A new on chip system, named Acoustic Gym, to apply a surface acoustic wave (SAW) has been developed to control the swimming exercise of a large number of small animals such as C. elegans and Zebra fish, at desired intensities and durations. The SAW device consists of a circular chamber filled with fluid, which is placed in the center between a pair of offset interdigital transducers (IDTs) that are deposited on a piezoelectric substrate. Upon actuation from a radio frequency (RF) source, the SAW is generated and propagates along the substrate towards the microchamber. Once the SAW reaches the chamber, it induces an acoustic streaming in the fluid inside the chamber to stimulate swimming exercise of small animals. The streaming velocity can be precisely controlled via the RF signal input power, thus regulating the duration and intensity of exercise of the animals.

A new on chip system, named Acoustic Gym, to apply a surface acoustic wave (SAW) has been developed to control the swimming exercise of a large number of small animals such as C. elegans and Zebra fish, at desired intensities and durations. The SAW device consists of a circular chamber filled with fluid, which is placed in the center between a pair of offset interdigital transducers (IDTs) that are deposited on a piezoelectric substrate. Upon actuation from a radio frequency (RF) source, the SAW is generated and propagates along the substrate towards the microchamber. Once the SAW reaches the chamber, it induces an acoustic streaming in the fluid inside the chamber to stimulate swimming exercise of small animals. The streaming velocity can be precisely controlled via the RF signal input power, thus regulating the duration and intensity of exercise of the animals.

The present invention relates to a method for screening an anticancer agent by causing drosophila having the characteristics of a) expression of mutant Ras85D, b) deletion or suppressed expression of a p53 gene, c) overexpression of a cyclin E gene, and d) deletion or suppressed expression of a Med gene to ingest a test substance and comparing the survival rate thereof with the survival rate of drosophila that did not ingest the test substance. The present invention also relates to a combination drug of at least two kinase inhibitors for treatment of pancreatic cancer and to kinase inhibitors for use in said combination drug.

The present invention relates to a method for screening an anticancer agent by causing drosophila having the characteristics of a) expression of mutant Ras85D, b) deletion or suppressed expression of a p53 gene, c) overexpression of a cyclin E gene, and d) deletion or suppressed expression of a Med gene to ingest a test substance and comparing the survival rate thereof with the survival rate of drosophila that did not ingest the test substance. The present invention also relates to a combination drug of at least two kinase inhibitors for treatment of pancreatic cancer and to kinase inhibitors for use in said combination drug.

A sieving apparatus for separating insect pupa is described. In an example, the sieving apparatus includes a sieving device attached to an actuation system. The sieving device includes a set of openings sized to correspond to a cross-sectional cephalothorax width of a representative pupa of a population of insect pupae to be separated. The actuation system is configured to move the sieving device in a manner that causes smaller insect pupae to pass through the set of openings. Larger insect pupae remain within the sieving device. Such movement can include moving the sieving device with respect to a liquid held in a basin of the sieving apparatus.

A sieving apparatus for separating insect pupa is described. In an example, the sieving apparatus includes a sieving device attached to an actuation system. The sieving device includes a set of openings sized to correspond to a cross-sectional cephalothorax width of a representative pupa of a population of insect pupae to be separated. The actuation system is configured to move the sieving device in a manner that causes smaller insect pupae to pass through the set of openings. Larger insect pupae remain within the sieving device. Such movement can include moving the sieving device with respect to a liquid held in a basin of the sieving apparatus.

Apparatus (1000) and method for automatically detecting odorant substances based on use of nematodes that includes a mechanical selection unit (100) configured to select nematodes in adult stage from an initial nematode population obtaining an intermediate nematode population, a nematode optical selection unit (200) configured to select from the intermediate population a final population of nematodes in adult stage and to select nematodes in young adult stage from nematodes in egg producing adult stage to be sent to a measurement unit (300) configured to detect the response of nematodes of the final population to a stimulus of an odorant substance, the mechanical selection unit (100) being connected to the optical selection unit (200) by a connection channel with an at least three way branch and the optical selection unit (200) being connected to the measurement unit (300) by a loading microchannel.

Breeding comb for solitary bees is comprised of nesting sheets stacked one on top of another and containing grooves forming nesting tunnels (1) of varying sizes, lugs (2) placed over the side edges of the sheet and on the back wall, locks in the form of lugs (4) in the lower part of the base of the nesting sheet forming pass-through channels (5), slide guides (6) placed in the front part of the sheet, interfaces (8) placed on outer sides of the sheets where the individual nesting sheets are separated from each other by a membrane (3), with a shutter (7) placed at the front part of the breeding comb containing inlet holes (9) of varying sizes corresponding to the sizes of the nesting tunnels (1), and holes (10) corresponding to the sizes of the pass-through channels (5).

Devices, systems, and methods for transporting or transferring insect pupae in an aqueous solution are described. The system includes a tank containing a solution with insect pupae, a transfer chamber having an outlet at a bottom portion of the chamber, a solution inlet, an evacuation channel, and a depth measurement system. A tube fluidly connects to tank and the transfer chamber. An evacuation device connected to the evacuation channel removes air from the transfer chamber and generates a vacuum therein to draw the solution through the tube into the transfer chamber.

Devices, systems, and methods for transporting or transferring insect pupae in an aqueous solution are described. The system includes a tank containing a solution with insect pupae, a transfer chamber having an outlet at a bottom portion of the chamber, a solution inlet, an evacuation channel, and a depth measurement system. A tube fluidly connects to tank and the transfer chamber. An evacuation device connected to the evacuation channel removes air from the transfer chamber and generates a vacuum therein to draw the solution through the tube into the transfer chamber.