Various aspects described herein relate to electrochemical devices, e.g., for separation of one or more biomolecules from a solution, and methods of using the same. Methods for using the electrochemical devices for biocatalysis are also described herein.

A portable fluidic platform for rapid and flexible end-to-end production of recombinant protein biologics includes a bioreactor system hosting stable and robust cell-free translation systems that is fluidically integrated with modular protein separation functionalities (e.g., size exclusion, ion exchange or affinity chromatography systems) for purification of the cell-free expressed product and which are configurable for process-specific isolation of different proteins, as well as for formulation. The bioreactor utilizes lysates from engineered eukaryotic (e.g., yeast) or prokaryotic (e.g., bacterial) strains that contain factors for protein folding and posttranslational modifications. Combination of various purification modules on the same fluidic platform allows flexibility of re-routing for purification of different proteins depending on specific target requirements. Protein synthesis and purification modules are integrated into self-contained disposable fluidic cartridge that eliminates cross-contamination between runs. The platform allows for flexible production of protein biologics within 24 hours (from DNA to purified product).

Devices and methods suitable for cell separation. The devices herein include non-random voids interconnected through non-random pores and/or non-random solid geometrical structures optionally connected through solid non-random interconnecting elements. Such devices are preferably suitable for affinity-based cell isolation techniques which rely upon binding interactions.

The disclosure provides systems and methods useful for predicting or detecting a malfunction in a chromatography process in real-time. In some embodiments, the disclosure provides systems and methods for detecting an atypical profile in a process chromatogram in ion-exchange chromatography of a biologic product.

The present disclosure relates to multistep chromatographic methods for preparing extracellular vesicles (EVs). The methods were demonstrated to be effective in preparing high quality EVs in a large scale. The methods enable preparation of EVs for therapeutic and diagnostic applications, and isolation and/or sub-fractionation of EVs with desired properties for specific use.

Methods of producing a CBD/THC oil are disclosed. In some embodiments, the method may include extracting CBD/THC from plant matter using one or more solvents, winterizing the solvent extract, and evaporating the one or more solvents from the winterized extract. The method may additionally include distilling the evaporated extract via a short path distillation apparatus to produce an initial distillate oil, mixing the initial distillate oil with at least one solvent, and running the mixture of initial distillate oil and at least one solvent through a chromatography column to produce an effluent. The method may further include evaporating the at least one solvent from the effluent, distilling the evaporated effluent via a short path distillation apparatus to produce a final distillate oil, and mixing one or more desired terpenes with the final distillate oil.

The present disclosure relates to nucleic acid extraction and purification methods and devices to accomplish the same.

Herein is reported a fusion polypeptide according to formula I (TAG-X1-C1qA-X2-C1qB-X3-C1qC-X4), comprising a fragment of SEQ ID NO: 01 (C1qA), a fragment of SEQ ID NO: 03 (C1qB), a fragment of SEQ ID NO: 05 (C1qC) and optionally a tag (TAG).

To provide a porous particle with which non-specific adsorption is hardly generated although the porous particle is a synthetic polymer-based particle, the mechanical strength is high, and the dynamic binding capacity is high in a case where a ligand is bound to the porous particle; and a method for producing the same. A method for producing a porous particle, including the following steps 1 and 2; (step 1) dissolving at least one or more of polymers selected from the group consisting of a vinyl alcohol polymer and an ethylene-vinyl alcohol copolymer in an aqueous solvent to prepare a polymer solution; and (step 2) dispersing the polymer solution in a non-aqueous solvent to form a W/O emulsion.

The present invention provides novel chromatographic materials, e.g., for chromatographic separations, processes for their preparation and separations devices containing the chromatographic materials. The preparation of the inorganic/organic hybrid materials of the invention wherein a surrounding material is condensed on a superficially porous hybrid core material will allow for families of different hybrid packing materials to be prepared from a single core hybrid material. Differences in hydrophobicity, ion-exchange capacity, chemical stability, surface charge or silanol activity of the surrounding material may be used for unique chromatographic separations of small molecules, carbohydrates, antibodies, whole proteins, peptides, and/or DNA.