Provided is a liquid supply method capable of accurately mixing a plurality of liquids without providing a complicated liquid supply control mechanism. A liquid supply method using an inspection chip 1, the liquid supply method including: a step of supplying a first liquid from an upstream flow path 4A to a combined flow path 7 and making the first liquid wet and spread on a wall surface of the combined flow path 7 to hold the first liquid in the combined flow path 7; a step of supplying a second liquid from the upstream flow path 4A to the combined flow path 7 and combining the first liquid and the second liquid; and a step of supplying the combined first liquid and the second liquid to a mixing flow path 8, and mixing the first liquid and the second liquid.

The present disclosure provides a system for the internal and external modification of nanoparticles in a continuous process. The system includes (a) a first inlet, (b) a second inlet, (c) a first pump in fluid communication with the first inlet, (d) a second pump in fluid communication with the second inlet, (e) a first flow meter positioned between the first pump and the first mixer, (f) a second flow meter positioned between the second pump and the first mixer, and (g) a mixing chamber in fluid communication with the first flow meter and the second flow meter, and (h) a first heat exchanger in fluid communication with the mixing chamber.

A method for controlling fluid ratio accuracy during a dual flow injection with a powered injection system is described. The method includes predicting a first capacitance volume of a first syringe comprising a first medical fluid and a second capacitance volume of a second syringe comprising a second medical fluid with a first capacitance correction factor and a second capacitance correction factor, respectively, selecting a ratio of the first medical fluid and the second medical fluid to be administered to a patient in the dual flow injection, determining a relative acceleration ratio of a first piston of the first syringe and a second piston of a second syringe based on the predicted first capacitance volume and the predicted second capacitance volume, wherein the relative acceleration ratio is selected to maintain the selected ratio of the first medical fluid and the second medical fluid during the dual flow injection, and injecting a mixture of a first medical fluid and a second medical fluid having the selected ratio with the powered injection system.

Microfluidic methods for barcoding nucleic acid target molecules to be analyzed, e.g., via nucleic acid sequencing techniques, are provided. Also provided are microfluidic, droplet-based methods of preparing nucleic acid barcodes for use in various barcoding applications. The methods described herein facilitate high-throughput sequencing of nucleic acid target molecules as well as single cell and single virus genomic, transcriptomic, and/or proteomic analysis/profiling. Systems and devices for practicing the subject methods are also provided.

Limit size lipid nanoparticles, methods for using the lipid nanoparticles, and methods and systems for making limit size lipid nanoparticles.

Disclosed material flow amplifiers have opposing amplifier bodies each with a profile that jointly defines an amplifier body (i.e., “clamshell configuration”). The amplifier body has a flow expander section and a vortex inducer section. A vortex chamber insert is within at least an interior space of the vortex inducer section. Such material flow amplifiers provide for flow of flowable material within a flow passage of a material flow conduit (e.g., a portion of a pipeline, tubing or the like) to have a cyclonic flow (i.e., vortex or swirling) profile. Advantageously, the cyclonic flow profile centralizes flow toward the central portion of the flow passage, thereby reducing laminar flow.

The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.

The invention describes a method for isolating one or more genetic elements encoding a gene product having a desired activity, comprising the steps of: (a) compartmentalising genetic elements into microcapsules; and (b) sorting the genetic elements which express the gene product having the desired activity; wherein at least one step is under microfluidic control. The invention enables the in vitro evolution of nucleic acids and proteins by repeated mutagenesis and iterative applications of the method of the invention.

The invention describes a method for isolating one or more genetic elements encoding a gene product having a desired activity, comprising the steps of: (a) compartmentalising genetic elements into microcapsules; and (b) sorting the genetic elements which express the gene product having the desired activity; wherein at least one step is under microfluidic control. The invention enables the in vitro evolution of nucleic acids and proteins by repeated mutagenesis and iterative applications of the method of the invention.

A bubble generating device is disclosed which includes: a bubble generator including a tubular body, a liquid introduction port, a gas introduction port, and a discharge port; and a gas supply unit including a gas supply port, a pressurized gas being supplied to the bubble generator through the gas supply port, wherein the flow passage of the bubble generator extends substantially along a same axis, a plurality of reduced diameter portions each having an inner diameter reduced are provided along a direction along which the liquid flows, and gas-liquid mixing portions are provided downstream of the respective reduced diameter portions in a contiguous manner, each of the gas-liquid mixing portions having an inner diameter larger than a minimum inner diameter of each of the plurality of reduced diameter portions, and the gas introduction port of the bubble generator is formed of a plurality of through holes.