The present invention provides a process for preparation of compositions comprising novel mogrosides from fruit of Siraitia grosvenorii. The compositions have superior organoleptic properties compared to known mogroside compositions and are useful in wider range of consumables including foods and beverages.

The present invention relates to a method of preparing a population of antibodies, whereby a desired high-purity and high-quality population of antibodies can be prepared by removing impurities without using an expensive protein A column, and in particular, production costs can be significantly reduced while achieving process automation; and a population of antibodies prepared thereby.

A method for separating substantially pure rare earth metals and other metals from a mixed source, including putting a plurality of rare earth metals and other metals into solution to define a solution containing a plurality of respective metal ions, in at least one chromatographic column, selectively capturing ions of each respective metal with a respective ligand to define a plurality of respective discrete bands, and respectively eluting captured ions of respective metal from each respective band of the at least one chromatographic column to yield a plurality of purified solutions, each respective purified solution having a high concentration of a respective metal. The bands may either be stationary with respect to the columns, or may move through the columns.

The present invention provides an analytical method for separating and optionally quantifying two or more buffers or excipients in a sample in a single assay.

A sample separation device for separating a fluidic sample includes a fluid drive for driving a mobile phase and the fluidic sample when injected in the mobile phase, a sample separation unit for separating the fluidic sample in the mobile phase, and a control unit configured for bracketing the fluidic sample between two mobile phase portions of the mobile phase. At least one of the mobile phase portions is arranged directly next to the fluidic sample and has a higher solvent strength compared to a solvent of the fluidic sample.

The degassing device includes a degassing flow path, a vacuum chamber, a vacuum pump, an inlet flow path, an outlet flow path, a drain flow path, a downstream side switching unit, and a controller. The degassing flow path is made of a gas-permeable, liquid-impermeable tube, and is accommodated in the vacuum chamber. The inlet flow path is for introducing a mobile phase to the degassing flow path, and the outlet flow path is for causing a mobile phase which has passed through the degassing flow path to flow out. The drain flow path is provided separately from the outlet flow path and is configured to drain the mobile phase in the degassing flow path from the degassing flow path. The downstream side switching unit is configured to switch the downstream end of the degassing flow path so as to be connected to either the outlet flow path or the drain flow path. The controller controls the operation of the downstream side switching unit, and is configured to connect the downstream end of the degassing flow path to the drain flow path at a predetermined timing when feeding of the mobile phase by the liquid feeding pump is stopped to drain the mobile phase in the degassing flow path through the drain flow path.

A method for separating at least two metals from each other in a metal refining process, the method comprising: injecting a feed solution comprising the metals into a column or flow pipe comprising a monolithic solid body having a plurality of channels; and flowing the feed solution through the plurality of channels in the monolithic solid body to separate the metals.

An object of the present invention is to suppress the variation of the elution position of a compound having a charged portion by a preservation liquid, in the purification of the compound, without carrying out the substitution step of the preservation liquid attached to the adsorbent used for the purification and the keeping step. A method for purifying a compound having a charged portion, the method comprising the steps of: preparing a composition containing a compound having a charged portion; preparing a buffer solution comprising a buffering agent and an alcohol, the buffer containing a calcium phosphate compound at least partially, having a buffer capacity in a range of pH 6.0 to pH 8.0, and being soluble in a polar solvent and insoluble in a non-polar solvent; preserving an adsorbent in the buffer solution; adsorbing the compound on the adsorbent by bringing the composition into contact with the adsorbent preserved in the buffer solution; and separating the compound from the adsorbent by gradient elution.

A method for the separation or depletion of empty AAV capsids from full AAV capsids in an aqueous mixture comprising empty and full AAV capsids, wherein the mixture is contacted with a primary amino groups bearing solid phase surface in a first alkaline milieu whereby (i) full AAV capsids bind to the solid phase surface whereas empty AAV capsids at least partially do not bind to the solid phase surface,
or (ii) both full and empty AAV capsids bind to the solid phase surface, and subsequently the empty AAV capsids are at least partially eluted by means of a second alkaline milieu of a pH value higher than the pH value of the first alkaline milieu, with the proviso that the second alkaline milieu does not elute full AAV capsids from the solid phase surface.

A system for preparing solutions for chromatography application is disclosed. The system comprises a T-joint for preparing a buffer solution by mixing at least one first solution and a second solution. The T-joint receives the second solution from a solution supply unit connected to the T-joint. Further one or more low pressure pumps supply the one or more first solutions into the T-joint. The high pressure pump collects the buffer solution and delivers it to a chromatography apparatus.