The present disclosure relates to a fluid path member for generating nano-bubbles, and a fluid path integrator and a nano-bubble generator that use the same. The fluid path member may be configured such that a perimeter length of a cross-section of a fluid path is greater than a cross-sectional area of the fluid path so as to maximize a friction area per volume of fluid. In addition, the fluid path member may be configured such that a single fluid path is continuously formed over several tens of meters or more without a joint. Further, the fluid path member may be configured with a high density. Accordingly, the fluid path member may have improved ability to generate the nano-bubbles. A fluid path member configured to generate nano-bubbles according to some embodiments of the present disclosure includes a body formed as a bendable single tube, wherein the body is configured such that one or more dividing walls dividing a fluid path space inside a fluid path so as to expand a surface area and a friction area of a fluid are continuously integrally formed along a flow direction of the fluid, wherein the body is formed of a soft material of any one of silicone, rubber, and soft resin material so as to be freely bent and wound, and wherein the body is manufactured by extrusion molding such that the one or more dividing walls are continuously formed in a longitudinal direction of the body.

Material flow amplifiers comprise at least one helix vane within a vortex chamber of an amplifier body and at least a portion of an outer edge portion of the at least one helix vane is attached to an interior surface of the amplifier body. A centralizer tube is centrally located within the amplifier body and has at least a portion of an inner edge portion of the at least one helix vane is attached to an exterior surface thereof. Such material flow amplifiers provide for flow of flowable material within a flow passage of a material flow conduit to have a cyclonic flow (i.e., vortex or swirling) profile. Advantageously, the cyclonic flow profile centralizes flow toward the central portion of the flow passage, thereby reducing laminar flow to provide for increased flow rate in addition to reducing inner pipeline wear and energy consumption.

The present invention provides microfabricated substrates and methods of conducting reactions within these substrates. The reactions occur in plugs transported in the flow of a carrier-fluid.

The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.

The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.

The present invention generally relates to microfluidics, and, in particular, to systems and methods for coalescing or fusing droplets. In certain aspects, two or more droplets within a microfluidic channel are brought together and caused to coalesce without using electric fields or charges. For example, in certain embodiments, droplets stabilized with a surfactant may be disrupted, e.g., by exposing the droplets to a solvent able to alter the surfactant, which may partially destabilize the droplets and allow them to coalesce. In some instances, the droplets may also be physically disrupted to facilitate coalesce. In addition, in some cases, the positions of one or more droplets may be controlled within a channel using a groove in a wall of the channel. For example, a droplet may at least partially enter the groove such that the position of the droplet is at least partially controlled by the groove.

This in-line active and reverse calculating mass balance blending system can maintain a chemical at desired control points, such as with respect to concentration, temperature, and/or pressure, while the output flow rate is changing dynamically to a point of use. A blending unit is configured to receive and blend at least two species and deliver a mixture at selected concentrations to points of use. A controller can be configured to determine a mass balance to maintain the concentrations in the mixture using information from metrology systems and a flow in an output to the at least one point of use. The controller also can be configured to maintain a concentration in the mixture within a concentration range by controlling flow rates to the blending unit.

The present invention relates to an equipment and method for producing polymer pellets which comprise one or more polymer components and one or more further components, wherein in said process at least one of said one or more further components is incorporated into pellets by applying a liquid, which comprises said at least one component, onto said pellets.

A portable foam brush assembly having a base having a soap supply, a water supply, and an electrical supply. A foam brush wand is detachably dockable to the base and in electrical and fluid communication with the foam brush wand having battery pack, a peristaltic pump, a reservoir chamber, a foaming chamber, an air pump, a brush and a momentary release switch. The base water supply and the base soap supply, when activated, are configured to travel by a water supply hose and a soap supply hose to a tee in the base where they are combined in a combined hose to a base quick coupler that is connectable to a reciprocal foam brush wand quick coupler, which has a foam brush wand hose to deliver the water soap mixture to bladder in the foam brush wand.

Methods and systems for processing polynucleotides (e.g., DNA) are disclosed. A processing region includes one or more surfaces (e.g., particle surfaces) modified with ligands that retain polynucleotides under a first set of conditions (e.g., temperature and pH) and release the polynucleotides under a second set of conditions (e.g., higher temperature and/or more basic pH). The processing region can be used to, for example, concentrate polynucleotides of a sample and/or separate inhibitors of amplification reactions from the polynucleotides. Microfluidic devices with a processing region are disclosed.