The present invention relates to a preparative chromatography system (200, 500, 800) and a chromatography process (400, 700) adapted to repetitive cycling of chromatography volumes. The system (200, 500, 800) comprises at least two upstream pumps (203a, 803a, 203b, 803b) and separate flow paths (220) from process liquid sources to the chromatography device (200, 500, 800). The system (200, 500, 800) is arranged to prime one flow path (220) with one process liquid while providing another process liquid to the chromatography device and thereby minimizing the hold-up volume of the system (200, 500, 800).

An object of the present invention is to suppress the variation of the elution position of a compound having a charged portion by a preservation liquid, in the purification of the compound, without carrying out the substitution step of the preservation liquid attached to the adsorbent used for the purification and the keeping step. A method for purifying a compound having a charged portion, the method comprising the steps of: preparing a composition containing a compound having a charged portion; preparing a buffer solution comprising a buffering agent and an alcohol, the buffer containing a calcium phosphate compound at least partially, having a buffer capacity in a range of pH 6.0 to pH 8.0, and being soluble in a polar solvent and insoluble in a non-polar solvent; preserving an adsorbent in the buffer solution; adsorbing the compound on the adsorbent by bringing the composition into contact with the adsorbent preserved in the buffer solution; and separating the compound from the adsorbent by gradient elution.

Provided herein are, inter alia, biological manufacturing and downstream purification processes.

A regeneration solvent comprised of one or more ethylene amines may contact an adsorbent bed that has been used to remove sulfur compounds from a hydrocarbon stream to extract adsorbed sulfur compounds from the adsorbent material in the bed to regenerate it. The one or more ethyleneamines may have structure (I), (II), or (III): ##STR00001##
where R.sup.1, R.sup.2, R.sup.5 and R.sup.6 are, to the extent chemically possible, independently H, C.sub.1-C.sub.4 linear or branched alkyl, amido (RRNC═O), or hydroxyalkyl, where each R in the amido group is independently H or C.sub.1 alkyl, where R.sup.3 and R.sup.4 are alkylene of from 1 to 4 carbon atoms, where x ranges from 0 to 3, y ranges from 1 to 6. The regenerated adsorbent bed may be reused, either alone or in combination with a liquid-liquid extraction column, to remove sulfur compounds from a hydrocarbon stream.

Apatite pretreatment methods are provided. The method is applied to the apatite solid surface prior to first chromatographic use. In one embodiment, the method may be achieved by contacting an apatite solid surface with a phosphate buffered solution at a pH of at least about 6.5 and contacting the apatite solid surface with a solution having a hydroxide.

Disclosed is a chromatographic method for separating and purifying a recombinant human fibronectin from a genetically engineered rice seed that expresses the human fibronectin. In the method, the genetically engineered rice seed is milled, mixed with an extraction buffer, and then filtered to obtain a crude extract comprising the recombinant human fibronectin; the crude extract comprising the recombinant human fibronectin is subjected to cation exchange chromatography, so as to perform primary separation and purification, thereby obtaining a primary product comprising the recombinant human fibronectin; and the primary product is subjected to anion exchange chromatography so as to perform final separation and purification to obtain the recombinant human fibronectin as a target substance. The method is low cost and easily utilized on an industrial scale. The obtained OsrhFn target substance has a SEC-HPLC purity greater than 95% with excellent bioactivity.

Methods, sorbent cartridges and cleaning devices are disclosed for refurbishing sorbent materials. In one implementation among multiple implementations, a medical fluid delivery method includes: providing a sorbent cartridge including H.sup.+ZP within a casing for a treatment; and after the treatment, refurbishing the H.sup.+ZP while maintained within the casing via (i) regenerating the non-disinfected H.sup.+ZP by flowing an acid solution through the casing, (ii) rinsing the regenerated H.sup.+ZP while maintained within the casing, (iii) disinfecting the regenerated and rinsed H.sup.+ZP by flowing a disinfecting agent through the casing, and (iv) rinsing the regenerated and disinfected H.sup.+ZP while maintained within the casing. Multiple batch sorbent refurbishing implementations are also disclosed.

Provided are methods for determining the apolipoprotein E (ApoE) phenotype in a sample by mass spectrometry; wherein the ApoE allele(s) present in the sample is determined from the identity of the ions detected by mass spectrometry. In another aspect, provided herein are methods for diagnosis or prognosis of Alzheimer's disease or dementia.

High resolution affinity chromatography combining affinity resolving and affinity capture processes using a single chromatography matrix results in improved resolution between closely related molecular species and significantly enhances overall product yield for large scale commercial production of heterodimeric proteins such as bispecific antibodies. Moreover, tankage and equipment requirements are reduced via the ability to reduce salt concentration, while increasing product purity and concentration, without the need for dilution, ultrafiltration or diafiltration.

Method for manufacturing an inert solid support with optionally functionalised carbon nanotubes (CNTs), comprising the steps of: i) providing an inert solid support and at least one catalytic metal associated with, or absorbed in, or adsorbed/deposited on, said support, said metal being optionally selected from among the group consisting of iron, cobalt, nickel, molybdenum and combinations thereof; ii) supplying a source of gaseous, liquid or solid carbon to the catalytic metal; iii) through chemical vapor deposition (CVD), depositing at least part of the carbon source at the catalytic metal as CNTs, stably connected to the inert solid support. The present invention further regards an inert solid support and a separation method.