An ion exchange chromatographic packing material is described that includes support resin particles and a copolymer grafted to the support resin particles. The copolymer includes polymerized functional monomers such as a first ion exchange group monomer and a second ion exchange group monomer. At a first pH, the first ion exchange group monomer is configured to have a first charge at a first pH, and the second ion exchange group monomer is configured to have a net neutral charge. At a second pH, the first ion exchange group monomer is configured to have the first charge at a second pH, and the second ion exchange group monomer is configured to have a second charge at the second pH where the first charge and second charge both have a same polarity.

This disclosure provides chromatography columns that are packed with incompressible media such as ceramic hydroxyapatite particles, which exhibit high separation performance that is robust to transportation and multiple uses. The columns can be made by applying axial compression using rigid bodies, such as porous frits and/or flow regulators.

A chromatographic cassette includes a cassette including a chamber, chromatographic media disposed within the cassette chamber, a distribution network fluidly coupled to the chromatographic media and an inlet port and an outlet port coupled to the distribution network. A hyper-productive chromatography technique includes providing a scalable and stackable chromatographic cassette, loading a sample to be processed, operating the scalable chromatographic cassette having an adsorptive chromatographic bed having a volume greater than 0.5 liter by establishing a flow at a linear velocity greater than 500 cm/hr with a residence time of the loading step of less than one minute.

If one sterilizes pre-packed, plastic chromatography columns with an appropriate level of gamma irradiation, the resulting sterile chromatography columns maintain sufficient packing media function and maintain column mechanical properties and pressure ratings.

A slurry chamber is fastened to and held in coaxial alignment with a chromatography column by a V-band. One end of the column has an end cap and frit. A slurry of fluid and chromatographic media is placed in the column and chamber. A piston is forced through the chamber and into the column by a hydraulic ram to expel the fluid and form a media bed in the column. The slurry chamber is removed, and a split end cap is fastened to the column. A threaded recess in the split end cap receives a split, threaded collet. The collet is tightened until it pushes against the piston with the same force as the ram, at which point the ram is released and the column removed for use.

A slurry chamber is fastened to and held in coaxial alignment with a chromatography column by a V-band. One end of the column has an end cap and frit. A slurry of fluid and chromatographic media is placed in the column and chamber. A piston is forced through the chamber and into the column by a hydraulic ram to expel the fluid and form a media bed in the column. The slurry chamber is removed, and a split end cap is fastened to the column. A threaded recess in the split end cap receives a split, threaded collet. The collet is tightened until it pushes against the piston with the same force as the ram, at which point the ram is released and the column removed for use.

Cartridges useful in regenerating or purifying dialysis solutions are described as well as methods to regenerate or purify spent dialysis solutions. Dialysis methods using the sorbent cartridges of the present invention are further described.

Particle loading assembly and method for loading particles into a vessel to form a densely packed particle bed comprising an inner layer of particles and an outer layer of particles. The inner layer of particles is arranged radially and concentric with the outer layer of particles. The inner layer of particles contains at least a first type of particle of different granulometry or range of composition or both granulometry and range of composition from a second type of particles contained in the outer layer.

The invention discloses a piston for a process column, said piston comprising a top side and a bed contact side, wherein said top side comprises a drainage trench.

A method and a system for transferring a slurry of a separation resin, from at least one first container to a second container, said method comprising the steps of: connecting the at least one first container to the second container by a transferring connection; providing a degree of vacuum to the second container by a vacuum production device connected to the second container; allowing the vacuum in the second container to affect the content in the at least one first container through the transferring connection such that the slurry initially provided in the at least one first container is transferred to the second container through the transferring connection.