A target component is collected using a preparative separation and purification device having a holder for holding a trap column in which the target component has been captured, a liquid feeder for feeding a first solvent having compatibility with the water remaining in the trap column and a second solvent having low compatibility with water and high compatibility with the first solvent into the trap column, a flow-path switch for connecting the exit end of the trap column to a waste liquid flow path and a collection flow path, and a control unit for controlling the flow-path switch so that solution including water flows into the waste liquid flow path.

A thermal impact assembly for a sample separation apparatus for separating a fluidic sample in a mobile phase by a sample separation unit includes a thermal impact device configured for thermally impacting the fluidic sample and/or the mobile phase and the sample separation unit, and a control unit configured for controlling the thermal impact device for thermally impacting the fluidic sample and/or the mobile phase on the one hand and for thermally impacting the sample separation unit on the other hand independently from each other.

Techniques are described for accelerating thermal equilibrium in a chromatographic column. An apparatus comprises a chromatography column, and a plurality of temperature control units in thermal contact with the chromatography column. A method of performing liquid chromatography comprises setting an inlet of a chromatography column to a first temperature using a first temperature control unit in thermal contact with said inlet, setting an outlet of the chromatography column to a second temperature using a second temperature control unit in thermal contact with the outlet, wherein the first temperature is less than the second temperature; and injecting a sample into a liquid stream that flows through the chromatography column after the inlet is set at the first temperature and the outlet is at the second temperature.

A method for making a caffeoylquinic composition from a botanical source is disclosed. The method may include chromatographing an extract of biomass on an ion exchange stationary phase and obtaining an eluent comprising a caffeoylquinic composition. The biomass may be Stevia or yerba mate, for example. The caffeoylquinic composition includes one or more of monocaffeoylquinic acid, dicaffeoylquinic acid, and salts of the foregoing.

Implemented is an analysis apparatus column oven equipped with a plurality of columns, which is capable of efficiently performing heat conduction on column basis, recognizing a column cartridge that holds a column, and being easily exchanged on column basis. The column change mechanism 105 includes the ferrule 206, the movable ferrule connector 207, the pipe 208, the fastener pull 209, the fastener fitting 210, the column cartridge presser 211, the slide guide 212, the RFID reader 214, the fixed wall 215, the column changer heat insulating member 216, the fixed bottom plate 217, the receiver 218, and the fixed fitting 219. A plurality of column cartridges 104 can be installed in the heat block 101, and are in contact with the column heat block 103 to control the temperature in the column cartridges 104. The column cartridge 104 is fixed between left and right column change mechanisms 105, and when the fixed column cartridge 104 is opened, the column change mechanism 105 on the left side is moved leftward and opened.

Thermally modulated variable restrictors used in chromatography systems enable independent control of system pressure and linear velocity of a compressible mobile phase passing through a chromatography column. A method for configuring a chromatography system with independent control of system pressure and mass flow rate of a compressible mobile phase includes determining a type of chromatography separation column to be used in the chromatography system, matching a thermally modulated variable restrictor to the type of chromatography separation column for use together during operation of the chromatography system, and bundling the chromatography column with its matching thermally modulated variable restrictor for distribution as a single package.

Apparatus and methods for performing chromatography may include a chromatography column and a vacuum insulated jacket having an inner wall and an outer wall. A vacuum area may be formed between the inner wall and the outer wall. The inner wall of the vacuum insulated jacket may surround the chromatography column. A gap may be formed between an outer wall of the chromatography column and the inner wall of the vacuum insulated jacket. The vacuum insulated jacket may extend beyond one or more end frits of the column. The gap may be filled with one or more materials so as to form an insulating or thermal barrier.

Provided is a method for producing a lithium-containing solution that prevents the dissolution of the whole lithium manganese oxide while maintaining the efficiency of an elution step. The method for producing a lithium-containing solution comprises performing an adsorption step of contacting a lithium adsorbent obtained from lithium manganese oxide with a low lithium-containing liquid for adsorption to give post-adsorption lithium manganese oxide, an elution step of contacting the post-adsorption lithium manganese oxide with an acid solution to give a lithium-containing solution with residual manganese, and a manganese oxidation step of oxidating manganese to give a lithium-containing solution with a suppressed manganese concentration, performed in this order. The acid solution is a 0.5 mol/L or more and 4.0 mol/L or less hydrochloric acid solution. According to the production method, in the elution step, the dissolution of the whole lithium manganese oxide can be suppressed while maintaining the efficiency of exchange reaction between cations including Li.sup.+ and H.sup.+. Thus, the repeated use of the lithium adsorbent becomes possible.

Solid phase extraction (SPE) sorbents and liquid chromatography (LC) stationary phases are provided, as well as methods of fabricating the same. The SPE sorbents and LC stationary phases can use microcrystalline cellulose particles as the substrate and sol-gel sorbent coating technology as the polymer/sorbent immobilization technology. The SPE sorbents and LC stationary phases are stable in a pH range of 1-13 and at a temperature of up to 350 ° C.

Provided is a supercritical fluid apparatus including: an analysis flow path through which a mobile phase flows; a back-pressure control valve provided at a downstream end of the analysis flow path to adjust pressure in the analysis flow path to a predetermined pressure; a small-diameter pipe connected to the outlet of the back-pressure control valve, having an inner diameter allowing internal pressure to be maintained at a pressure higher than the atmospheric pressure; a large-diameter pipe connected to a downstream end of the small-diameter pipe, having a larger inner diameter than the small-diameter pipe; and a large-diameter pipe heating part for heating the large-diameter pipe.