The methods described herein provide an improved approach for generating monodispersed droplets. Monodispersed droplets may be effectively obtained by using a plurality of particles to trigger the breakup of a jet, which can include, e.g., flowing in a channel of a microfluidic device a first fluid into a second fluid under stable jetting conditions to provide a jet of the first fluid in the second fluid, wherein the first fluid is immiscible with the second fluid; and introducing a plurality of particles into the jet of the first fluid triggering break-up of the jet of the first fluid and encapsulation of the plurality of particles in a plurality of monodispersed droplets of the first fluid in the second fluid.

A method for encapsulating active ingredients in liposomes having an active ingredient solution encapsulated with a bilayer composed of two monomolecular layers of amphiphilic compounds comprises: (a) providing the active ingredient solution; (b) providing an emulsion by emulsifying the active ingredient solution in a first liquid in the presence of the amphiphilic compound; (c) providing a liquid phase; (d) contacting the emulsion with the liquid phase to form a phase boundary; and (e) centrifuging the emulsion and the liquid phase that are in contact with one another via the phase boundary, wherein, on passage of the phase boundary, the amphiphilic compound enriched there is added onto the monomolecular inner layer to form a monomolecular outer layer, in order to create the bilayer.
The first liquid of the emulsion is chosen such that the solubility of the amphiphilic compound in the first liquid is not more than 1×10.sup.−4 mol/l.

An apparatus for stable nano emulsion of water in diesel fuel, including: a diesel fuel feeding unit (2), a water feeding unit (3), a magnetite nano-particles feeding unit (4); a mixing tank (7) in fluid communication with the diesel fuel feeding unit (2), with the water feeding unit (3) and with the magnetite nano-particles feeding unit (4); a recirculation conduit (8a, 8b, 8c, 8d) presenting opposite ends connected to the mixing tank (7). A pump (9) on the recirculation conduit (8a, 8b, 8c, 8d) and configured to recirculate a mixture of diesel fuel (F), water (W) and magnetite nano-particles (MNP). A dynamic magnetic field generator (25) is operationally coupled to the recirculation conduit (8a, 8b, 8c, 8d) and is configured to generate a dynamic magnetic field inside at least a section of the recirculation conduit (8a, 8b, 8c, 8d) to activate the magnetite nano-particles.

Certain embodiments are directed to finite step emulsification device and/or methods that combine finite step emulsification with gradients of confinement for the formation of a 2D monolayer array of droplets with low size dispersion.

Embodiments of the present application relate to batches of bupivacaine multivesicular liposomes (MVLs) prepared by a commercial manufacturing process using independently operating dual tangential flow filtration modules.

A lipid nanoparticles manufacturing chip includes a mixer unit for forming a mixed solution by mixing a first raw material containing an active ingredient and a second raw material containing a lipid, a dilution unit that is connected to the mixer unit and dilutes the mixed solution using a diluent solution to make a diluted mixed solution, and a concentration unit connected to the dilution unit and for obtaining a concentrated solution by concentrating lipid nanoparticles (LNP) from the diluted mixed solution.

In one embodiment, the present system describes a system wherein a first fluid is within a tank. An intermittent mixer is used for agitating the first fluid within the tank. At least one data acquisition device within the tank is capable of measuring at least one characteristic within the first fluid. In the embodiment, at least one data analyzer is capable of receiving the characteristics within the first fluid, comparing the characteristics within the first fluid to the characteristics of a second fluid, generating a data packet which contains a calculated operational speed and an operational time needed for the intermittent mixer to agitate the first fluid to obtain the characteristics of the second fluid and transmitting the data packet to the intermittent mixer. In this system the intermittent mixer is capable of altering the first fluid within the tank into the second fluid.

Embodiments described herein may be useful for optofluidic devices. For example, optofluidic devices using dynamic fluid lens materials represent an ideal platform to create versatile, reconfigurable, refractive optical components. For example, the articles described herein may be useful as fluidic tunable compound micro-lenses. Such compound micro-lenses may be composed of two or more components (e.g., two or more inner phases) that form stable bi-phase emulsion droplets in outer phases (e.g., aqueous media). In some embodiments, the articles described herein may be useful as light emitting droplets. Advantageously, the plurality of droplets may be configured such that light rays may modified (e.g., via stimulation of the droplets, exposure to an analyte such as a pathogen) to have a detectable emission intensity and/or angle of maximum emission intensity under a particular set of conditions.

Embodiments of the present application relate to batches of bupivacaine multivesicular liposomes (MVLs) prepared by a commercial manufacturing process using independently operating dual tangential flow filtration modules.

Embodiments of the present application relate to batches of bupivacaine multivesicular liposomes (MVLs) prepared by a commercial manufacturing process using independently operating dual tangential flow filtration modules.