Disclosed are multi-enzyme biosensors that are stable at ambient temperature, and methods of making thereof.

The subject of the invention is a membrane for separation of target stem cells from biological samples, more precisely from a single-cell suspension that was prepared from a biological sample. As a result, sterile target stem cells are obtained in physiological buffer. The membrane of the invention consists of a 3D carrier structure made of at least one layer of biocompatible polymer with specific pore size, as a carrier material, and covalently bound target molecules on its surface and/or in the pores. These target molecules are preferably target antibodies, which recognize characteristic antigens that are bound on the surface of the target stem cells and thus bind the target stem cells to the membrane. Target molecules can be either directly bound to the surface and/or in the pores of the carrier structure or are bound to the surface and/or in the pores of the carrier structure through specific functionalized nanoparticles, which are bound to or embedded into the 3D carrier structure of the membrane. In addition, the present invention includes the membrane production process as well as the process and device for the separation of target stem cells from a biological sample, which includes the above membrane as a constituent part.

A nanobiocatalytic membrane for a filtration system is provided which includes a filtration membrane and a plurality of nanobiocatalyst nanoparticles associated with the membrane, each of the nanobiocatalyst nanoparticles including a core, a coating at least partially surrounding the core, and a plurality of nanobiocatalysts coupled to the coating. Each of the plurality of nanobiocatalysts includes an antibacterial nanoparticle comprising bismuth, and a quorum quenching agent coupled to the antibacterial nanoparticle. A nanobiocatalyst nanoparticle for use with a water purification system is also provided. A method of forming a nanobiocatalytic membrane for a filtration system and a method of using a nanobiocatalytic membrane in a filtration system are also provided.

The present disclosure describes compositions and methods for preparing membrane protein nanosheets and two-dimensional crystals. In particular, the methods employ a solvent. A mixture of a polymer and a membrane protein is solubilized in the solvent, applied to a substrate, and subsequently dried to form the nanosheet or two-dimensional crystal. Applicants have surprisingly found that the membrane proteins maintain their structure when exposed to solvents during the short processing time utilized.

The present disclosure describes membrane compositions and methods for preparing membrane compositions. In particular, the methods employ a layer-by-layer approach to membrane preparation. The membrane compositions provide significantly enhanced membrane performance over existing commercial membranes, particularly in terms of permeability and selectivity.

Flow capture device and method for removing cells from blood The current invention discloses a blood treating and/or purifying device for removing circulating pathogens, preferably pathogenic cells, more preferably circulating tumor cells from the blood of a patient, a method of producing such a device and method to treat cancer and other diseases caused by virus infection, bacterial infection and parasites infection as well as autoimmune disorders. The described method is an extracorporeal medical therapy, thus can be done also in a hemodialysis system. The current invention also describes a device and an in-situ production method of preparing the device to remove CTC and other pathogens i.e. virus, bacteria or parasites from the bloodstream.

The present invention relates to CO.sub.2 capture from gas mixtures by use of gas separation membranes. In particular, the invention relates to a gas separation membrane comprising: a gas permeable or porous support layer; and at least one CO.sub.2 selective polymer layer comprising carbonic anhydrase (CA) enzymes fixed within the at least one CO.sub.2 selective polymer layer. The present invention also relates to the method of separating CO.sub.2 from a gas and to the use of the gas separation membrane.

Methods of treating an inert surface of a substrate to improve the adherence to the treated surface of micro-dimensioned particles including the steps of: contacting the inert surface with in an aqueous dispersion of a construct of the structure F-S-L; and then washing the surface with an aqueous vehicle to provide the treated surface, where F is a polyamine; S is —CO(CH.sub.2).sub.2CO—, —CO(CH.sub.2).sub.3CO—, —CO(CH.sub.2).sub.4CO— or —CO(CH.sub.2).sub.5CO—; and L is a diacyl- or dialkyl-glycerophospholipid.

A method for processing a fluid with forward osmosis process includes providing one or more tubular membranes each including a tubular nonwoven base layer on the outside of the tubular membrane forming an outer shell of the tubular membrane and providing a lumen for feed flow; a polymer substrate layer on the lumen-side of the tubular membrane comprising three regions, including a region where the polymer substrate layer is partially intruded into the tubular base layer, a region with an open macrovoid structure and a region with an asymmetrical foamy layer, where the partially intruded region forms an intermediate layer; and a functional top layer on the polymer substrate layer. The tubular base layer comprises a longitudinal weld. The method includes providing the feed flow through the lumen and providing a draw solution on the outer shell side of the tubular membrane; and processing the feed flow with the membrane.

A crosslinked protein-based separation membrane and application thereof. The separation membrane is formed by attaching a crosslinked protein nanomembrane to a porous membrane, the crosslinked protein nanomembrane is formed by crosslinking a two-dimensional nanomembrane which is formed by phase transition of a protein with a crosslinking agent, the separation membrane contains a dense surface layer and a support layer, the dense surface layer is the crosslinked protein nanomembrane, and the support layer is the porous membrane; the protein is any one of lysozyme, bovine serum albumin, insulin, and α-lactalbumin; the crosslinked protein-based separation membrane has a good biocompability, may serve as a dialysis membrane for blood purification, and has a higher retention ratio for large molecular proteins.