Provided is a method developed for producing a thread bundle with high efficiency in terms of spun thread collection and without causing mechanical damage to a foothold silk thread spun by a bagworm onto a base material. After a bagworm is placed along with a solvent-soluble base material and allowed to spin the foothold silk thread onto the surface of the base material, the base material is dissolved with a solvent to separate the foothold silk thread therefrom.

Provided is a method developed for producing a thread bundle with high efficiency in terms of spun thread collection and without causing mechanical damage to a foothold silk thread spun by a bagworm onto a base material. After a bagworm is placed along with a solvent-soluble base material and allowed to spin the foothold silk thread onto the surface of the base material, the base material is dissolved with a solvent to separate the foothold silk thread therefrom.

It is intended to develop and provide a technique of conveniently allowing a transgenic silkworm by itself and at an individual level to produce a recombinant protein having a mammalian-type sugar chain sialic acid attached thereto, without the need of a baculovirus expression system or oral and transdermal administration of sialic acid. An expression vector was developed which can induce the expression of a mammalian-type glycosylation-related gene group only in a silk gland such that the recombinant protein modified with the mammalian-type sugar chain has no adverse effect on the silkworm itself. A transgenic silkworm harboring the expression vector was prepared.

The present disclosure provides a warm water immersion-based hatching method of silkworm eggs, belonging to the technical field of sericulture production. In the present disclosure, a warm water treatment is conducted on silkworm eggs of a bivoltine silkworm variety in production, and a water temperature and an immersion time are optimized to ensure a higher hatching rate. The silkworm eggs are subjected to the warm water immersion at 49? C. to 51? C. for 15 s to 23 s to rapidly hatch the silkworm eggs without diapause.

The present disclosure provides a warm water immersion-based hatching method of silkworm eggs, belonging to the technical field of sericulture production. In the present disclosure, a warm water treatment is conducted on silkworm eggs of a bivoltine silkworm variety in production, and a water temperature and an immersion time are optimized to ensure a higher hatching rate. The silkworm eggs are subjected to the warm water immersion at 49? C. to 51? C. for 15 s to 23 s to rapidly hatch the silkworm eggs without diapause.

For sorting silkworm chrysalises, a feed mechanism positions silkworm chrysalises on a delivery mechanism. The delivery mechanism receives and carries the silkworm chrysalises from the feed mechanism. A detection mechanism detects a female silkworm chrysalis based on an optical wavelength emitted by the female silkworm chrysalis. A sorting mechanism that sorts the silkworm chrysalises, putting female silkworm chrysalises into a female collecting box, and male silkworm chrysalises into male collecting box.

For sexing silkworm cocoons, a delivery mechanism carries a checker cocooning frame holding silkworm cocoons. A detection mechanism detects a female silkworm cocoon based on an optical wavelength emitted by the female silkworm cocoon. A sorting mechanism sorts the silkworm cocoons, putting female silkworm cocoons into a female collecting box, and male silkworm cocoons into a male collecting box

For sexing silkworm cocoons, a delivery mechanism carries a checker cocooning frame holding silkworm cocoons. A detection mechanism detects a female silkworm cocoon based on an optical wavelength emitted by the female silkworm cocoon. A sorting mechanism sorts the silkworm cocoons, putting female silkworm cocoons into a female collecting box, and male silkworm cocoons into a male collecting box

Transgenic silkworms stably expressing synthetic spider silk genes or composite silkworm/spider silk genes are disclosed. The exogenous spider silk genes are stably intergrated into a defined site of the fibroin heavy chain intron or a fibroin light chain intron of silkworms. Synthetic spider silk proteins and composite spider silk-silkworm genes and proteins are provided. The expression of exogenous spider silk genes is driven by the endogenous fibroin heavy chain promoter, improving the genetic stability of transgenic silkworms. The composite silkworm/spider silk fibers exhibit exceptional mechanical performance, compared to normal silkworm silk fibers and other transgenic silkworm fibers.

Transgenic silkworms stably expressing synthetic spider silk genes or composite silkworm/spider silk genes are disclosed. The exogenous spider silk genes are stably intergrated into a defined site of the fibroin heavy chain intron or a fibroin light chain intron of silkworms. Synthetic spider silk proteins and composite spider silk-silkworm genes and proteins are provided. The expression of exogenous spider silk genes is driven by the endogenous fibroin heavy chain promoter, improving the genetic stability of transgenic silkworms. The composite silkworm/spider silk fibers exhibit exceptional mechanical performance, compared to normal silkworm silk fibers and other transgenic silkworm fibers.