The invention is an apparatus for providing fresh and sanitary drinking water or other delivery fluids to insects. Embodiments of the invention provide this fluid on an automated and continual basis during the breeding and growing of insects. Embodiments of the invention provide a continuous supply of fresh water to insects during the entirety of the growing cycle. The current system allows for an automated supply of water to be fed into the system on a continual basis. The current system also provides for the continuous cleaning and sanitation of the system to insure continued sanitary operation and suitability of the fluid.

The invention is an apparatus for providing fresh and sanitary drinking water or other delivery fluids to insects. Embodiments of the invention provide this fluid on an automated and continual basis during the breeding and growing of insects. Embodiments of the invention provide a continuous supply of fresh water to insects during the entirety of the growing cycle. The current system allows for an automated supply of water to be fed into the system on a continual basis. The current system also provides for the continuous cleaning and sanitation of the system to insure continued sanitary operation and suitability of the fluid.

An array system of passive solar aquaponic and mushroom production structures, each having a glazing on a sun facing side to maximize winter sunlight; a plant growing area; a mushroom growing area; a water wall thermal mass integrated and disposed between the plant and mushroom growing areas; a fish tank; a saltwater basin under the plant growing holding saltwater; a natural air ventilation system providing misted air to the mushroom growing area and receiving O2 from the plant growing provided to the mushroom growing area, and CO2 generated by the mushroom growing area provided to the plant growing area; and saltwater channels delivering saltwater to the saltwater basins from a saltwater source, and which is evaporated by solar heat generated by the plant growing areas in order to generate freshwater.

An array system of passive solar aquaponic and mushroom production structures, each having a glazing on a sun facing side to maximize winter sunlight; a plant growing area; a mushroom growing area; a water wall thermal mass integrated and disposed between the plant and mushroom growing areas; a fish tank; a saltwater basin under the plant growing holding saltwater; a natural air ventilation system providing misted air to the mushroom growing area and receiving O2 from the plant growing provided to the mushroom growing area, and CO2 generated by the mushroom growing area provided to the plant growing area; and saltwater channels delivering saltwater to the saltwater basins from a saltwater source, and which is evaporated by solar heat generated by the plant growing areas in order to generate freshwater.

A fragile substance storage transportation and release device comprises a frame for inserting cartridges to hold the fragile substance; a propulsion unit for propelling the fragile substance out of successive cartridges, cartridge by cartridge; and an opening mechanism for opening each cartridge one by one in coordination with a propulsion mechanism. The device is useful for distribution of fragile insects such as mosquitoes and there is a mechanism for automatic collection of insects from pupae.

A fragile substance storage transportation and release device comprises a frame for inserting cartridges to hold the fragile substance; a propulsion unit for propelling the fragile substance out of successive cartridges, cartridge by cartridge; and an opening mechanism for opening each cartridge one by one in coordination with a propulsion mechanism. The device is useful for distribution of fragile insects such as mosquitoes and there is a mechanism for automatic collection of insects from pupae.

The disclosure provides a method of generating a sterile fish, crustacean, or mollusk. The method comprises breeding (i) a fertile hemizygous mutated female fish, crustacean, or mollusk with (ii) a fertile hemizygous mutated male fish, crustacean, or mollusk, selecting a female progenitor that is homozygous by genotypic selection, and breeding the homozygous female progenitor to produce the sterile fish, crustacean, or mollusk. The mutation disrupts the maternal-effect of a primordial germ cell (PGC) development gene and does not impair the viability, sex determination, fertility, or a combination thereof, of a homozygous progenitor. The disclosure also provides methods of making broodstock freshwater and seawater organisms for use in producing sterilized freshwater and seawater organisms, as well as the broodstock itself.

The first object of the invention is a production line for rearing and/or breeding insects and/or larval forms of insects of the order Coleoptera and/or Diptera, characterized in that it comprises: at least one breeding line (14) for breeding insects for laying feed thereon and a flow-through feed heating and/or cooling system (1) with a closed flow of heating-cooling medium for heating/cooling the feed on the breeding line (14). The second object is a method for breeding insects including a step of rearing and/or breeding insects and/or larval forms of insects using a production line according to the invention. A further object is a method for breeding insects using a flow-through feed heating and/or cooling system during the breeding. Another object is the use of a flow-through feed heating and/or cooling system (1) with a closed flow of heating-cooling medium for heating/cooling the feed on the breeding line (14).

The first object of the invention is a production line for rearing and/or breeding insects and/or larval forms of insects of the order Coleoptera and/or Diptera, characterized in that it comprises: at least one breeding line (14) for breeding insects for laying feed thereon and a flow-through feed heating and/or cooling system (1) with a closed flow of heating-cooling medium for heating/cooling the feed on the breeding line (14). The second object is a method for breeding insects including a step of rearing and/or breeding insects and/or larval forms of insects using a production line according to the invention. A further object is a method for breeding insects using a flow-through feed heating and/or cooling system during the breeding. Another object is the use of a flow-through feed heating and/or cooling system (1) with a closed flow of heating-cooling medium for heating/cooling the feed on the breeding line (14).

The present invention provides compositions and methods for inducing suspended animation in neonate larvae of beneficial fly, particularly larvae of the Black soldier fly. During the suspended animation, the larvae show extended shelf-life and improved shipment durability. The suspended animation is reversible, and upon exposure to standard rearing conditions the larvae reach comparable maturity to corresponding non-suspended larvae.