The first object of the invention is a production line for rearing and/or breeding insects and/or larval forms of insects of the order Coleoptera and/or Diptera, characterized in that it comprises: at least one breeding line (14) for breeding insects for laying feed thereon and a flow-through feed heating and/or cooling system (1) with a closed flow of heating-cooling medium for heating/cooling the feed on the breeding line (14). The second object is a method for breeding insects including a step of rearing and/or breeding insects and/or larval forms of insects using a production line according to the invention. A further object is a method for breeding insects using a flow-through feed heating and/or cooling system during the breeding. Another object is the use of a flow-through feed heating and/or cooling system (1) with a closed flow of heating- cooling medium for heating/cooling the feed on the breeding line (14).

The first object of the invention is a production line for rearing and/or breeding insects and/or larval forms of insects of the order Coleoptera and/or Diptera, characterized in that it comprises: at least one breeding line (14) for breeding insects for laying feed thereon and a flow-through feed heating and/or cooling system (1) with a closed flow of heating-cooling medium for heating/cooling the feed on the breeding line (14). The second object is a method for breeding insects including a step of rearing and/or breeding insects and/or larval forms of insects using a production line according to the invention. A further object is a method for breeding insects using a flow-through feed heating and/or cooling system during the breeding. Another object is the use of a flow-through feed heating and/or cooling system (1) with a closed flow of heating- cooling medium for heating/cooling the feed on the breeding line (14).

A sieving device is described. The sieving device includes an adjustable sieve surface that includes a first sieve surface and a second sieve surface. Openings are formed in each of the first sieve surface and the second sieve surface so as to define a shared pathways extending through the adjustable sieve surface. The shared pathways are defined by a length dimension that is greater than a width dimension. The width dimension can correspond to a cephalothorax width of an insect.

A sieving device is described. The sieving device includes an adjustable sieve surface that includes a first sieve surface and a second sieve surface. Openings are formed in each of the first sieve surface and the second sieve surface so as to define a shared pathways extending through the adjustable sieve surface. The shared pathways are defined by a length dimension that is greater than a width dimension. The width dimension can correspond to a cephalothorax width of an insect.

A male population of mosquito pupae sorted according to a sorting method is described. The sorting method may include adding a mixed population of mosquito pupae into an interior volume of a sieving container including base defining a set of openings enabling movement of mosquito pupae from the interior volume through the set of openings. The mixed population of mosquito pupae may include male mosquito pupae and female mosquito pupae. The method may also include adding a volume of aqueous solution to the interior volume. The method may also include removing a portion of the male pupae from the mixed population of mosquito pupae to by performing a sieving action that causes the portion of the male pupae and a portion of the volume of water to pass from the interior volume through the set of openings. The method may also include capturing the portion of the male pupae as the male population of mosquito pupae after the portion of the male pupae has passed through the set of openings.

A male population of mosquito pupae sorted according to a sorting method is described. The sorting method may include adding a mixed population of mosquito pupae into an interior volume of a sieving container including base defining a set of openings enabling movement of mosquito pupae from the interior volume through the set of openings. The mixed population of mosquito pupae may include male mosquito pupae and female mosquito pupae. The method may also include adding a volume of aqueous solution to the interior volume. The method may also include removing a portion of the male pupae from the mixed population of mosquito pupae to by performing a sieving action that causes the portion of the male pupae and a portion of the volume of water to pass from the interior volume through the set of openings. The method may also include capturing the portion of the male pupae as the male population of mosquito pupae after the portion of the male pupae has passed through the set of openings.

The present invention relates to insect-based biowaste processing apparatus. More specifically, the present invention relates to a substantially continuous insect-based biowaste processor that comprises a tubular drum having a longitudinal axis and an interior; a drive for rotatably driving said drum about said axis; a shaftless screw conveyor fixedly connected to an inner surface of said drum; a plurality of circumferentially spaced cantilevered blades that are connected to said inner drum surface and that each longitudinally extend throughout said drum; and means for introducing a conglomerate portion that includes biowaste and insect larvae into said drum interior, wherein said screw conveyor is subdivided into a plurality of longitudinally spaced rearing chambers for the introduced insect larvae, each of said rearing chambers being defined by two longitudinally adjacent flights of said screw conveyor and by said circumferentially spaced cantilevered blades and within which insect larvae of a substantially uniform developmental stage are retained, wherein two or more of said circumferentially spaced cantilevered blades are configured to firmly hold and unify said conglomerate portion at any given instance throughout its residing time within said drum interior while being distally conveyed, and wherein the insect larvae are progressively more developed within a more distally located rearing chamber.

The present invention relates to insect-based biowaste processing apparatus. More specifically, the present invention relates to a substantially continuous insect-based biowaste processor that comprises a tubular drum having a longitudinal axis and an interior; a drive for rotatably driving said drum about said axis; a shaftless screw conveyor fixedly connected to an inner surface of said drum; a plurality of circumferentially spaced cantilevered blades that are connected to said inner drum surface and that each longitudinally extend throughout said drum; and means for introducing a conglomerate portion that includes biowaste and insect larvae into said drum interior, wherein said screw conveyor is subdivided into a plurality of longitudinally spaced rearing chambers for the introduced insect larvae, each of said rearing chambers being defined by two longitudinally adjacent flights of said screw conveyor and by said circumferentially spaced cantilevered blades and within which insect larvae of a substantially uniform developmental stage are retained, wherein two or more of said circumferentially spaced cantilevered blades are configured to firmly hold and unify said conglomerate portion at any given instance throughout its residing time within said drum interior while being distally conveyed, and wherein the insect larvae are progressively more developed within a more distally located rearing chamber.

One variation of a method includes: during an initial period, modifying a population of insects to produce a target compound responsive to application of a stressor; during a growth period succeeding the initial period, cultivating the population of insects according to a set of growth conditions; and, during a treatment period succeeding the growth period, applying a dosage of the stressor to the population of insects to trigger production of the target compound. The method further includes, during a harvest period succeeding the treatment period: harvesting the population of insects; homogenizing the population of insects to form a blend including a set of secondary components and an amount of the target compound; extracting the amount of the target compound from the blend; and mixing the first amount of the first target compound with a set of stabilizing agents configured to stabilize the target compound.

The present disclosure provides transgenic non-human animal (e.g., nematode) systems for assessing heterologous polygenic or monogenic phenotypes, their variants and drug discovery. The transgenic non-human animals (e.g., nematodes) contain a first heterologous polypeptide coding sequence and a second heterologous polypeptide coding sequence (a plurality of heterologous polypeptide coding sequences), wherein the first and second heterologous polypeptide coding sequences are integrated into the host animal genome, and wherein expression of the first and second heterologous polypeptide coding sequence contribute to the heterologous phenotype. The plurality of heterologous polypeptide coding sequences are interrelated wherein their expression products, directly or indirectly, contribute or lead to an observable phenotype.