The invention relates to treatment of a plant with one or more microorganisms for inhibiting a plant pathogen. More particularly, the invention relates to isolated Bacillus strains, and strains having all of the identifying characteristics of these strains, and combinations thereof, for a use comprising the above-mentioned use.

Endophytic bacteria, compositions comprising the same, and methods of use thereof are disclosed which suppress growth of aggressive, invasive weed species which grow in the same habitat of target plants of interest. Bioherbicides, compositions comprising the endophytic bacteria and methods of use thereof are disclosed which suppress growth of aggressive, invasive weed species which grow in the same habitat of target plants of interest.

Endophytic bacteria, compositions comprising the same, and methods of use thereof are disclosed which suppress growth of aggressive, invasive weed species which grow in the same habitat of target plants of interest. Bioherbicides, compositions comprising the endophytic bacteria and methods of use thereof are disclosed which suppress growth of aggressive, invasive weed species which grow in the same habitat of target plants of interest.

Endophytic bacteria, compositions comprising the same, and methods of use thereof are disclosed which suppress growth of aggressive, invasive weed species which grow in the same habitat of target plants of interest. Bioherbicides, compositions comprising the endophytic bacteria and methods of use thereof are disclosed which suppress growth of aggressive, invasive weed species which grow in the same habitat of target plants of interest.

A bacterial product may comprise strains Azospirillum oryzae AO.sub.512, Azospirillum lipoferum AL.sub.20, Pantoea dispersa PD.sub.2, Bacillus subtilis TU.sub.2, Lysinibacillus sphaericus 3S and Paenibacillus polymyxa SR.sub.17, in a total minimal concentration of 10.sup.8 CFU/g (Colony forming Units). These bacteria may be attached to an insoluble granular carrier formed of a mixture of natural clays and minerals. This formulation may preserve the viability of the microorganism for a long time while in storage, as well as after its application on the field. These microorganisms, as PGPR, may be capable of fixing atmospheric nitrogen, solubilize phosphates, potassium, as well as other minerals immobilized in the soil, which allows for a drastic reduction, and even for a complete elimination, of conventional fertilization methods. These bacteria are not harmful in any sense neither to human beings nor the environment.

A bacterial product may comprise strains Azospirillum oryzae AO.sub.512, Azospirillum lipoferum AL.sub.20, Pantoea dispersa PD.sub.2, Bacillus subtilis TU.sub.2, Lysinibacillus sphaericus 3S and Paenibacillus polymyxa SR.sub.17, in a total minimal concentration of 10.sup.8 CFU/g (Colony forming Units). These bacteria may be attached to an insoluble granular carrier formed of a mixture of natural clays and minerals. This formulation may preserve the viability of the microorganism for a long time while in storage, as well as after its application on the field. These microorganisms, as PGPR, may be capable of fixing atmospheric nitrogen, solubilize phosphates, potassium, as well as other minerals immobilized in the soil, which allows for a drastic reduction, and even for a complete elimination, of conventional fertilization methods. These bacteria are not harmful in any sense neither to human beings nor the environment.

A bacterial product may comprise strains Azospirillum oryzae AO.sub.512, Azospirillum lipoferum AL.sub.20, Pantoea dispersa PD.sub.2, Bacillus subtilis TU.sub.2, Lysinibacillus sphaericus 3S and Paenibacillus polymyxa SR.sub.17, in a total minimal concentration of 10.sup.8 CFU/g (Colony forming Units). These bacteria may be attached to an insoluble granular carrier formed of a mixture of natural clays and minerals. This formulation may preserve the viability of the microorganism for a long time while in storage, as well as after its application on the field. These microorganisms, as PGPR, may be capable of fixing atmospheric nitrogen, solubilize phosphates, potassium, as well as other minerals immobilized in the soil, which allows for a drastic reduction, and even for a complete elimination, of conventional fertilization methods. These bacteria are not harmful in any sense neither to human beings nor the environment.

The present invention concerns a process for preparing a composite of porous material/compound/hybrid organic-inorganic material having a 2:1 lamellar structure, said hybrid material having the following general formula I:
Na.sub.x[(Mg.sub.3)(Al.sub.x(RSi).sub.4−x)O.sub.8+x(OH).sub.2]  (I)
wherein
x is a number such that 0≤x<1.2 and
R represents a C.sub.1-C.sub.30 alkyl group, an aryl group, a (C.sub.1-C.sub.30 alkyl)aryl group or an O—(C.sub.1-C.sub.30 alkyl) group, it being possible for the alkyl group to be substituted with a group chosen from a phenyl, vinyl, aminopropyl or mercaptopropyl group,
and said compound being chosen from the group constituted of at least one active substance and at least one microorganism and mixtures thereof the process comprising:
a) the step of sol-gel synthesis of the hybrid organic-inorganic material having a 2:1 lamellar structure in the presence of the compound and of the porous material saturated with the compound;
b) the recovery of the composite. It also concerns a composite obtainable by means of this process, a composition comprising it and its use in particular for the fertilization of plants.

The present invention concerns a process for preparing a composite of porous material/compound/hybrid organic-inorganic material having a 2:1 lamellar structure, said hybrid material having the following general formula I:
Na.sub.x[(Mg.sub.3)(Al.sub.x(RSi).sub.4−x)O.sub.8+x(OH).sub.2]  (I)
wherein
x is a number such that 0≤x<1.2 and
R represents a C.sub.1-C.sub.30 alkyl group, an aryl group, a (C.sub.1-C.sub.30 alkyl)aryl group or an O—(C.sub.1-C.sub.30 alkyl) group, it being possible for the alkyl group to be substituted with a group chosen from a phenyl, vinyl, aminopropyl or mercaptopropyl group,
and said compound being chosen from the group constituted of at least one active substance and at least one microorganism and mixtures thereof the process comprising:
a) the step of sol-gel synthesis of the hybrid organic-inorganic material having a 2:1 lamellar structure in the presence of the compound and of the porous material saturated with the compound;
b) the recovery of the composite. It also concerns a composite obtainable by means of this process, a composition comprising it and its use in particular for the fertilization of plants.

The present invention concerns a process for preparing a composite of porous material/compound/hybrid organic-inorganic material having a 2:1 lamellar structure, said hybrid material having the following general formula I:
Na.sub.x[(Mg.sub.3)(Al.sub.x(RSi).sub.4−x)O.sub.8+x(OH).sub.2]  (I)
wherein
x is a number such that 0≤x<1.2 and
R represents a C.sub.1-C.sub.30 alkyl group, an aryl group, a (C.sub.1-C.sub.30 alkyl)aryl group or an O—(C.sub.1-C.sub.30 alkyl) group, it being possible for the alkyl group to be substituted with a group chosen from a phenyl, vinyl, aminopropyl or mercaptopropyl group,
and said compound being chosen from the group constituted of at least one active substance and at least one microorganism and mixtures thereof the process comprising:
a) the step of sol-gel synthesis of the hybrid organic-inorganic material having a 2:1 lamellar structure in the presence of the compound and of the porous material saturated with the compound;
b) the recovery of the composite. It also concerns a composite obtainable by means of this process, a composition comprising it and its use in particular for the fertilization of plants.