The present disclosure provides compositions with a modulating gene expression and methods for modulating transcription.

A system for batch production of the heterogametic sex of a biological species generally includes a first strain of a biological species genetically engineered to include a conditional Y-linked (or Z-linked) genetic lethal circuit and a second strain of the biological species genetically engineered to include a conditional X-linked (or W-linked) genetic lethal circuit.

The first object of the invention is a production line for rearing and/or breeding insects and/or larval forms of insects of the order Coleoptera and/or Diptera, characterized in that it comprises: at least one breeding line (14) for breeding insects for laying feed thereon and a flow-through feed heating and/or cooling system (1) with a closed flow of heating-cooling medium for heating/cooling the feed on the breeding line (14). The second object is a method for breeding insects including a step of rearing and/or breeding insects and/or larval forms of insects using a production line according to the invention. A further object is a method for breeding insects using a flow-through feed heating and/or cooling system during the breeding. Another object is the use of a flow-through feed heating and/or cooling system (1) with a closed flow of heating- cooling medium for heating/cooling the feed on the breeding line (14).

One variation of a method includes: during an initial period, modifying a population of insects to produce a target compound responsive to application of a stressor; during a growth period succeeding the initial period, cultivating the population of insects according to a set of growth conditions; and, during a treatment period succeeding the growth period, applying a dosage of the stressor to the population of insects to trigger production of the target compound. The method further includes, during a harvest period succeeding the treatment period: harvesting the population of insects; homogenizing the population of insects to form a blend including a set of secondary components and an amount of the target compound; extracting the amount of the target compound from the blend; and mixing the first amount of the first target compound with a set of stabilizing agents configured to stabilize the target compound.

A device comprising a dispenser assembly comprising a frame having a first surface and a second surface, wherein the frame defines a pathway to enable movement of a moveable member within the frame, an inlet opening in the first surface of the frame and a drain opening in the second surface of the frame, the inlet opening and the drain opening providing access to the pathway, wherein the frame further comprises a filter overlaying the drain opening; and a moveable member translatable within the pathway between a first position and a second position, the moveable member defining at least one bore, wherein at least one of the at least one bore is aligned with the inlet opening at the first position; and a reservoir container coupled to the dispenser assembly, the reservoir container defining a dispensing opening, the dispensing opening aligned with the inlet opening of the frame.

The first object of the invention is a production line for rearing and/or breeding insects and/or larval forms of insects of the order Coleoptera and/or Diptera, characterized in that it comprises: at least one breeding line (14) for breeding insects for laying feed thereon and a flow-through feed heating and/or cooling system (1) with a closed flow of heating-cooling medium for heating/cooling the feed on the breeding line (14). The second object is a method for breeding insects including a step of rearing and/or breeding insects and/or larval forms of insects using a production line according to the invention. A further object is a method for breeding insects using a flow-through feed heating and/or cooling system during the breeding. Another object is the use of a flow-through feed heating and/or cooling system (1) with a closed flow of heating-cooling medium for heating/cooling the feed on the breeding line (14).

Problem to be Solved: The present invention is intended to provide a polynucleotide encoding the light-chain variable region and the heavy-chain variable region of an anti-dog IgE antibody; and an anti-dog IgE antibody containing these variable regions. Solution: The present invention is DNA encoding a heavy-chain variable region consisting of the amino acid sequence represented by SEQ ID NO: 2 or 6 and DNA encoding a light-chain variable region consisting of the amino acid sequence represented by SEQ ID NO: 4 or 8, and an anti-dog IgE monoclonal antibody which binds to dog IgE, containing these variable regions or a functional fragment thereof which binds to dog IgE.

Provided is a gene expression system, suitable for expression in an insect, comprising an insect muscle actin promoter operably linked to a marker gene, which overcomes or ameliorates one or more of: cost of rearing; amount of handling; errors in identification due to human error or loss of marker by the insect; and health concerns related to the effects of marker powders on workers in mass rearing facilities.

The present invention pertains to a silkworm-type biotinylated CTLD14 or sRAGE and a method for manufacturing the same. One embodiment of the present invention provides a method for manufacturing biotinylated proteins, wherein the method includes A) a step for inserting a nucleic acid molecule for coding biotin ligase and protein in a coexpressable manner into a silkworm or a living organism that imparts sugar chains that are the same as the sugar chains of the silkworm, B) a step for causing the biotin ligase and protein to be expressed by disposing the silkworm or the living organism that imparts sugar chains that are the same as the sugar chains of the silkworm to conditions with which the nucleic acid molecule will carry out expression, and C) a step for administering biotin to the living organism and obtaining the biotinylated protein.

The present invention provides compositions and methods for light-activated cation channel proteins and their uses within cell membranes and subcellular regions. The invention provides for proteins, nucleic acids, vectors and methods for genetically targeted expression of light-activated cation channels to specific cells or defined cell populations. In particular the invention provides millisecond-timescale temporal control of cation channels using moderate light intensities in cells, cell lines, transgenic animals, and humans. The invention provides for optically generating electrical spikes in nerve cells and other excitable cells useful for driving neuronal networks, drug screening, and therapy.