Disclosed herein are polynucleotides, compositions, and methods for controlling insect pests, especially flea beetles, such as Phyllotreta spp. and Psylliodes spp., particularly in plants. More specifically, polynucleotides such as double-stranded RNA triggers and methods of use thereof for modifying the expression of genes in flea beetles.

Disclosed herein are polynucleotides, compositions, and methods for controlling insect pests, especially flea beetles, such as Phyllotreta spp. and Psylliodes spp., particularly in plants. More specifically, polynucleotides such as double-stranded RNA triggers and methods of use thereof for modifying the expression of genes in flea beetles.

The inventive technology relates to novel paratransgenic strategies for the control of pathogens. The inventive technology may specifically include a novel paratransgenic system configured to deliver one or more inhibitory RNA molecules to pathogen/disease-transmitting organisms. In a preferred embodiment, the invention may include one or more genetically engineered symbiotic bacteria configured to persist throughout the life-cycle of a mosquito and deliver one or more interfering RNA molecules to pathogen/disease-transmitting mosquitoes.

The inventive technology relates to novel paratransgenic strategies for the control of pathogens. The inventive technology may specifically include a novel paratransgenic system configured to deliver one or more inhibitory RNA molecules to pathogen/disease-transmitting organisms. In a preferred embodiment, the invention may include one or more genetically engineered symbiotic bacteria configured to persist throughout the life-cycle of a mosquito and deliver one or more interfering RNA molecules to pathogen/disease-transmitting mosquitoes.

Compositions and methods for reducing or preventing mosquito egg development are provided. Typically, the compositions include an effective amount of a compound that reduces, inhibits, or prevents, expression of a mosquito EOF1, Nasrat, Closca, Polehole Nudel, CATL3, DCE2, DCE4, or DCE5 gene, or a gene product thereof, for example EOF1 or Nudel mRNA or protein. The compound can be a functional nucleic acid such as antisense molecule, siRNA, miRNA, ribozymes, RNAi, or external guide sequences, a gene editing composition, or a protease inhibitor. The disclosed methods typically include contacting mosquito cells with an effective amount of one or more of the disclosed compositions, and can be used to reduce, inhibit, or prevent egg development in an effective number of mosquitoes to reduce transmission of one or more infections or diseases.

Compositions and methods for reducing or preventing mosquito egg development are provided. Typically, the compositions include an effective amount of a compound that reduces, inhibits, or prevents, expression of a mosquito EOF1, Nasrat, Closca, Polehole Nudel, CATL3, DCE2, DCE4, or DCE5 gene, or a gene product thereof, for example EOF1 or Nudel mRNA or protein. The compound can be a functional nucleic acid such as antisense molecule, siRNA, miRNA, ribozymes, RNAi, or external guide sequences, a gene editing composition, or a protease inhibitor. The disclosed methods typically include contacting mosquito cells with an effective amount of one or more of the disclosed compositions, and can be used to reduce, inhibit, or prevent egg development in an effective number of mosquitoes to reduce transmission of one or more infections or diseases.

A topical serum and its method of production and use. The serum is used to treat damage on plant tissue, such as damaged leaves. The serum triggers the immunity responses of the plant, therein enabling the damaged tissue to heal, rather than die and fall away from the plant. The serum is a combination of aqueous solutions. The first solution contains at least one immunity response compound that is selected from methyl jasmonate and methyl salicylate. The second solution contains at least one B vitamin. The solutions are mixed to form a topical serum. The topical serum is applied over the damaged tissue, wherein the immunity response compounds enhance the pattern-triggered immunity response and the effector-triggered immunity response of the plant. The vitamin B solution inhibits the oxidation and preserves undamaged. tissue.

A topical serum and its method of production and use. The serum is used to treat damage on plant tissue, such as damaged leaves. The serum triggers the immunity responses of the plant, therein enabling the damaged tissue to heal, rather than die and fall away from the plant. The serum is a combination of aqueous solutions. The first solution contains at least one immunity response compound that is selected from methyl jasmonate and methyl salicylate. The second solution contains at least one B vitamin. The solutions are mixed to form a topical serum. The topical serum is applied over the damaged tissue, wherein the immunity response compounds enhance the pattern-triggered immunity response and the effector-triggered immunity response of the plant. The vitamin B solution inhibits the oxidation and preserves undamaged. tissue.

A topical serum and its method of production and use. The serum is used to treat damage on plant tissue, such as damaged leaves. The serum triggers the immunity responses of the plant, therein enabling the damaged tissue to heal, rather than die and fall away from the plant. The serum is a combination of aqueous solutions. The first solution contains at least one immunity response compound that is selected from methyl jasmonate and methyl salicylate. The second solution contains at least one B vitamin. The solutions are mixed to form a topical serum. The topical serum is applied over the damaged tissue, wherein the immunity response compounds enhance the pattern-triggered immunity response and the effector-triggered immunity response of the plant. The vitamin B solution inhibits the oxidation and preserves undamaged. tissue.

Provided herein are plants that reduce growth of a fungal pathogen, increase resistance of the plant to a fungal pathogen, or a combination thereof. The plant includes a polynucleotide that reduces expression of a coding region present in a fungal pathogen, such as Sclerotinia sclerotiorum or Botrytis cinerea. The polynucleotide can be present on the surface of the plant, expressed by a plant, or a combination thereof. Also provided are methods of making and methods of using the plants.