The present disclosure relates to a method of separating a compound of interest, particularly unsaturated compound(s) of interest, from a mixture. The compound is separated using a column having a chromatographic stationary phase material for various different modes of chromatography containing a first substituent and a second substituent. The first substituent minimizes compound retention variation over time under chromatographic conditions. The second substituent chromatographically and selectively retains the compound by incorporating one or more aromatic, polyaromatic, heterocyclic aromatic, or polyheterocyclic aromatic hydrocarbon groups, each group being optionally substituted with an aliphatic group. In some examples, the present disclosure can include a chromatographic system having a chromatographic column having a stationary phase with a chromatographic substrate containing silica, metal oxide, an inorganic-organic hybrid material, a group of block copolymers, or a combination thereof.

Provided herein in some embodiments is a column including a housing assembly and a cartridge assembly. The housing assembly can include a housing top, a housing bottom, and a housing siding. The housing siding can be fixedly coupled to the housing top and the housing bottom forming hermetic seals therebetween. The cartridge assembly can include a cartridge top, an outer frit, and an inner frit disposed within the outer frit. A toroidal space can defined by the cartridge top, the outer frit, the inner frit, and the housing bottom. The toroidal space can be configured to hold a stationary phase for radial flow column chromatography. Also provided herein in some embodiments is a process including assembling the cartridge assembly, assembling the housing assembly about the cartridge assembly, and pressure testing the column. In some embodiments, the process can further include charging the toroidal space with a stationary phase.

The invention relates to a method for the chromatographic purification of at least one cannabinoid compound, wherein the method comprises a main purification stage comprising the steps of: injecting an initial mixture comprising the at least one cannabinoid compound and one or more additional compounds onto a main stationary phase comprising silica particles, the silica particles comprising amino and/or diol groups; performing an elution with an elution solution, and collecting one or more elution fractions; and optionally, washing the main stationary phase with a washing solution and collecting one or more washing fractions; at least one of the elution fractions or washing fractions containing the at least one cannabinoid compound purified from the one or more additional compounds.

A method for obtaining .sup.225AC from .sup.225Ra having the steps of assembling a column having an inorganic stationary phase; priming the column to immobilize .sup.226Ra .sup.225Ra and natural decay products therefrom; immobilizing the .sup.226Ra, .sup.225Ra, .sup.224Ra, and natural decay products therefrom onto a stationary phase within the column; and eluting the column containing the .sup.225Ra with an aqueous sulfate solution to obtain a milking effluent that contains .sup.225AC. Also provided is a method for obtaining pure .sup.225AC from its isotope parents, the method comprising assembling a column having a stationary phase comprising an inorganic material; priming the column with the isotope parents to immobilize .sup.225Ac, and natural decay products of .sup.225AC; immobilizing the .sup.225Ac, and natural decay products therefrom onto the stationary phase within the column .sup.226Ra, .sup.225Ra, .sup.224Ra; and eluting the column containing the .sup.225AC to obtain an effluent that contains the isotope parents.

The invention provides columns (including pipette tip columns) and automated methods for the purification of nucleic acids including plasmids. Nucleic acids can be purified from unclarified, clarified or partially-clarified cell lysates that contain cell debris. The columns typically include a bed of medium positioned above a bottom frit and with an optional top frit. Plasmid preparation scales include miniprep, midiprep, maxiprep, megaprep and gigaprep.

A method is provided for removing THC from raw cannabis oil. Additionally, new compositions of cannabis oil are provided. Further, a new method of obtaining a substantially pure cannabinoid is provided

A method is provided for removing THC from raw cannabis oil. Additionally, new compositions of cannabis oil are provided. Further, a new method of obtaining a substantially pure cannabinoid is provided.

The invention relates to a method for controlling preparative liquid chromatography, comprising the following steps, at least a part of said steps being implemented by a computer comprising a processor and a display screen coupled to said processor: (a) selecting an analytical liquid chromatography method from among thin layer chromatography (TLC) and high performance liquid chromatography (HPLC), (b) inputting analytical liquid chromatography data obtained by the method selected at step (a) for a product to be purified, (c) accessing a table of separating tools available to the user to implement said preparative liquid chromatography, (d) from said analytical liquid chromatography data and table of available separating tools, selecting an optimal separating tool from said table and computing preparative liquid chromatography operating conditions for said selected separating tool.

The subject of the invention is a process for the preparation of high purity prostaglandin acid of the general formula II wherein the bonds marked with dotted lines represent single or double bonds wherein the double bonds may be cis- or trans oriented, Y represents O or CH.sup.2, and R.sup.3 stands for a phenyl group which is optionally substituted with CF.sub.3, wherein the crude prostaglandin acid of the general formula II is purified by normal phase silicagel chromatography. ##STR00001##

A method is provided for removing THC from raw cannabis oil.