This disclosure relates to a system and method to improve the uniformity of the heat transfer coefficient of containers filled with biological materials during the freezing and thawing process, in particular a system that is easily transported and incremented to other conventional freeze-thaw methods and equipment. The system includes a portable air blast system configured to receive a container filled with biological materials and to be placed in a cooling or heating chamber, for homogeneous and reproducible freezing and thawing of biological materials. This disclosure also relates to a method of freezing and thawing biological solution inside a container by using the portable air blast system according to any of the previous claims 1-12, comprising: obtaining a container filled with biological solution; placing the container into the vent enclosure and on the stand of the air blast system; placing the air blast system with the container into a heating or cooling chamber; activating the air blast system by activating the fan in the air blast system.

A packaging system is provided. The packaging system may include a packaging body defining a cavity. The packaging system may also include a tissue cradle configured to be disposed within the cavity. The packaging system may also include a seal configured to be joined to the packaging body to fluidly seal the cavity. Such packaging systems may be used in the storage of wet-preserved or dry-preserved human or animal tissue. Also provided are a packaged tissue specimen and a method for packaging tissue.

A method for damaged viable disc regeneration has the steps of identifying the damaged viable disc and inserting a cannula via Kambin's Triangle to an edge of an outer annulus of the disc; introducing a trocar into the cannula and penetrating the trocar into a central region of nucleus pulposus; removing a tissue biopsy sample from the nucleus pulposus for pathology and removing additional degenerative tissue from the central region to create a void or space; withdrawing the trocar from the cannula and inserting a needle into the cannula to the void or space; and injecting a regenerative disc material through the needle into the void or space to repair the damaged disc.

Disclosed are methods and compositions useful for treating patients with inflammatory, auto-immune and age related conditions. The disclosure relates to using Natural Killer cells and derivatives thereof in order to induce therapeutic benefit to patients affected by disease and conditions such as cancer, multiple sclerosis, rheumatoid arthritis, type I diabetes mellitus, thyroid autoimmune disease, psoriasis and inflammatory bowel diseases.

A method for cryopreserving a plurality of cell clusters (1, 2) of biological cells includes the steps of fractionating the cell clusters (1, 2) into at least two fractions (4) in dependency on at least one property of the cell clusters (1, 2), collecting the fractions (4) in different containers (21), and cryopreserving the cell clusters (1, 2) of the at least two fractions (4), wherein specific pretreatment methods and/or freezing methods are used for each fraction. A cryopreservation apparatus (100), for cryopreserving a plurality of cell clusters (1, 2) of biological cells, having a fractionation device, is also described.

An apparatus for cleaning transplant organs comprising an organ perfusion circuit and perfusion solution filtration circuit that removes virus and bacteria. The perfusion pump circuit has a cassette for containing perfusion fluid and an organ and a perfusion pump to circulate perfusion fluid in and out of the cassette. A perfusion solution filtering device has a filter and a pump coupled to the cassette to pump perfusion fluid out of the cassette, through the filter, and back into the cassette.

This disclosure provides a preserving composition comprising a cholesterol: carrier complex, optionally a cholesterol:cyclodextrin complex (CC complex) and/or a cell permeable antioxidant peptide and a biological buffer and optionally a cryprotectant, wherein the preserving composition is optionally substantially free of animal phospholipid, animal protein and/or animal lipoprotein. The disclosure also provides methods for the use of the preserving composition in cryopreservation of semen or sperm cells. Also provided is a kit comprising the preserving composition having a CC complex, a biological buffer, a cryoprotectant, a carbohydrate, a pH stabilizer, an antibiotic or antibiotic cocktail and/or a cell permeable anti-oxidant peptide.

A support system is disclosed to prepare harvested soft tissue for grafting. Methods of graft preparation are also disclosed.

A method of treating a harvested organ or tissue for preservation for implantation into a patient has the steps of, harvesting an organ or tissue from a donor; placing the harvested organ or tissue into a container; filling the container with a fluid for preservation; sealing the container once filled; directing one or more sound wave treatments into the container to destroy bacteria or molds or fungi or virus and to stimulate the organ or tissue; and storing the container at a hypothermic temperature of about 4 degrees C. for storage prior to implantation.

A kit, including: a first solution, a second solution, and a metal mesh. The first solution includes: dulbecco's modified eagle medium (DMEM), 65-95 V/V %; dimethyl sulfoxide (DMSO), 5.5-20 V/V %; ethylene glycol (EG), 3.5-15 V/V %; bovine serum albumin (BSA), 0.5-4 W/V %; sucrose 1-5 W/V %; methylcellulose with a viscosity of 4000 centipoise (cP), 0.05-0.8 W/V %; hetastarch 0.25-0.6 W/V %; and glucose 15-35 W/V %. The second solution includes: DMEM, 65-95 V/V %; DMSO, 5.5-20 V/V %; EG, 8-20 V/V %; BSA, 0.5-4 W/V %; sucrose, 10-20 W/V %; methylcellulose with a viscosity of 4000 centipoise (cP), 0.05-0.8 W/V %; polyvinyl pyrrolidone (PVP), 0.25-0.6 W/V %; and glucose 15-35 W/V %. The metal mesh has a thickness of 0.15-0.2 mm and includes a plurality of square holes. The side length of the square holes is 2.0-3.0 mm, and the spacing between adjacent holes is 0.5-2.0 mm.