. Hybrid maize seed, plants or plant parts are produced by crossing the variety PH4CYJ or a locus conversion of PH4CYJ with another maize variety.

The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms.

Compositions and methods are provided for introducing transgenic target sites for Site Specific Integration (SSI) and/or polynucleotides of interest into at least one double-strand break target site of a double-strand-break inducing agent in a genomic window of a plant genome. Also provided are methods and compositions for producing a complex trait locus in a genomic window of a plant comprising at least one transgenic target site for site specific integration integrated in at least double-strand-break target site. The double-strand-break target site can be, but is not limited to, a target site for a zinc finger endonuclease, an engineered endonuclease, a meganuclease, a TALENs and/or a Cas endonuclease. The genomic window of said plant can comprise at least one genomic locus of interest such as a trait cassette, a transgene, a mutated gene, a native gene, an edited gene or a site-specific integration (SSI) target site.

This invention relates to compositions and methods for modifying CLAVATA3/EMBRYO SURROUNDING REGION-RELATED (CLV3/ESR-RELATED) (CLE) genes in plants to increase increased kernel row number, optionally without substantially decreasing the length of the ears. The invention further relates to plants having increased kernel row number, optionally without substantially decreasing the length of the ears, produced using the methods and compositions of the invention.

The present invention relates to a method for introducing a substance into a plant. The method of the present invention comprises the steps of: obtaining an enzymatically treated and isolated fertilized egg cell by (1-i) isolating a fertilized egg cell from a plant tissue containing a fertilized egg cell, and then treating the fertilized egg cell with an enzyme solution containing a plant tissue-degrading enzyme under a low-titer condition, (1-ii) treating a plant tissue containing a fertilized egg cell with an enzyme solution containing a plant tissue-degrading enzyme under a low-titer condition, and then isolating the fertilized egg cell that has been enzymatically treated, (1-iii) treating a plant tissue containing a fertilized egg cell with an enzyme solution containing a plant tissue-degrading enzyme under a low-titer condition, and simultaneously isolating the fertilized egg cell that has been enzymatically treated, (1-iv) isolating an egg cell and a sperm cell from a plant to produce a fertilized egg by fusing the cells, and then treating the fertilized egg cell with an enzyme solution containing a plant tissue-degrading enzyme under a low-titer condition, or (1-v) treating a plant tissue containing an egg cell with an enzyme solution containing a plant tissue-degrading enzyme under a low-titer condition, and then isolating the egg cell that has been enzymatically treated, and further fusing the egg cell with an isolated sperm cell; and (2) introducing a substance selected from the group consisting of nucleic acids, proteins, and peptides into the resultant enzymatically treated and isolated fertilized egg cell.

Embodiments disclosed herein relate to the field of plant molecular biology, specifically to DNA constructs for conferring insect resistance to a plant. Embodiments disclosed herein relate to insect resistant corn plant containing event DP-915635-4, and to assays for detecting the presence of event DP-915635-4 in samples and compositions thereof

Compositions and methods for generating maize plants that exhibit resistance to northern leaf blight are provided herein. Polynucleotides encoding a polypeptide that confers resistance to northern leaf blight, polynucleotide constructs comprising such, and maize plants comprising the polynucleotide constructs are provided.

A novel maize variety designated 1PACB31 and seed, plants and plant parts thereof are provided. Methods for producing a maize plant comprise crossing maize variety 1PACB31 with another maize plant are provided. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into 1PACB31 through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby are provided. Hybrid maize seed, plants or plant parts are produced by crossing the variety 1PACB31 or a locus conversion of 1PACB31 with another maize variety.

A novel maize variety designated PH48PP and seed, plants and plant parts thereof are provided. Methods for producing a maize plant comprise crossing maize variety PH48PP with another maize plant are provided. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PH48PP through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby are provided. Hybrid maize seed, plants or plant parts are produced by crossing the variety PH48PP or a locus conversion of PH48PP with another maize variety.