In one embodiment, a fluid transfer component for transferring thermal energy comprises a film comprising a polymer with a thickness less than 5 millimeters, an input side constructed to receive fluid that flows from the input side to an active region of the film, more than 20 fluid channels defined by interior surfaces within the film, each fluid channel separated spatially in at least 1 row in a thickness direction of the film, the more than 20 fluid channels have a channel density across the active region greater than 5 fluid channels per centimeter, wherein the thermal energy is transferred to or from an environment and the fluid in the active region. The film may be an extruded microcapillary film or interior surfaces may comprise a surface modified to produce a surface relief profile. The active region may cool or warm the environment, which may comprise an individual.

The present disclosure relates to fluidic systems and devices for processing, extracting, or purifying one or more analytes. These systems and devices can be used for processing samples and extracting nucleic acids, for example by isotachophoresis. In particular, the systems and related methods can allow for extraction of nucleic acids, including non-crosslinked nucleic acids, from samples such as tissue or cells. The systems and devices can also be used for multiplex parallel sample processing.

A micromechanical sensor that is produced surface-micromechanically includes at least one mass element formed in a third functional layer that is non-perforated at least in certain portions. The sensor has a gap underneath the mass element that is formed by removal of a second functional layer and at least one oxide layer. The removal of the at least one oxide layer takes place by introducing a gaseous etching medium into a defined number of etching channels arranged substantially parallel to one another. The etching channels are configured to be connected to a vertical access channel in the third functional layer.

Techniques regarding microfluidic chips with one or more vias filled with sacrificial plugs and/or manufacturing methods thereof are provided herein. For example, one or more embodiments described herein can comprise an apparatus, which can comprise a silicon device layer of a microfluidic chip comprising a plurality of vias extending through the silicon device layer. The plurality of vias comprise greater than or equal to about 100 vias per square centimeter of a surface of the silicon device layer and less than or equal to about 100,000 vias per square centimeter of the surface of the silicon device layer. Additionally, the apparatus can comprise a plurality of sacrificial plugs positioned in the plurality of vias.

A liquid handling device has an accommodation part for accommodating a liquid, two or more flow paths each opening to a lower part of a side wall surface of the accommodation part, and a liquid movement suppression part that is disposed in the lower part of the side wall between the openings of two of the flow paths that are adjacent to each other and slows or stops the movement of the liquid along the corner formed by the lower surface of the accommodation part and the side wall surface.

Systems, methods, and devices for analyzing small volumes of fluidic samples, as a non-limiting example, less than twenty microliters are provided. The devices are configured to make a first sample reading, for example, measure an energy property of the fluid sample, for example, osmolality, make a second sample reading, for example, detecting the presence or concentration of one or more analytes in the fluid sample, or make both the first sample reading and the second sample reading, for example, measuring the energy property of the fluid sample as well as detecting the presence or concentration of one or more analytes in the fluid sample.

Methods for introducing exogenous material into a cell are provided, which include exposing the cell to a transient decrease in pressure in the presence of the exogenous material. Also provided are devices for performing the method of the invention.

A sample loading cartridge (1) for a microfluidic device comprises a cartridge body (10) with a sample reservoir (20) configured to house a volume of a liquid sample (3) and a sample port (30) in connection with the sample reservoir (20). The cartridge (1) also comprises an output channel (40) extending from the sample reservoir (20) and a feedback channel (50) connected to the sample reservoir (20) and to the sample port (30). The cartridge body (10) comprises a detection portion (60) aligned with the feedback channel (50) to enable detection of any sample (3) in the feedback channel (50). The flow resistance of the feedback channel (50) is lower than the flow resistance of the output channel (40) to cause liquid sample (3) received in the sample port (30) to enter the feedback channel (50) with substantially no liquid sample (3) entering the output channel (40).

A microfluidic apparatus has a microchannel that includes at least one vertically oriented segment with a top section having a relatively wide opening and a bottom section having a relatively narrow opening. The top section is larger in volume relative to the bottom sections, and the middle sections taper down in at least one dimension from the top section to the bottom section. One or tens or hundreds of vertically-oriented segments may be provided, and they are fluidly coupled to each other. Each segment acts as a pressure-volume-temperature (PVT) cell, and the microchannel apparatus may be used to determine a parameter of a fluid containing hydrocarbons such as the dew point of the fluid or the liquid drop-out as a function of pressure.

Provided is a method for producing a microchannel including an approximately circular cross section with neither a joined surface nor an inlet in a smaller number of steps than has been conventional. The method for producing a microchannel includes the steps of forming a layer of an uncured curable resin (2) on a substrate (1), inserting into the curable resin a needle body (3) that can inject a liquid (4), injecting a liquid in a tubular shape into the curable resin via the needle body while moving the needle body, extracting the needle body from the curable resin, and curing the curable resin to form a channel (4A) in a tubular region injected with the liquid.