Method of analysis. In the method, a first emulsion and a second emulsion substantially separated from one another by a spacer fluid may be formed. The first emulsion, the spacer fluid, and the second emulsion may be flowed in a channel from a fluid inlet to a fluid outlet of a heating and cooling station having two or more temperature-controlled zones, such that each emulsion is thermally cycled to promote amplification of a nucleic acid target in droplets of the emulsion. Amplification data may be collected from individual droplets of each emulsion downstream of the heating and cooling station. A level of the nucleic acid target present in each emulsion may be determined based on the amplification data collected from the individual droplets of the emulsion.

Sometimes, a problem is obvious, everyone sees it, but nothing happens until someone decides to do something useful about it. Methods are herein provided for recovering heavy hydrocarbons from plastic materials and/or geo-formation. In one solution set, PVC waste materials are emulsified by an amine solvent in an aqueous phase, thereby extracting heavier hydrocarbons from the primary structure of PVC into the amine aqueous phase; followed by de-emulsifying the extracted heavier hydrocarbons by separating and recovering the amine solvent, and then separating the de-emulsified heavier hydrocarbons from the aqueous phase by a hydrophobic membrane.

The present invention discloses a method for preparing a micro-channel array plate, comprising the steps of : (1) arranging a first optical fiber glass rod and a second optical fiber glass rod closely, melting the two glass rods into a whole at a high temperature to obtain a melted glass rod, drawing the melted glass rod at least one time into a longer and thinner glass rod than the melted glass rod, and cutting the drawn glass rod into small pieces to obtain a micro-channel array plate blank, wherein the corrosion resistance of the first optical fiber glass rod and the second optical fiber glass rod to the same corrosive liquid is different; (2) corroding the micro-channel array plate blank by a corrosive liquid to obtain a micro-channel array plate crude product with through holes; and (3) conducting hydrophobic treatment on the micro-channel array plate crude product to obtain the micro-channel array plate.

A bio emulsion fuel manufacturing apparatus and method using vegetable oil is provided, including an oil tank unit configured to refine a vegetable oil introduced from an oil inlet by using a coagulant agent and a centrifugal decanter; a water tank unit configured to pretreat a water introduced from a water inlet by using a water tank catalyst; a first HHO gas infuser unit configured to introduce nano-bubbles into the water inside the water tank; a mixed oil unit connected to the oil tank unit and the water tank unit, and configured to produce a mixed oil by using an inline mixer; an ionization catalyst unit connected to the mixed oil unit and configured to convert the mixed oil to a bio emulsion fuel by using an ionization catalyst group; and a second HHO gas infuser unit configured to introduce HHO gas into the bio emulsion fuel.

Provided herein is a method for preparing microcarrier particles, comprising the steps of allowing the dispersed phase liquid flow through a multi-hole plate at a low temperature to form liquid microspheres in a continuous phase, and enabling a synthetic polymer and/or natural biological macromolecules within the liquid microspheres to be subject to a curing reaction at a low temperature to form particles. Further provided herein are the method for preparing an emulsion and an apparatus and process system for preparing microcarrier particles, which can be used for preparing emulsions and microcarrier particles on a large scale.

A highly stable cannabinoid nanoparticle carrier composition for administration to a human made by incorporating non-ionic surfactants with cannabinoid oils and lipids, sonicating for a predetermined period of time at a predetermined amplification with an ultrasonic liquid processor until completely integrated; combining the mixture with a carrier fluid that includes ascorbic acid and distilled water; and further sonicating the mixture using an ultrasonic liquid processor at predetermined amplitude for a predetermined period of time at a controlled temperature, and thereby to create a CBD nanoemulsion. The composition is tailored using non-ionic surfactants to adsorb to the surface of the cannabinoid oil particles to advantageously affect electrokinetics and surface forces at the interface of the bioactive cannabinoid particles and the suspending liquid are controlled by tailoring the suspending liquid to maximize the zeta potential.

System for performing a flow-based assay. The system may comprise a droplet generator to produce an emulsion including droplets in a carrier fluid. The system also may comprise a thermocycler including two or more temperature-controlled zones and also including a channel connected to the droplet generator for receiving the emulsion. The channel may form a single-pass continuous fluid route traversing the temperature-controlled zones multiple times, such that droplets passing through the channel are thermally cycled. The system further may comprise a detection station downstream from the thermocycler and configured to detect a signal from the droplets after such droplets have been thermally cycled by passing through the channel.

The disclosure herein relates to a microfluidic emulsification device capable of being injection molded. The device may be used for digital droplet polymerase chain reaction (ddPCR). The emulsification device comprises: (a) a cylindrical outer part (4) with two open ends; (b) a cylindrical inner part (1) with a solid bottom and having a circumference sufficient to allow the inner part to be nested within the outer part of the emulsification device, wherein the inner part and the outer part are capable of sliding freely; (c) at least one groove on an interior surface of the outer part or on an exterior surface of the inner part, the groove having a height greater than a gap between the outer part and the inner part when nested; (d) at least one hole (3) in the inner part adjacent to the solid bottom; (e) a radial distribution channel (2) on the interior surface of the outer part or on the exterior surface of the inner part; and (f) a radial nozzle channel at the base of the interior surface of the outer part or at the base of the exterior surface of the inner part.

A method of producing a pharmaceutical gel emulsion, wherein the emulsion is an oil-in-water gel emulsion, comprising the steps of forming an oil-in-water emulsion comprising at least one pharmaceutically acceptable oil, at least one aqueous phase, at least one osmotic agent, at least one emulsifying agent, mixing a gelling polysaccharide with the oil-in-water emulsion and allowing the resulting mixture to form the pharmaceutical gel emulsion, optionally mixing an bioactive agent into the pharmaceutical gel emulsion.

Certain embodiments are directed to finite step emulsification device and/or methods that combine finite step emulsification with gradients of confinement for the formation of a 2D monolayer array of droplets with low size dispersion.