The disclosure provides a method of treating, preventing, and/or controlling plant diseases by applying the agricultural composition comprising a bacterial isolate belonging to Paenibacillus or Bacillus. Also, the disclosure provides a method of enhancing disease resistance of a plant seed by applying the agricultural composition to the plant seed, and an agricultural composition comprising the bacterial isolate.

The disclosure provides a method of treating, preventing, and/or controlling plant diseases by applying the agricultural composition comprising a bacterial isolate belonging to Paenibacillus or Bacillus. Also, the disclosure provides a method of enhancing disease resistance of a plant seed by applying the agricultural composition to the plant seed, and an agricultural composition comprising the bacterial isolate.

Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.

Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.

The present disclosure relates to a process for preparation of a peptide of sequence ID No. 1 having a formula C.sub.231H.sub.354N.sub.66O.sub.83 from an endophytic bacterium Paenibacillus peoriae IBSD35 isolated from the stem of Millettia pachycarpa. The genome size of Paenibacillus peoriae IBSD35 is 5.8 Mb, 45.6% GC content. The bioactive peptide designated as Peoriaerin IBSD35 of SEQ ID No. 1 was purified from the fermentation broth of P. peoriae strain IBSD35 and characterized with ESI (nano-spray)-TOF MS/MS spectrum. The peptide sequences were generated using MASCOT, MS-MS ion search. It has 54 amino acids residue and protein size is 5383.747 Da. The peptide of sequence ID No. 1 prepared by the present process exhibited broad spectrum of antibacterial and antifungal activity. It is highly resistant and stable to enzyme, detergent and environmental factors. It has the potential for drug development against AMR pathogens. It can also be used as food preservatives.

The present disclosure relates to a process for preparation of a peptide of sequence ID No. 1 having a formula C.sub.231H.sub.354N.sub.66O.sub.83 from an endophytic bacterium Paenibacillus peoriae IBSD35 isolated from the stem of Millettia pachycarpa. The genome size of Paenibacillus peoriae IBSD35 is 5.8 Mb, 45.6% GC content. The bioactive peptide designated as Peoriaerin IBSD35 of SEQ ID No. 1 was purified from the fermentation broth of P. peoriae strain IBSD35 and characterized with ESI (nano-spray)-TOF MS/MS spectrum. The peptide sequences were generated using MASCOT, MS-MS ion search. It has 54 amino acids residue and protein size is 5383.747 Da. The peptide of sequence ID No. 1 prepared by the present process exhibited broad spectrum of antibacterial and antifungal activity. It is highly resistant and stable to enzyme, detergent and environmental factors. It has the potential for drug development against AMR pathogens. It can also be used as food preservatives.

The present disclosure relates to the field of agrochemical compositions and formulations. In an aspect, the present disclosure provides for a composition in combination with a fibril or microfibril or nanofibril structuring agent. In yet another aspect, the disclosure provides for a composition comprising: (a) an agricultural bioactive material, a fungicide, insecticide, herbicide, and/or plant growth regulator; (b) a fibril or microfibril or nanofibril structuring agent; and (c) optionally, an auxiliary surface active agent. The disclosure further provides for a comprising a composition described herein mixed or combined with a liquid fertilizer.

The present disclosure relates to the field of agrochemical compositions and formulations. In an aspect, the present disclosure provides for a composition in combination with a fibril or microfibril or nanofibril structuring agent. In yet another aspect, the disclosure provides for a composition comprising: (a) an agricultural bioactive material, a fungicide, insecticide, herbicide, and/or plant growth regulator; (b) a fibril or microfibril or nanofibril structuring agent; and (c) optionally, an auxiliary surface active agent. The disclosure further provides for a comprising a composition described herein mixed or combined with a liquid fertilizer.

The present invention relates to a stabilized fungicidal composition comprising: a) a fusaricidin-producing Paenibacillus sp. strain; and b) a stabilizing agent selected from the group consisting of an amine, a quaternary ammonium compound, a salt of phosphate or citrate, a short chain polyol having from 2 to 10 carbon atoms, a urea, and combinations thereof. The present invention also provides agriculturally acceptable stable aqueous formulations comprising a fusaricidin-producing Paenibacillus sp. strain and a stabilizing agent and methods of controlling disease in plants with the fungicidal compositions and aqueous formulations.

The present invention relates to a stabilized fungicidal composition comprising: a) a fusaricidin-producing Paenibacillus sp. strain; and b) a stabilizing agent selected from the group consisting of an amine, a quaternary ammonium compound, a salt of phosphate or citrate, a short chain polyol having from 2 to 10 carbon atoms, a urea, and combinations thereof. The present invention also provides agriculturally acceptable stable aqueous formulations comprising a fusaricidin-producing Paenibacillus sp. strain and a stabilizing agent and methods of controlling disease in plants with the fungicidal compositions and aqueous formulations.