The invention relates to a method for cell defrosting, comprising a step of defrosting a cell suspension consisting of a freezing medium and of cells, followed by a step of removing said freezing medium by filtration, said step of removing the freezing medium being carried out without the previous or simultaneous supply of medium. In particular, the filter is hydrophobic or, before addition of the cell suspension to be frozen, has been covered with a hydrophobic liquid forming a temporary barrier between the filter and the cell suspension. During defrosting, said hydrophobic liquid is removed by the application of a driving force which makes the liquid pass through the filter.

The present invention relates to a gas delivery device comprising a gas releasing molecule and a gas permeable membrane. The invention also relates to the use of the gas delivery device for delivery of gas to an extracorporeal transplant, extracorporeal cells, a brain-dead transplant donor or foodstuff and in therapy. The invention further relates to the use of a gas permeable and liquid and solid impermeable membrane to separate a gas releasing molecule and its non-gaseous degradation products from an extracorporeal transplant, extracorporeal cells, a brain-dead transplant donor or foodstuff.

A storage device includes a plurality of storage locations on a carousel, as well as a tube picker adapted to access tubes in a tube rack located in a one of several nests by a tube gripper. The tube picker is arranged at the top of the carousel. The nests are rotatable with the carousel while the tube gripper is stationary. The same tube rack handler is provided for moving tube racks between the storage locations, the nests, and an external system.

A sample processing unit (SPU)-equipped drone for transporting and processing biological materials and method of using same is disclosed. In some embodiments, the presently disclosed SPU-equipped drone and method provide a drone equipped to carry an SPU and wherein the SPU may include a centrifuge arranged inside a temperature-controlled chamber and wherein the centrifuge may be used to process biological materials at the same time that the SPU-equipped drone is in flight. Further, a method of using the presently disclosed SPU-equipped drone for transporting and processing biological materials is provided.

The invention relates to a long term cell culturing method and a cell culturing apparatus and kit that employ a porous polyimide film. The invention further relates to a cell cryopreservation method and kit employing the porous polyimide film.

A system for the hypothermic transport of biological samples, such as tissues, organs, or body fluids. The system includes a self-purging preservation apparatus to suspend a sample in preservation fluid and perfuse a tissue with preservation fluid. The self-purging preservation apparatus is placed in an insulated transport container having a cooling medium. When assembled, the system allows for transport of biological samples for extended periods of time at a stable temperature.

Disclosed is portable organ transportation system including a mobile cart and an organ container. At least one of the mobile cart and the organ container include a latch for releasably attaching the organ container to the mobile cart. The organ container may be a portable organ preservation device or a portable organ perfusion apparatus.

A method for extracting stem cells from a non-embryonic stem cell source, including providing a non-embryonic stem cell source including stem cells; perfusing the non-embryonic stem cell source with a pulsatile flow of a perfusion solution to produce a perfusate including stem cells and a perfused non-embryonic stem cell source; and isolating the stem cells from the perfusate to produce isolated stem cells, is provided. Also provided is a non-embryonic stem cell line derived from a non-embryonic stem cell obtained using the pulsatile perfusion extraction method.

Methods and apparatus for preserving detached tissues, especially digits and limbs, which are detached as a result of traumatic amputation. By using the methods and apparatus, detached tissues can be preserved for greater lengths of time and are ultimately in a better condition for replantation surgery.

A specimen holder includes a body, a sleeve, and a wireless transponder. The body has distal and proximal portions, the distal portion including a surface that carries a specimen upon engagement of the body with the specimen, and the proximal portion including a first pair of parallel surfaces. The sleeve has distal and proximal portions, a side wall, and an internal cavity at least partially enclosed by the side wall. The distal portion of the sleeve includes a second pair of parallel surfaces. The wireless transponder is sized to be positioned within the internal cavity of the sleeve. The sleeve is attachable to the body by capturing one of the first and second pairs of parallel surfaces between the other of the first and second pairs of parallel surfaces.