In some examples, a package includes a package body enclosing a medical supply. The package body defines a window. The package includes a breathable membrane adjacent the package body and extending across the window. The package includes a moisture oxygen scavenger adjacent the package body and extending across the breathable membrane. The package includes an adhesive layer configured to hold the moisture oxygen scavenger adjacent the breathable membrane. In some examples, a technique includes sterilizing a package precursor including the package body and the breathable membrane, and after the sterilizing, adhering the moisture oxygen scavenger to the package body to form the package.

In some examples, a package includes a package body enclosing a medical supply. The package body defines a window. The package includes a breathable membrane adjacent the package body and extending across the window. The package includes a moisture oxygen scavenger adjacent the package body and extending across the breathable membrane. The package includes an adhesive layer configured to hold the moisture oxygen scavenger adjacent the breathable membrane. In some examples, a technique includes sterilizing a package precursor including the package body and the breathable membrane, and after the sterilizing, adhering the moisture oxygen scavenger to the package body to form the package.

A method for manufacturing a packaging with an intraocular lens enclosed therein includes insertion of the intraocular lens into a packaging, comprising a first packaging material which is gas-tight in a first sub-section, and a second packaging material which is permeable to gases in a second sub-section, first sealing of the packaging so that the intraocular lens is enclosed in the interior space of the packaging, the interior space being partially delimited by the first sub-section and the second sub-section, sterilization of the interior space of the packaging by exposing the packaging to a gas so that the gas can penetrate through the second sub-section into the interior space of the packaging, second sealing of the packaging by sealing off a volume of the gas-tight first sub-section of the packaging with the intraocular lens enclosed therein from the volume of the second sub-section.

There is described a packaging apparatus (1) for forming a plurality of sealed packages (2) from a tube (3) of a web of packaging material (4) which is continuously filled with a pourable product. The packaging apparatus comprises a delimiting element (37) arranged, in use, within the tube (3) and being designed to divide the tube (3), in use, into a first space (38) and a second space (39). The packaging apparatus (1) also comprises a sterile gas supply device (43) for pressurizing the isolation chamber and the second space (39) by means of a respectively a first flow of sterile gas and a second flow of sterile gas.

A method of sterilizing a prefilled syringe comprises assembling a syringe assembly and performing at least one ethylene oxide (EtO) sterilization procedure cycle. The assembling step includes inserting a tip cap at a first end of a plunger, filling a non-glass barrel with sterilization sensitive material at a second end of the barrel, and inserting the plunger into the barrel at a second end to seal the sterilization sensitive material within the barrel. The EtO sterilization procedure cycle comprises undergoing a preprocessing stage, a wash and conditioning stage, an EtO sterilization stage and a wash and post exposure stage. Upon completing the EtO sterilization procedure cycle, a resultant pH of the sterilization-sensitive material does not exceed an acceptable pH range as defined by the United States Pharmacopeia. The disclosure also discusses inserting an assembled prefilled syringe into a procedure tray prior to performing the EtO sterilization procedure cycle.

A method of sterilizing a prefilled syringe comprises assembling a syringe assembly and performing at least one ethylene oxide (EtO) sterilization procedure cycle. The assembling step includes inserting a tip cap at a first end of a plunger, filling a non-glass barrel with sterilization sensitive material at a second end of the barrel, and inserting the plunger into the barrel at a second end to seal the sterilization sensitive material within the barrel. The EtO sterilization procedure cycle comprises undergoing a preprocessing stage, a wash and conditioning stage, an EtO sterilization stage and a wash and post exposure stage. Upon completing the EtO sterilization procedure cycle, a resultant pH of the sterilization-sensitive material does not exceed an acceptable pH range as defined by the United States Pharmacopeia. The disclosure also discusses inserting an assembled prefilled syringe into a procedure tray prior to performing the EtO sterilization procedure cycle.

Systems and methods for filling and sealing a container can include a housing having an opening for receiving containers, a rotary assembly including mount assemblies disposed in the housing, the rotary assembly operable to rotate the of mount assemblies about a central axis, a washing station, a filling station, a steam injector, and cap setter disposed in the housing.

A filling machine configured to form, fill, and seal individual packages is provided. The filling machine comprises a sterilization station configured to provide a flow of gaseous sterilizing agent towards open ends of ready-to-fill packaging containers passing through said sterilization station. The filling machine comprises at least one clean air supply and at least one baffle plate arranged above the at least one clean air supply, wherein the air supply is configured to direct a stream of clean air towards the ready-to-fill packaging containers. The clean air supply in turn comprises at least one distribution pipe and wherein the distribution pipe extends in a horizontal direction, the horizontal direction being in the direction of the flow of packages through the filling machine.

A filling machine configured to form, fill, and seal individual packages is provided. The filling machine comprises a sterilization station configured to provide a flow of gaseous sterilizing agent towards open ends of ready-to-fill packaging containers passing through said sterilization station. The filling machine comprises at least one clean air supply and at least one baffle plate arranged above the at least one clean air supply, wherein the air supply is configured to direct a stream of clean air towards the ready-to-fill packaging containers. The clean air supply in turn comprises at least one distribution pipe and wherein the distribution pipe extends in a horizontal direction, the horizontal direction being in the direction of the flow of packages through the filling machine.

Methods, assays, compositions and kits for the ligation of short polynucleotides are presented herein. The short polynucleotides are optionally no more than 7 nucleotides in length, and can be as short as 3 or 4 nucleotides in length. The ligation is optionally performed by CV ligase.