A sample management device which comprises a source flow path in which a fluidic sample can flow, a volume flow adjustment unit configured for adjusting a volume flow of the fluidic sample to be branched off from the source flow path at a fluidic coupling point, and a fluidic valve fluidically coupled with the source flow path and with the volume flow adjustment unit, wherein the fluidic valve is switchable into a branch off state in which the fluidic coupling point is established within the source flow path to branch off an adjustable volume of the fluidic sample from the source flow path via the fluidic coupling point while a flow of the fluidic sample in the source flow path continues.

Examples of gas liquid separators include a chamber, a fluid mixture inlet, a gas outlet and a liquid outlet. The fluid mixture inlet and the gas and liquid outlets are in fluid communication with the chamber. A fluid mixture received at the fluid mixture inlet diffuses inside the chamber and is separated into a liquid and a gas. The separated liquid is gravity-fed to the liquid outlet. The gas liquid separators have reduced dispersion and increased liquid recovery in comparison to conventional gas liquid separators used for chromatographic separations. The reduced dispersion yields an improvement in the shape of chromatographic peaks.

In a sample separation apparatus for separating a fluidic sample using a mobile phase provided from at least one mobile phase container, a method of determining a density of the mobile phase includes determining a weight and volume reduction behavior according to which weight and volume of mobile phase in a mobile phase container are reduced during conveying mobile phase from the mobile phase container in the sample separation apparatus. The density of the mobile phase is determined based on the determined weight and volume reduction behavior.

The liquid feeding device includes a discharge speed calculation part configured to calculate a discharge speed v of the plunger pump so that a converted value L.sub.ATM under an atmospheric pressure of a flow rate L.sub.PRE of the mobile phase discharged to the discharge channel from the pump part becomes a set flow rate L.sub.SET using a volume V of the mobile phase in the pump chamber of the closing pump immediately before the precompression process is started, a volume V reduced due to the precompression process of the mobile phase in the pump chamber of the closing pump, and a feeding pressure P; and a discharge operation controller configured to control a discharge speed of the plunger pump to a discharge speed v calculated by the discharge speed calculation part.

A method of controlling the liquid or gas flow disturbance impulses in a system for the extraction of targeted constituents from a liquid or gas through the coordination of the acceleration of the valves and pumps in such a way that the fluid pulsations and impact dynamics that cause the destruction of the internal is minimized through a reduction of hydrodynamic pulses propagated through the system.

A manifold assembly has a manifold and can be located between a block and a plate, and serve as a stator for a valve or other component. The manifold, block or plate can have a plurality of ports either integral thereto or removably connected thereto, with the ports adapted to receive and sealingly engage with a tube having an inner tube layer, an outer tube layer, a sleeve, a tip portion, and a nut. Each of the nut, sleeve, inner and outer tubing layers, and tip portion have a passageway therethrough, with at least the passageways in the sleeve, tip portion, outer tube layer, and nut adapted to allow the inner tube layer to pass therethrough or extend over the inner layer. The ends of the tip portion and inner layer together can define a substantially flat surface which can form a seal in a flat-bottomed port of the manifold, block, or plate, which can be used in a component in an analytical instrument system, including for example a liquid chromatography system. The nut, tube, ferrule, and transfer tube or liner tube may comprise biocompatible materials. In addition, the nut may have a slot, such as a slot adapted to allow the tube and the nut to be easily and quickly separated or to allow a portion of the tube to be easily and quickly inserted in the nut.

A liquid chromatography monitoring system comprises a computer or electronic controller comprising computer-readable instructions operable to: (a) draw a fluid into a syringe pump; (b) configure a valve so as to fluidically couple the pump to either a fluidic pathway through a fluidic system or to a plug that prevents fluid flow; (c) cause the syringe pump to progressively compress the fluid therein or expel the fluid to the fluidic pathway, while measuring a pressure of the fluid; (d) determine a profile of the variation of the measured pressure; (e) compare the determined profile to an expected profile that depends upon the fluid; and (f) provide a notification of a sub-optimal operating condition or malfunction if the determined profile varies from the expected profile by greater than a predetermined tolerance.

The present invention addresses the problem of providing a novel method for the pretreatment of a biological sample containing lenalidomide enantiomer and thereby establishing a simple and accurate method for the quantitative analysis of lenalidomide enantiomer. In the present invention, the racemization and decomposition of lenalidomide enantiomer in a biological sample can be prevented by the deproteinization under acidic conditions of the biological sample containing lenalidomide enantiomer, and the lenalidomide enantiomer can be simply and accurately quantitatively analyzed by subjecting to HPLC the biological sample that has been pretreated in such a way.

Exemplary embodiments are directed to a gas liquid separator that includes a chamber, a fluid mixture inlet, a solvent outlet and a gas outlet. The gas liquid separator can include a phase-change inducing mechanism disposed in or proximate to the fluid mixture inlet. Exemplary methods of improving separation of a fluid mixture in a gas liquid separator and CO.sub.2-based chromatography flow systems including a gas liquid separator are also provided.

The present invention relates to the field of RNA analysis. In particular, the invention concerns the use of a catalytic nucleic acid molecule for the analysis of an RNA molecule. The invention concerns methods for analyzing the 5 terminal structures of an RNA molecule having a cleavage site for a catalytic nucleic acid molecule. In particular, the invention concerns a method for determining the presence of a cap structure in an RNA molecule having a cleavage site for a catalytic nucleic acid molecule, a method for determining the capping degree of a population of RNA molecules having a cleavage site for a catalytic nucleic acid molecule, a method for determining the orientation of the cap structure in a capped RNA molecule having a cleavage site for a catalytic nucleic acid molecule and a method for determining relative amounts of correctly capped RNA molecules and reverse-capped RNA molecules in a population of RNA molecules, wherein the population comprises correctly capped and/or reverse-capped RNA molecules that have a cleavage site for a catalytic nucleic acid molecule. Moreover, the present invention provides uses of a catalytic nucleic acid molecule.