A method and a system and a container system for transferring separation resin from at least one first container (3; 3; 3a, 3b; 3a, 3b, 3c, 3d) to a second container (5; 5), wherein said first container is a deformable, single-use separation resin storage container, said method comprising the steps of:preparing (S1) the at least one first container by providing a deformable, single-use container comprising an outlet port (4) with a predefined volume of separation resin in a storage solution;fluidizing (S3) the separation resin in the at least one first container to provide a resin slurry, said fluidizing being performed by mechanical interaction to the first container from an outside of the first container to provide a deformation of said first container;fluidically connecting (S5) the outlet port (4) of the at least one first container to an inlet port (103) of the second container;transferring (S7) separation resin from the at least one first container to the second container by generating a pressure difference between an interior of the second container and an interior of the first container where the pressure is lower in the second container.

The present invention relates to a method for sterilizing a chromatography material, more particularly microporous polymer membranes, to a chromatography material which has been sterilized by the method according to the invention, to the use of the chromatography material sterilized according to the invention for the chromatographic removal of contaminants from a fluid, and to a closed chromatographic filtration system comprising a chromatography material sterilized according to the invention.

A method for packing a media bed in a column (3). According to the invention the method comprises the steps of: providing data to a control unit (15) connected to the column (3), said data comprising at least a measured slurry concentration, a target bed height, a target packing factor or compression factor and minimum and maximum acceptable values for at least one of target bed height and target packing or compression factor; forcing a movable adapter (9) along a longitudinal axis of the column (3) in order to pack the media; detecting when the media bed is consolidated; the control unit (15) processing the provided data and the information about the consolidated bed height in order to present to the user acceptable bed heights, if there are any, giving acceptable packing or compression factors.

The invention provides a purification column that contains an adsorbent and shows excellent adsorptivity. Specifically, this purification column is provided with an adsorbent and a case that contains the adsorbent and has a tubular housing and treatment fluid supply port and discharge port at both ends of the housing. The purification column is characterized in that: a distribution plate is disposed at least on one end face side of the adsorbent; the distribution plate has a plurality of openings that allow communication of the treatment fluid, a support body, and a protrusion extending from the supporting body to the adsorbent side; and at least part of the protrusion is inserted into the adsorbent.

The invention provides superficially porous metal oxide particles with precisely controlled particle density and to methods for their preparation and use, as well as to separation devices (e.g., high pressure liquid chromatography) having superficially porous particles.

A hyper-productive chromatography technique includes providing a scalable and stackable chromatographic cassette, loading a sample to be processed, operating the scalable chromatographic cassette having an adsorptive chromatographic bed having a volume greater than 0.5 liter by establishing a flow at a linear velocity greater than 500 cm/hr with a residence time of the loading step of less than one minute.

If one sterilizes pre-packed, plastic chromatography columns with an appropriate level of gamma irradiation, the resulting sterile chromatography columns maintain sufficient packing media function and maintain column mechanical properties and pressure ratings.

The present invention relates to axial flow chromatography columns, methods for separating one or more analytes in a liquid by the use of such columns, and systems employing such columns. The column comprises a first port and a second port, the first port and said second port being at essentially the same level or elevation above the level of the bed space on the chromatography column.

In the specification, a method for purifying a compound by column chromatography, and a method for optimizing the symmetry factor and theoretical plate number by allowing the slurry to settle and reducing the sedimentation volume of the slurry. A compound purified by such method is also provided.

A column filling apparatus mainly includes a tank, a stirrer being an agitator, a pump, a resin bottle, first to eleventh pipes, first to fourth valves composed of three-way valves, and fifth and sixth valves composed of two-way valves. A suction port is mounted on a leading end of the fifth pipe, and the suction port is inserted into an inner part from a top opening of the resin bottle. The other end of the fifth pipe is connected to the first valve. The first valve is connected to the first pipe, the second pipe, and the fifth pipe, and connects or disconnects these pipes to or from each other. An end of the first pipe is mounted near a bottom of the tank, and is opened near an inner bottom surface of the tank.