Embodiments of the present disclosure are directed to systems and methods for collection and analysis of environmental air dust (EAD) within an individually ventilated cage rack (IVR) environment for detecting pathogens. The method includes collection of an EAD sample by a collection media, isolation of a plurality of nucleic acids (e.g., RNA and/or DNA) representative of one or more infectious agents from the EAD sample, optional reverse transcription of RNA to cDNA if the isolated nucleic acids contain RNA, amplification of the cDNA and/or DNA (e.g., by polymerase chain reaction (PCR)), and assay interpretation. Optionally, the EAD sample may be analyzed with one or more other sample types (e.g., fecal pellets, oral swabs, body swabs, tissue, etc.) to improve detection of low-copy organisms.

Embodiments of the present disclosure are directed to systems and methods for collection and analysis of environmental air dust (EAD) within an individually ventilated cage rack (IVR) environment for detecting pathogens. The method includes collection of an EAD sample by a collection media, isolation of a plurality of nucleic acids (e.g., RNA and/or DNA) representative of one or more infectious agents from the EAD sample, optional reverse transcription of RNA to cDNA if the isolated nucleic acids contain RNA, amplification of the cDNA and/or DNA (e.g., by polymerase chain reaction (PCR)), and assay interpretation. Optionally, the EAD sample may be analyzed with one or more other sample types (e.g., fecal pellets, oral swabs, body swabs, tissue, etc.) to improve detection of low-copy organisms.

A pet enclosure includes a bottom section, a middle section, and a top section. The middle section is connectable to the bottom section with an opening in the middle section. The opening in the middle section has a bottom portion that connects with a top portion of an opening in the bottom section. The top section is connectable to the middle section with an opening in the top section. The opening in the top section has a bottom portion that connects with a top portion of an opening in the bottom section. The bottom section, middle section and top section when connected together form an enclosed pet enclosure with an animal opening therein.

A disposable animal cage which can be used in a ventilated or static caging rack system includes a cage top and a cage base. Any corner or edge present in the interior of the cage base or the cage top that is accessible to an animal that can be contained in the cage is defined by surfaces having a conical section which is non-circular to prevent gnawing of the cage. The cage top can include a water housing mount pocket. The water housing mount pocket can have a conical shape to prevent an animal from gnawing the water housing mount pocket and a container received in the water housing mount pocket. A feed tray can be attached beneath a filter in a ceiling of the cage top to prevent an animal from gnawing the filter.

Embodiments of the present disclosure are directed to systems and methods for collection and analysis of environmental air dust (EAD) within an individually ventilated cage rack (IVR) environment for detecting pathogens. The method includes collection of an EAD sample by a collection media, isolation of a plurality of nucleic acids (e.g., RNA and/or DNA) representative of one or more infectious agents from the EAD sample, optional reverse transcription of RNA to cDNA if the isolated nucleic acids contain RNA, amplification of the cDNA and/or DNA (e.g., by polymerase chain reaction (PCR)), and assay interpretation. Optionally, the EAD sample may be analyzed with one or more other sample types (e.g., fecal pellets, oral swabs, body swabs, tissue, etc.) to improve detection of low-copy organisms.

The present disclosure provides compositions and methods for long-term storage, shipping, and stable production of black soldier flies, capable of use in waste remediation or protein production. The invention overcomes drawbacks associated with current methods of using black soldier flies, significantly increasing the period during which black soldier fly neonates can be stored and the time in which larvae mature.

The present invention relates to a ventilation system for pig-raising facilities, comprising: a pig house (110) comprising a plurality of pig rooms (a) in which pigs are raised, and comprising an intermediate ceiling (113) and an upper ceiling (115) which are spaced apart from each other; a pit (120) provided at the bottom of the plurality of pig rooms (a); a plurality of roof exhaust fans (140) arranged at positions corresponding to the plurality of pig rooms (a), along the edge regions of the intermediate ceiling (113); and a plurality of air entry slots (150) provided in a plate surface of the intermediate ceiling (113) at constant intervals. At the front surface of the pig house (110), there are provided a lower window (114) which is provided between the intermediate ceiling (113) and the upper ceiling (115) and through which external air is introduced, and an upper window (116) which is provided between the upper ceiling (115) and a roof (112) and through which external air is introduced. At the rear surface of the pig house (110), lower exhaust fans (117) are provided to face the lower window (114), and upper exhaust fans (118) are provided to face the upper window (116).

A pet carrier including a bottom portion and a top portion having an attachment portion secured to the back wall of the bottom portion and extending away from the back wall of the bottom portion distal the front wall of the bottom portion, wherein the attachment portion of the bottom portion rotatably secures to the attachment portion of the top portion, a first lobe extends from a portion of the first side wall distal the ceiling, and a second lob extends from a portion of the second side wall distal the ceiling.

A door assembly includes a frame having interconnected wires that define an opening for an animal to pass therethrough, and a door having interconnected wires and being coupled to the frame. A latch assembly has a handle structure and a pin. The handle structure is pivotable with respect to one of the door and the frame, and the pin is movable axially with respect to one of the door and the frame. A first wire of the plurality of wires of the frame forms a key hole assembly. The key hole assembly defines an aperture for receiving the pin when the door is in a latched position with respect to the frame. A tab assembly is formed by at least one wire of the door and is disposable behind at least one of the plurality of wires of the frame in the closed position.

The invention relates to a climate control device for controlling the temperature and humidity of air supplied to colonies of insects cultured in an industrial scale insect farm. In particular, the invention relates to a controllable air conditioning system involving a network of pipes connecting at least one cluster having at least one insect farming cage, such that air is conditionable in the cages at the cluster level. More specifically, the invention relates to a system having a central main air conditioning facility providing temperature conditioned air and absolute air humidity conditioned air to each of a number of local air conditioning devices, each separate local air conditioning device providing temperature conditioned air and absolute air humidity conditioned air to each of a plethora of clusters of insect breeding cages separately, said cages housed in a temperature conditioned farming room. This way, insects in cages housed in farming rooms are farmed under improved conditions with regard to minimized cage-to-cage temperature differences and differences in relative air humidity, due to the use of the climate control device of the invention.