A method of recovering paraxylene in a pressure swing adsorption unit with varying hydrogen purge pressures. The pressure swing adsorption zone is adapted to adsorb and desorb paraxylene based on the cycling of partial pressure in the zone. A first hydrogen purge is fed concurrent to the feed. A second hydrogen purge is countercurrent to the feed.

A method of recovering paraxyiene in a pressure swing adsorption unit with varying hydrogen purge pressures. The pressure swing adsorption zone is adapted to adsorb and desorb paraxyiene based on the cycling of partial pressure in the zone. A first hydrogen purge fed to the zone is within 50 psi of the adsorption pressure of paraxyiene in the zone. A second hydrogen purge fed to the zone is within 50 psi of the desorption pressure of paraxyiene in the zone. The overall amount of hydrogen necessary to operate the pressure swing adsorption zone is reduced and heat may be recovered from the effluent leaving the pressure swing adsorption zone.

A method of recovering paraxyiene in a pressure swing adsorption unit with varying hydrogen purge pressures. The pressure swing adsorption zone is adapted to adsorb and desorb paraxyiene based on the cycling of partial pressure in the zone. A first hydrogen purge fed to the zone is within 50 psi of the adsorption pressure of paraxyiene in the zone. A second hydrogen purge fed to the zone is within 50 psi of the desorption pressure of paraxyiene in the zone. The overall amount of hydrogen necessary to operate the pressure swing adsorption zone is reduced and heat may be recovered from the effluent leaving the pressure swing adsorption zone.

A chromatography system is provided. The chromatography system is configured to process a feed fluid containing a plurality of components, wherein at least one component of the plurality of components of the feed fluid is a target component. The chromatography system comprises: a flow path comprising a plurality of fluid control components configured to control a fluid flow; a stationary phase, wherein the stationary phase is at least one membrane adsorber connected to the flow path and the stationary phase is configured to isolate the target component. The flow path is configured such that harvesting of the target component is optimized.

The invention concerns a purification method comprising: providing a flow comprising a glycol, monovalent ions and multivalent ions; treating this flow with ion exclusion chromatography comprising: injecting the flow into a chromatographic unit comprising an ion exchange stationary phase; injecting an eluent into the chromatographic unit; collecting a fraction at the outlet of the chromatographic unit; the collected fraction being enriched with glycol and depleted of monovalent ions and multivalent ions relative to the flow. The invention also concerns an installation adapted to implement this method, and its application to the regeneration of an anti-hydrate agent.

A fluidic valve for switching between different fluid coupling states includes a stator having at least one fluidic stator interface, a rotor having at least one fluidic rotor interface, wherein the rotor is rotatable relative to the stator to thereby switch the fluidic valve between a plurality of different fluid coupling states between the at least one fluidic stator interface and the at least one fluidic rotor interface, and a force transmission mechanism configured for pressing the stator and the rotor together by a contactless force transmission to provide for a fluid tight sealing between the stator and the rotor.

The present invention is in the field of purification and protein purification in particular. The invention provides improved techniques for the industrial-scale purification of proteins and other biomolecules. More specifically, it relates to a process for the purification of a compound of interest, such as a protein, preferably an antibody or an antibody fragment using a chromatography step, preferably a semi-continuous chromatography step.

The invention is a method for liquid, gaseous or supercritical phase chromatography which involves circulating, on a chromatograph (6) containing a stationary phase, a load (1) comprising components to be separated entrained by a carrier fluid (2), said method being characterized in that it involves: (a) obtaining, at the outlet of the chromatograph, a plurality of chromatographic fractions (3, 4) comprising at least one component of the load and the carrier fluid in a first fluid phase, (b) imposing a change of state on at least one of said chromatographic fractions (3, 4) so as to obtain at least one fraction of purified carrier fluid in a second fluid phase different from the first fluid phase by separating said carrier fluid from the component of the load, (c) imposing a change of state in a reverse direction to that of step (b) on at least one fraction of purified carrier fluid obtained in step (b) so as to obtain at least one fraction of purified carrier fluid in a third fluid phase different to the second fluid phase, and in that it involves coupling the change-of-state energies from the first fluid phase to the second fluid phase and from the second fluid phase to the third fluid phase of the same or of another fraction of purified carrier fluid, said coupling comprising a transfer of heat using a heat pump.

The present disclosure relates to an enhanced multi-dimensional chromatography system and method using selectable At-Column Dilution to improve compatibility of the interface and transfer between the multiple dimensions. The use of At-Column Dilution (ACD) with multi-dimensional chromatography can provide greater retention of the diverted components on subsequent stationary phases, and increase the sensitivity and peak shape of the component(s) separated on subsequent dimensions.

The present invention relates to a method for performing liquid chromatography purification of one or more target molecules from a sample comprising: providing an eluent flow having one or more target molecules, measuring an output parameter indicative of the content of the one or more target molecules in the eluent flow, storing output parameter data, and dividing the eluent flow into consecutive eluent fractions, dividing the output parameter data into corresponding data fractions, in each data fraction obtaining a value indicative of characteristic behavior of the measured output parameter, identifying trends in the measured output parameter based upon the obtained value in consecutive data fractions, and identifying peak(s) in the measured output parameter correlated to eluent fractions based upon the identified trends, whereby information of identified peak(s) and correlated eluent fraction(s) can be presented and purified sample(s) from the eluent may be collected.